U6VN_CONVE
ID U6VN_CONVE Reviewed; 33 AA.
AC P83301;
DT 13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-2002, sequence version 1.
DT 25-MAY-2022, entry version 58.
DE RecName: Full=Omega-conotoxin-like Vn2;
DE AltName: Full=Conotoxin Vn2 {ECO:0000303|PubMed:22728460};
DE AltName: Full=Conotoxin-Vn {ECO:0000303|PubMed:18266261};
DE AltName: Full=Vn6A;
OS Conus ventricosus (Mediterranean cone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Lautoconus.
OX NCBI_TaxID=117992;
RN [1]
RP PROTEIN SEQUENCE, AMIDATION AT PRO-33, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=18266261; DOI=10.1002/jssc.200700448;
RA Romeo C., Di Francesco L., Oliverio M., Palazzo P., Massilia G.R.,
RA Ascenzi P., Polticelli F., Schinina M.E.;
RT "Conus ventricosus venom peptides profiling by HPLC-MS: a new insight in
RT the intraspecific variation.";
RL J. Sep. Sci. 31:488-498(2008).
RN [2]
RP FUNCTION, TOXIC DOSE, AND MUTAGENESIS OF ASP-2.
RX PubMed=22728460; DOI=10.1016/j.toxicon.2012.06.008;
RA Spiezia M.C., Chiarabelli C., Polticelli F.;
RT "Recombinant expression and insecticidal properties of a Conus ventricosus
RT conotoxin-GST fusion protein.";
RL Toxicon 60:744-751(2012).
CC -!- FUNCTION: Omega-conotoxins act at presynaptic membranes, they bind and
CC block voltage-gated calcium channels (Cav) (By similarity). Has strong
CC insecticidal properties at a dose of only 100 pmol/g of body weight
CC (when injected into the haemocoel of the wax moth G. mellonella
CC larvae). Provoques tremor and uncontrolled movements in insect larvae,
CC that are typical symptoms caused by neurotoxins (PubMed:22728460). On
CC fish G.niger, intraperitoneal injection of the toxin causes full
CC extension of the fins, change in posture, breathing difficulties (at 30
CC and 100 pmol/g body weight) and death (at 100 pmol/g body weight)
CC (PubMed:22728460). {ECO:0000250, ECO:0000269|PubMed:22728460}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18266261}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000250}.
CC -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C).
CC -!- TOXIC DOSE: LD(50) is more than 100 pmol/g body weight (at 24 hours)
CC when injected into the haemocoel of the wax moth G. mellonella larvae.
CC {ECO:0000269|PubMed:22728460}.
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DR AlphaFoldDB; P83301; -.
DR SMR; P83301; -.
DR TCDB; 8.B.4.1.1; the conotoxin t (conotoxin t) family.
DR ConoServer; 1390; Vn6A.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Calcium channel impairing toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin;
KW Presynaptic neurotoxin; Secreted; Toxin;
KW Voltage-gated calcium channel impairing toxin.
FT PEPTIDE 1..33
FT /note="Omega-conotoxin-like Vn2"
FT /id="PRO_0000044881"
FT MOD_RES 33
FT /note="Proline amide"
FT /evidence="ECO:0000269|PubMed:18266261"
FT DISULFID 3..20
FT /evidence="ECO:0000250"
FT DISULFID 10..24
FT /evidence="ECO:0000250"
FT DISULFID 19..28
FT /evidence="ECO:0000250"
FT MUTAGEN 2
FT /note="D->A: Increase in toxicity in insect larvae."
FT /evidence="ECO:0000269|PubMed:22728460"
SQ SEQUENCE 33 AA; 3546 MW; 662171EF59F5DA5A CRC64;
EDCIAVGQLC VFWNIGRPCC SGLCVFACTV KLP
