U70G2_CROXC
ID U70G2_CROXC Reviewed; 470 AA.
AC A0A2Z5CV93;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2018, sequence version 1.
DT 03-AUG-2022, entry version 13.
DE RecName: Full=Myricetin 3-O-rhamnoside 1,2-glucosyltransferase UGT709G2;
DE EC=2.4.1.- {ECO:0000269|PubMed:29967287};
DE AltName: Full=UDP-glucosyltransferase 2 {ECO:0000303|PubMed:31004005};
DE Short=CcUGT2 {ECO:0000303|PubMed:31004005};
GN Name=UGT709G2 {ECO:0000303|PubMed:29967287};
GN Synonyms=UGT2 {ECO:0000303|PubMed:31004005};
OS Crocosmia x crocosmiiflora (Montbretia) (Crocosmia aurea x Crocosmia
OS pottsii).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Iridaceae;
OC Crocoideae; Freesieae; Crocosmia.
OX NCBI_TaxID=1053288;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND TISSUE
RP SPECIFICITY.
RX PubMed=29967287; DOI=10.1105/tpc.18.00406;
RA Irmisch S., Jo S., Roach C.R., Jancsik S., Man Saint Yuen M., Madilao L.L.,
RA O'Neil-Johnson M., Williams R., Withers S.G., Bohlmann J.;
RT "Discovery of UDP-glycosyltransferases and BAHD-acyltransferases involved
RT in the biosynthesis of the antidiabetic plant metabolite montbretin A.";
RL Plant Cell 30:1864-1886(2018).
RN [2]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=31004005; DOI=10.1104/pp.19.00254;
RA Irmisch S., Ruebsam H., Jancsik S., Man Saint Yuen M., Madilao L.L.,
RA Bohlmann J.;
RT "Flavonol biosynthesis genes and their use in engineering the plant
RT antidiabetic metabolite montbretin A.";
RL Plant Physiol. 180:1277-1290(2019).
CC -!- FUNCTION: Glucosyltransferase involved in montbretin A (MbA)
CC biosynthesis (PubMed:29967287, PubMed:31004005). Catalyzes the
CC glucosylation of myricetin 3-O-alpha-L-rhamnoside (MR) to produce
CC myricetin 3-O-[beta-D-glucosyl-(1->2)-alpha-L-rhamnoside] (MRG), a
CC precursor of MbA (PubMed:29967287, PubMed:31004005). MbA is a potent
CC inhibitor of human pancreatic alpha-amylase and is being developed as
CC drug candidate to treat type-2 diabetes (PubMed:29967287,
CC PubMed:31004005). In vitro, is able to transfer UDP-xylose with 50-fold
CC less efficiency compared with UDP-glucose (PubMed:29967287). In vitro,
CC can use myricetin 3-O-glucoside and quercetin 3-O-glucoside as
CC substrates, although these two flavonoids may not be physiological
CC substrates in vivo (PubMed:29967287). {ECO:0000269|PubMed:29967287,
CC ECO:0000269|PubMed:31004005}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=myricetin 3-O-alpha-L-rhamnoside + UDP-alpha-D-glucose = H(+)
CC + myricetin 3-O-[beta-D-glucosyl-(1->2)-alpha-L-rhamnoside] + UDP;
CC Xref=Rhea:RHEA:61148, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:144429, ChEBI:CHEBI:144432;
CC Evidence={ECO:0000269|PubMed:29967287, ECO:0000269|PubMed:31004005};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61149;
CC Evidence={ECO:0000269|PubMed:29967287, ECO:0000269|PubMed:31004005};
CC -!- PATHWAY: Flavonoid metabolism. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in young cromes.
CC {ECO:0000269|PubMed:29967287}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; MG938543; AXB26716.1; -; mRNA.
DR AlphaFoldDB; A0A2Z5CV93; -.
DR SMR; A0A2Z5CV93; -.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Transferase.
FT CHAIN 1..470
FT /note="Myricetin 3-O-rhamnoside 1,2-glucosyltransferase
FT UGT709G2"
FT /id="PRO_0000448218"
FT BINDING 20
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q7XT97"
FT BINDING 287
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q7XT97"
FT BINDING 357..365
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q7XT97"
FT BINDING 381..382
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q7XT97"
SQ SEQUENCE 470 AA; 52699 MW; B3C89BA4DA474CBC CRC64;
MAEKEAKHHV VLFPLPGQGH IGSMLKLAQL LSTAGFYITF VHTERNYRRF LLTSTSFNVP
KFRFRTIPDG FPDNDPRSPL PFIELQESLD TKCKGYYREV LVAVDEEWPP VTCVVADTAL
PLALEVPEEL GIPVMILAPH SAGSILTGYS IPQLIQGGEF PFPEDADMDE LLQGVLGLEG
IVRRRDMSVR GFKSIDSPFV RFEVKMNQNL SRGRALILNT TESMDSLALR HIRSICPTTY
TLGPFHVLLR NIKDQSHSAS LSEEDRSCIA WLDTKPNKSV VYVSFGSLAA MSREAFLEFQ
QGLLDSGYHF LWVIRPDMVE GGLEECELTA SERRYFVKWA PQEEVLAHPA VGCFLTHSGW
NSTLESIYAG VPMICWPFFA DQLINSRFVS EVWKIALDMK DLCGRSYVER MVKEVMSGEK
GKELRKSICE MADMVKKSAE EGGSSYTNFK ELIGHIKSLS LPACSSTSGF
