ID   U6B_CONPI               Reviewed;          32 AA.
AC   P0CH18;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=Conotoxin pr6b;
OS   Conus parius (Cone snail).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Phasmoconus.
OX   NCBI_TaxID=505247;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, HYDROXYLATION AT PRO-6; PRO-13; PRO-20 AND
RP   PRO-29, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=20570703; DOI=10.1016/j.peptides.2010.05.020;
RA   Jimenez E.C., Olivera B.M.;
RT   "Divergent M- and O-superfamily peptides from venom of fish-hunting Conus
RT   parius.";
RL   Peptides 31:1678-1683(2010).
CC   -!- FUNCTION: Intraperitoneal injection into fish (0.5 nmol) provokes
CC       vertical suspension and paralysis after 6 min.
CC       {ECO:0000269|PubMed:20570703}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C).
CC   -!- MASS SPECTROMETRY: Mass=3623.8; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:20570703};
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DR   AlphaFoldDB; P0CH18; -.
DR   SMR; P0CH18; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Hydroxylation; Knottin;
KW   Neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..32
FT                   /note="Conotoxin pr6b"
FT                   /id="PRO_0000397116"
FT   MOD_RES         6
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:20570703"
FT   MOD_RES         13
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:20570703"
FT   MOD_RES         20
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:20570703"
FT   MOD_RES         29
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:20570703"
FT   DISULFID        7..18
FT                   /evidence="ECO:0000250"
FT   DISULFID        14..23
FT                   /evidence="ECO:0000250"
FT   DISULFID        17..31
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   32 AA;  3564 MW;  EFAC7B524506B5D1 CRC64;
     FGSFIPCAHK GEPCTICCRP LRCHEEKTPT CV