U5S1_CHICK
ID U5S1_CHICK Reviewed; 972 AA.
AC Q5F3X4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=116 kDa U5 small nuclear ribonucleoprotein component;
DE AltName: Full=Elongation factor Tu GTP-binding domain protein 2;
DE AltName: Full=U5 snRNP-specific protein, 116 kDa;
DE Short=U5-116 kDa;
GN Name=EFTUD2; Synonyms=SNRP116; ORFNames=RCJMB04_4m11;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
CC -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC spliceosome, including pre-catalytic, catalytic and post-catalytic
CC spliceosomal complexes (By similarity). Component of the U5 snRNP and
CC the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome (By
CC similarity). {ECO:0000250|UniProtKB:Q15029}.
CC -!- SUBUNIT: Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex,
CC a building block of the spliceosome (By similarity). Component of the
CC pre-catalytic, catalytic and post-catalytic spliceosome complexes (By
CC similarity). {ECO:0000250|UniProtKB:Q15029}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15029}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-G/EF-2
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU01059}.
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DR EMBL; AJ851526; CAH65160.1; -; mRNA.
DR RefSeq; NP_001026672.1; NM_001031501.1.
DR AlphaFoldDB; Q5F3X4; -.
DR SMR; Q5F3X4; -.
DR STRING; 9031.ENSGALP00000001456; -.
DR PaxDb; Q5F3X4; -.
DR Ensembl; ENSGALT00000001458; ENSGALP00000001456; ENSGALG00000000988.
DR GeneID; 428281; -.
DR KEGG; gga:428281; -.
DR CTD; 9343; -.
DR VEuPathDB; HostDB:geneid_428281; -.
DR eggNOG; KOG0468; Eukaryota.
DR GeneTree; ENSGT00940000155685; -.
DR HOGENOM; CLU_002794_11_2_1; -.
DR InParanoid; Q5F3X4; -.
DR OMA; GPDEMGP; -.
DR OrthoDB; 140796at2759; -.
DR PhylomeDB; Q5F3X4; -.
DR Reactome; R-GGA-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-GGA-72165; mRNA Splicing - Minor Pathway.
DR PRO; PR:Q5F3X4; -.
DR Proteomes; UP000000539; Chromosome 27.
DR Bgee; ENSGALG00000000988; Expressed in testis and 13 other tissues.
DR ExpressionAtlas; Q5F3X4; baseline and differential.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISS:UniProtKB.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0030623; F:U5 snRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR CDD; cd04098; eEF2_C_snRNP; 1.
DR CDD; cd04167; Snu114p; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR041095; EFG_II.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031950; EFTUD2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR044121; Snu114_GTP-bd.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR005517; Transl_elong_EFG/EF2_IV.
DR InterPro; IPR035655; U5-116kDa_C.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF14492; EFG_III; 1.
DR Pfam; PF03764; EFG_IV; 1.
DR Pfam; PF16004; EFTUD2; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SMART; SM00889; EFG_IV; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF54980; SSF54980; 2.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 2: Evidence at transcript level;
KW GTP-binding; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus;
KW Reference proteome; Spliceosome.
FT CHAIN 1..972
FT /note="116 kDa U5 small nuclear ribonucleoprotein
FT component"
FT /id="PRO_0000315998"
FT DOMAIN 127..409
FT /note="tr-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..49
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 136..143
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 204..208
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 258..261
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
SQ SEQUENCE 972 AA; 109478 MW; 8AC26F2335616FE0 CRC64;
MDTDLYDEFG NYIGPELDSD DEDDELGRES KELDELEDDD DDDDMGDHDE DHPGMEVVLH
EDKKYYPTAE EVYGPEVETI VQEEDTQPLT EPIIKPVKTK KFSLMEQTLP VTVYEMDFLA
DLMDNSELIR NVTLCGHLHH GKTCFVDCLI EQTHPEIRKR YDQDLCYTDI LFTEQERGVG
IKSTPVTIVL PDTKGKSFLF NIIDTPGHVN FSDEVTAGLR ISDGVVLFID AAEGVMLNTE
RLIKHAVQER LAVTVCINKI DRLILELKLP PTDAYYKLRH IVDEVNGLIS MYSTDENLVL
SPLLGNVCFS SSQYSICFTL GSFAKIYADT YGDINYQEFA KRLWGDIYFN PKTRKFTKKA
PTSSSQRSFV EFILEPLYKI LAQVVGDVDT TLPRTLDELG IHLTKEELKL NIRPLLRLVC
KKFFGEFTGF VDMCVQHIPS PKVGAKTKIE HTYTGGVDSD LGEAMSECDP DGPLMCHTTK
MYSTDDGVQF HAFGRVLSGT IHAGQPVKVL GENYTLEDEE DSQICTVGRL WISVARYHIE
VNRVPAGNWV LIEGVDQPIV KTATVTEPRG NEEAQIFRPL KFNTTSVIKI AVEPVNPSEL
PKMLDGLRKV NKSYPSLTTK VEESGEHVIL GTGELYLDCV MHDLRKMYSE IDIKVADPVV
TFCETVVETS SLKCFAETPN KKNKITMIAE PLEKGLAEDI ENEVVQITWN RKKLGEFFQT
KYDWDLLAAR SIWAFGPDAT GPNILVDDTL PSEVDKALLG SVKDSIVQGF QWGTREGPLC
DELIRNVKFK ILDAVIAQEP LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA
DCVSAVYTVL ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRTH TQGQAFSLSV
FHHWQIVPGD PLDKSIVIRP LEPQPAPHLA REFMIKTRRR KGLSEDVSIS KFFDDPMLLE
LAKQDVVLNY PM
