ID   U6BW_CONRA              Reviewed;          33 AA.
AC   P58804;
DT   02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2002, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Bromosleeper peptide;
OS   Conus radiatus (Rayed cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Phasmoconus.
OX   NCBI_TaxID=61198;
RN   [1]
RP   PROTEIN SEQUENCE, BROMINATION AT TRP-1, HYDROXYLATION AT PRO-21 AND PRO-22,
RP   GAMMA-CARBOXYGLUTAMATION AT GLU-6; GLU-8; GLU-9 AND GLU-29, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=9030520; DOI=10.1074/jbc.272.8.4689;
RA   Craig A.G., Jimenez E.C., Dykert J., Nielsen D.B., Gulyas J.,
RA   Abogadie F.C., Porter J., Rivier J.E., Cruz L.J., Olivera B.M.,
RA   McIntosh J.M.;
RT   "A novel post-translational modification involving bromination of
RT   tryptophan. Identification of the residue, L-6-bromotryptophan, in peptides
RT   from Conus imperialis and Conus radiatus venom.";
RL   J. Biol. Chem. 272:4689-4698(1997).
CC   -!- FUNCTION: Induces sleep-like symptoms in mice of all ages.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C).
CC   -!- MASS SPECTROMETRY: Mass=3916.4; Method=LSI;
CC       Evidence={ECO:0000269|PubMed:9030520};
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DR   PIR; B58512; B58512.
DR   AlphaFoldDB; P58804; -.
DR   ConoServer; 1811; Bromosleeper peptide.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Bromination; Direct protein sequencing; Disulfide bond;
KW   Gamma-carboxyglutamic acid; Hydroxylation; Knottin; Neurotoxin; Secreted;
KW   Toxin.
FT   PEPTIDE         1..33
FT                   /note="Bromosleeper peptide"
FT                   /id="PRO_0000044880"
FT   MOD_RES         1
FT                   /note="6'-bromotryptophan"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   MOD_RES         6
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   MOD_RES         8
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   MOD_RES         9
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   MOD_RES         21
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   MOD_RES         22
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   MOD_RES         29
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   DISULFID        7..19
FT                   /evidence="ECO:0000250"
FT   DISULFID        11..27
FT                   /evidence="ECO:0000250"
FT   DISULFID        18..31
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   33 AA;  3636 MW;  8AF54168A0554EDF CRC64;
     WATIDECEET CNVTFKTCCG PPGDWQCVEA CPV