ID   U71A2_PANGI             Reviewed;         473 AA.
AC   A0A0A7HB61;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   04-MAR-2015, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=UDP-glycosyltransferase 71A27 {ECO:0000303|Ref.1};
DE            Short=PgUGT71A27 {ECO:0000303|Ref.1};
DE            EC=2.4.1.363 {ECO:0000269|PubMed:25320211};
GN   Name=UGT71A27 {ECO:0000303|Ref.1};
OS   Panax ginseng (Korean ginseng).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Araliaceae; Panax.
OX   NCBI_TaxID=4054;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Jung S.-C., Kim W., Lim S., Choi G., Kim S.C.;
RT   "Panax ginseng UGTs increased expression level in leaf and root by MeJA.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   INDUCTION BY METHYL JASMONATE.
RX   PubMed=25320211; DOI=10.1093/pcp/pcu147;
RA   Jung S.-C., Kim W., Park S.C., Jeong J., Park M.K., Lim S., Lee Y.,
RA   Im W.-T., Lee J.H., Choi G., Kim S.C.;
RT   "Two ginseng UDP-glycosyltransferases synthesize ginsenoside Rg3 and Rd.";
RL   Plant Cell Physiol. 55:2177-2188(2014).
RN   [3]
RP   REVIEW.
RX   PubMed=29509695; DOI=10.3390/molecules23030589;
RA   Yang J.-L., Hu Z.-F., Zhang T.-T., Gu A.-D., Gong T., Zhu P.;
RT   "Progress on the studies of the key enzymes of ginsenoside biosynthesis.";
RL   Molecules 23:0-0(2018).
CC   -!- FUNCTION: Component of the triterpene saponins (e.g. PPD-type
CC       ginsenosides or panaxosides) biosynthetic pathways (PubMed:25320211).
CC       Glycosyltransferase that catalyzes the biosynthesis of compound K from
CC       protopanaxadiol (PPD) (PubMed:25320211). {ECO:0000303|PubMed:25320211}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(20S)-protopanaxadiol + UDP-alpha-D-glucose = (20S)-
CC         ginsenoside C-K + H(+) + UDP; Xref=Rhea:RHEA:57976,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC         ChEBI:CHEBI:75950, ChEBI:CHEBI:77146; EC=2.4.1.363;
CC         Evidence={ECO:0000269|PubMed:25320211};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57977;
CC         Evidence={ECO:0000269|PubMed:25320211};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000305}.
CC   -!- INDUCTION: Induced by methyl jasmonate (MeJA).
CC       {ECO:0000269|PubMed:25320211}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; KM491309; AIZ00429.1; -; mRNA.
DR   AlphaFoldDB; A0A0A7HB61; -.
DR   SMR; A0A0A7HB61; -.
DR   BioCyc; MetaCyc:MON-20532; -.
DR   UniPathway; UPA00213; -.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   Pfam; PF00201; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Isoprene biosynthesis; Transferase.
FT   CHAIN           1..473
FT                   /note="UDP-glycosyltransferase 71A27"
FT                   /id="PRO_0000446964"
FT   BINDING         278
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   BINDING         344..345
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   BINDING         362..370
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   BINDING         384..387
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
SQ   SEQUENCE   473 AA;  53230 MW;  09D6722ACB7C957A CRC64;
     MKSELIFLPA PAIGHLVGMV EMAKLFISRH ENLSVTVLIA KFYMDTGVDN YNKSLLTNPT
     PRLTIVNLPE TDPQNYMLKP RHAIFPSVIE TQKTHVRDII SGMTQSESTR VVGLLADLLF
     INIMDIANEF NVPTYVYSPA GAGHLGLAFH LQTLNDKKQD VTEFRNSDTE LLVPSFANPV
     PAEVLPSMYV DKEGGYDYLF SLFRRCRESK AIIINTFEEL EPYAINSLRM DSMIPPIYPV
     GPILNLNGDG QNSDEAAVIL GWLDDQPPSS VVFLCFGSYG SFQENQVKEI AMGLERSGHR
     FLWSLRPSIP KGETKLQLKY SNLKEILPVG FLDRTSCVGK VIGWAPQVAV LAHKAVGGFV
     SHCGWNSILE SVWYDMSVAT WPMYGEQQLN AFEMVKELGL AVEIEVDYRN EYNKTGFIVR
     ADEIETKIKK LMMDEKNSEI RKKVKEMKEK SRVAMSENGS SYTSLAKLFE KIM