U71B1_ARATH
ID U71B1_ARATH Reviewed; 473 AA.
AC Q9LSY9; Q9ASY6;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=UDP-glycosyltransferase 71B1;
DE EC=2.4.1.-;
DE AltName: Full=Flavonol 3-O-glucosyltransferase UGT71B1;
DE EC=2.4.1.91;
GN Name=UGT71B1; OrderedLocusNames=At3g21750; ORFNames=MSD21.6, MSD21.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
RN [6]
RP FUNCTION.
RX PubMed=11641410; DOI=10.1074/jbc.m109287200;
RA Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J.,
RA Bowles D.J.;
RT "The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-
RT hydroxybenzoic acid, and other benzoates.";
RL J. Biol. Chem. 277:586-592(2002).
RN [7]
RP FUNCTION.
RX PubMed=15352060; DOI=10.1002/bit.20154;
RA Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.;
RT "Arabidopsis glycosyltransferases as biocatalysts in fermentation for
RT regioselective synthesis of diverse quercetin glucosides.";
RL Biotechnol. Bioeng. 87:623-631(2004).
CC -!- FUNCTION: Possesses quercetin 3-O-glucosyltransferase activity in
CC vitro. Also active in vitro on benzoates and benzoate derivatives.
CC {ECO:0000269|PubMed:11641410, ECO:0000269|PubMed:15352060}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a flavonol + UDP-alpha-D-glucose = a flavonol 3-O-beta-D-
CC glucoside + H(+) + UDP; Xref=Rhea:RHEA:22300, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16816, ChEBI:CHEBI:28802, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885; EC=2.4.1.91;
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK32764.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB025634; BAB02837.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76548.1; -; Genomic_DNA.
DR EMBL; AF361596; AAK32764.1; ALT_FRAME; mRNA.
DR EMBL; AK227147; BAE99192.1; -; mRNA.
DR RefSeq; NP_188812.1; NM_113070.3.
DR AlphaFoldDB; Q9LSY9; -.
DR SMR; Q9LSY9; -.
DR STRING; 3702.AT3G21750.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; Q9LSY9; -.
DR PRIDE; Q9LSY9; -.
DR ProteomicsDB; 228638; -.
DR DNASU; 821729; -.
DR EnsemblPlants; AT3G21750.1; AT3G21750.1; AT3G21750.
DR GeneID; 821729; -.
DR Gramene; AT3G21750.1; AT3G21750.1; AT3G21750.
DR KEGG; ath:AT3G21750; -.
DR Araport; AT3G21750; -.
DR TAIR; locus:2093079; AT3G21750.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_3_2_1; -.
DR InParanoid; Q9LSY9; -.
DR OMA; RKSEVHQ; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; Q9LSY9; -.
DR BioCyc; ARA:AT3G21750-MON; -.
DR BioCyc; MetaCyc:AT3G21750-MON; -.
DR PRO; PR:Q9LSY9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LSY9; baseline and differential.
DR Genevisible; Q9LSY9; AT.
DR GO; GO:0102360; F:daphnetin 3-O-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047893; F:flavonol 3-O-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102425; F:myricetin 3-O-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IDA:TAIR.
DR GO; GO:0035251; F:UDP-glucosyltransferase activity; IDA:TAIR.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..473
FT /note="UDP-glycosyltransferase 71B1"
FT /id="PRO_0000409047"
FT BINDING 273
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 342..344
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 359..367
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 381..384
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 473 AA; 52666 MW; 42B7925984C350B0 CRC64;
MKVELVFIPS PGVGHIRATT ALAKLLVASD NRLSVTLIVI PSRVSDDASS SVYTNSEDRL
RYILLPARDQ TTDLVSYIDS QKPQVRAVVS KVAGDVSTRS DSRLAGIVVD MFCTSMIDIA
DEFNLSAYIF YTSNASYLGL QFHVQSLYDE KELDVSEFKD TEMKFDVPTL TQPFPAKCLP
SVMLNKKWFP YVLGRARSFR ATKGILVNSV ADMEPQALSF FSGGNGNTNI PPVYAVGPIM
DLESSGDEEK RKEILHWLKE QPTKSVVFLC FGSMGGFSEE QAREIAVALE RSGHRFLWSL
RRASPVGNKS NPPPGEFTNL EEILPKGFLD RTVEIGKIIS WAPQVDVLNS PAIGAFVTHC
GWNSILESLW FGVPMAAWPI YAEQQFNAFH MVDELGLAAE VKKEYRRDFL VEEPEIVTAD
EIERGIKCAM EQDSKMRKRV MEMKDKLHVA LVDGGSSNCA LKKFVQDVVD NVP
