ID   C76AN_BETVU             Reviewed;         417 AA.
AC   P0DKI2;
DT   04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT   04-MAR-2015, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Inactive cytochrome P450 76AD1 {ECO:0000303|PubMed:22660548};
GN   Name=CYP76AD1 {ECO:0000303|PubMed:22660548};
OS   Beta vulgaris (Sugar beet).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Betoideae; Beta.
OX   NCBI_TaxID=161934;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=cv. US H20;
RX   PubMed=22660548; DOI=10.1038/ng.2297;
RA   Hatlestad G.J., Sunnadeniya R.M., Akhavan N.A., Gonzalez A., Goldman I.L.,
RA   McGrath J.M., Lloyd A.M.;
RT   "The beet R locus encodes a new cytochrome P450 required for red betalain
RT   production.";
RL   Nat. Genet. 44:816-820(2012).
CC   -!- FUNCTION: Inactive cytochrome unable to convert L-DOPA to cyclo-DOPA in
CC       the betalain pathway and producing a yellow mutant phenotype. A
CC       frameshift replaces 108 amino acids of the active protein found in red
CC       beets (AC I3PFJ5) with 27 new residues followed by a stop codon.
CC       {ECO:0000269|PubMed:22660548}.
CC   -!- PATHWAY: Pigment biosynthesis; betalain biosynthesis. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; HQ656021; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P0DKI2; -.
DR   SMR; P0DKI2; -.
DR   EnsemblPlants; KMT18892; KMT18892; BVRB_2g029930.
DR   Gramene; KMT18892; KMT18892; BVRB_2g029930.
DR   PhylomeDB; P0DKI2; -.
DR   UniPathway; UPA00278; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
PE   3: Inferred from homology;
KW   Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..417
FT                   /note="Inactive cytochrome P450 76AD1"
FT                   /id="PRO_0000431984"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   417 AA;  47288 MW;  0DE0060E335CEF9A CRC64;
     MDHATLAMIL AIWFISFHFI KLLFSQQTTK LLPPGPKPLP IIGNILEVGK KPHRSFANLA
     KIHGPLISLR LGSVTTIVVS SADVAKEMFL KKDHPLSNRT IPNSVTAGDH HKLTMSWLPV
     SPKWRNFRKI TAVHLLSPQR LDACQTFRHA KVQQLYEYVQ ECAQKGQAVD IGKAAFTTSL
     NLLSKLFFSV ELAHHKSHTS QEFKELIWNI MEDIGKPNYA DYFPILGCVD PSGIRRRLAC
     SFDKLIAVFQ SIICERLAPD SSTATTTTTD DVLDVLLQLF KQNELTMGEI NHLLVDIFDA
     GTDTTSSTFE WVMAELIRNP EMMEKAQEEI KQVLGKDKQI QESDIINLPY LQAIIKETLR
     LHPPTVFLLP RKADTDVELY GYIVPKDAQI KYLLTYGLLE EILMHGKMLI FFRPKDL