C75B3_ORYSJ
ID C75B3_ORYSJ Reviewed; 526 AA.
AC Q7G602; Q8S845;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Flavonoid 3'-monooxygenase CYP75B3 {ECO:0000305};
DE EC=1.14.14.82 {ECO:0000305|PubMed:18726614};
DE AltName: Full=Cytochrome P450 75B3 {ECO:0000303|PubMed:26082402};
DE AltName: Full=Flavonoid 3'-hydroxylase {ECO:0000303|PubMed:18726614};
DE Short=OsF3'H {ECO:0000303|PubMed:18726614};
GN Name=CYP75B3 {ECO:0000303|PubMed:26082402};
GN Synonyms=F3'H {ECO:0000303|PubMed:18726614};
GN OrderedLocusNames=Os10g0320100 {ECO:0000312|EMBL:BAF26252.1},
GN LOC_Os10g17260 {ECO:0000312|EMBL:AAP52914.1};
GN ORFNames=OsJ_31048 {ECO:0000312|EMBL:EAZ15637.1},
GN OSJNAa0053D03.2 {ECO:0000312|EMBL:AAN04937.1},
GN OSJNBa0053D03.12 {ECO:0000312|EMBL:AAM00948.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Liu N.N., Wang B.Q., Guan Y.;
RT "Cloning and analysis of the genes for flavonoid 3'-hydroxylase from Oryza
RT sativa Nipponbare.";
RL Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP FUNCTION, AND INDUCTION BY LIGHT.
RX PubMed=18726614; DOI=10.1007/s00425-008-0806-1;
RA Shih C.H., Chu H., Tang L.K., Sakamoto W., Maekawa M., Chu I.K., Wang M.,
RA Lo C.;
RT "Functional characterization of key structural genes in rice flavonoid
RT biosynthesis.";
RL Planta 228:1043-1054(2008).
RN [9]
RP FUNCTION.
RX PubMed=26082402; DOI=10.1104/pp.15.00566;
RA Lam P.Y., Liu H., Lo C.;
RT "Completion of tricin biosynthesis pathway in rice: cytochrome P450 75B4 is
RT a unique chrysoeriol 5'-hydroxylase.";
RL Plant Physiol. 168:1527-1536(2015).
CC -!- FUNCTION: Catalyzes the 3'-hydroxylation of the flavonoid B-ring to the
CC 3',4'-hydroxylated state (PubMed:18726614). Catalyzes the 3'-
CC hydroxylation of apigenin to generate luteolin (Probable).
CC {ECO:0000269|PubMed:18726614, ECO:0000305|PubMed:26082402}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3'-unsubstituted flavone + O2 + reduced [NADPH--hemoprotein
CC reductase] = a 3'-hydroxyflavone + H(+) + H2O + oxidized [NADPH--
CC hemoprotein reductase]; Xref=Rhea:RHEA:16337, Rhea:RHEA-COMP:11964,
CC Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:27741, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210, ChEBI:CHEBI:138726; EC=1.14.14.82;
CC Evidence={ECO:0000305|PubMed:18726614};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Induced by light. {ECO:0000269|PubMed:18726614}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; HQ876708; AEK31169.1; -; Genomic_RNA.
DR EMBL; AC021892; AAM00948.1; -; Genomic_DNA.
DR EMBL; AC131968; AAN04937.1; -; Genomic_DNA.
DR EMBL; DP000086; AAP52914.1; -; Genomic_DNA.
DR EMBL; AP008216; BAF26252.1; -; Genomic_DNA.
DR EMBL; AP014966; BAT10309.1; -; Genomic_DNA.
DR EMBL; CM000147; EAZ15637.1; -; Genomic_DNA.
DR EMBL; AK064736; BAG89180.1; -; mRNA.
DR RefSeq; XP_015613041.1; XM_015757555.1.
DR AlphaFoldDB; Q7G602; -.
DR SMR; Q7G602; -.
DR STRING; 4530.OS10T0320100-01; -.
DR PaxDb; Q7G602; -.
DR PRIDE; Q7G602; -.
DR EnsemblPlants; Os10t0320100-01; Os10t0320100-01; Os10g0320100.
DR GeneID; 4348304; -.
DR Gramene; Os10t0320100-01; Os10t0320100-01; Os10g0320100.
DR KEGG; osa:4348304; -.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_0_1; -.
DR InParanoid; Q7G602; -.
DR OMA; VPRCTAV; -.
DR OrthoDB; 702827at2759; -.
DR BioCyc; MetaCyc:MON-20527; -.
DR BRENDA; 1.14.14.82; 8948.
DR PlantReactome; R-OSA-1119322; Leucodelphinidin biosynthesis.
DR PlantReactome; R-OSA-1119415; Leucopelargonidin and leucocyanidin biosynthesis.
DR UniPathway; UPA00154; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000007752; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016711; F:flavonoid 3'-monooxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Flavonoid biosynthesis; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW NADP; Oxidoreductase; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..526
FT /note="Flavonoid 3'-monooxygenase CYP75B3"
FT /id="PRO_0000440770"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 461
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 526 AA; 57517 MW; A039C5A6DA3B3DC7 CRC64;
MDVVPLPLLL GSLAVSAAVW YLVYFLRGGS GGDAARKRRP LPPGPRGWPV LGNLPQLGDK
PHHTMCALAR QYGPLFRLRF GCAEVVVAAS APVAAQFLRG HDANFSNRPP NSGAEHVAYN
YQDLVFAPYG ARWRALRKLC ALHLFSAKAL DDLRAVREGE VALMVRNLAR QQAASVALGQ
EANVCATNTL ARATIGHRVF AVDGGEGARE FKEMVVELMQ LAGVFNVGDF VPALRWLDPQ
GVVAKMKRLH RRYDNMMNGF INERKAGAQP DGVAAGEHGN DLLSVLLARM QEEQKLDGDG
EKITETDIKA LLLNLFTAGT DTTSSTVEWA LAELIRHPDV LKEAQHELDT VVGRGRLVSE
SDLPRLPYLT AVIKETFRLH PSTPLSLPRE AAEECEVDGY RIPKGATLLV NVWAIARDPT
QWPDPLQYQP SRFLPGRMHA DVDVKGADFG LIPFGAGRRI CAGLSWGLRM VTLMTATLVH
GFDWTLANGA TPDKLNMEEA YGLTLQRAVP LMVQPVPRLL PSAYGV
