U71B6_ARATH
ID U71B6_ARATH Reviewed; 479 AA.
AC Q9LSY6; A1L4W4;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=UDP-glycosyltransferase 71B6;
DE EC=2.4.1.-;
DE AltName: Full=Abscisic acid glycosyltransferase;
GN Name=UGT71B6; OrderedLocusNames=At3g21780; ORFNames=MSD21.11, MSD21.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 49-479.
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
RN [5]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=16083885; DOI=10.1016/j.febslet.2005.06.084;
RA Priest D.M., Jackson R.G., Ashford D.A., Abrams S.R., Bowles D.J.;
RT "The use of abscisic acid analogues to analyse the substrate selectivity of
RT UGT71B6, a UDP-glycosyltransferase of Arabidopsis thaliana.";
RL FEBS Lett. 579:4454-4458(2005).
CC -!- FUNCTION: Glucosyltransferase that glucosylates the (+) enantiomer of
CC abscisic acid ((+)-ABA). Is not active on structural analogs with
CC alterations to the 8'- and 9'- methyl groups.
CC {ECO:0000269|PubMed:16083885}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=280 uM for ABA {ECO:0000269|PubMed:16083885};
CC Vmax=1.8 nmol/sec/mg enzyme {ECO:0000269|PubMed:16083885};
CC pH dependence:
CC Optimum pH is 6.5-7.0. {ECO:0000269|PubMed:16083885};
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB025634; BAB02840.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76551.1; -; Genomic_DNA.
DR EMBL; BT029751; ABM06021.1; -; mRNA.
DR RefSeq; NP_188815.2; NM_113073.3.
DR AlphaFoldDB; Q9LSY6; -.
DR SMR; Q9LSY6; -.
DR STRING; 3702.AT3G21780.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR iPTMnet; Q9LSY6; -.
DR PaxDb; Q9LSY6; -.
DR PRIDE; Q9LSY6; -.
DR ProteomicsDB; 242806; -.
DR DNASU; 821732; -.
DR EnsemblPlants; AT3G21780.1; AT3G21780.1; AT3G21780.
DR GeneID; 821732; -.
DR Gramene; AT3G21780.1; AT3G21780.1; AT3G21780.
DR KEGG; ath:AT3G21780; -.
DR Araport; AT3G21780; -.
DR TAIR; locus:2093104; AT3G21780.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_3_2_1; -.
DR OMA; MAIWPLY; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; Q9LSY6; -.
DR BioCyc; ARA:AT3G21780-MON; -.
DR BioCyc; MetaCyc:AT3G21780-MON; -.
DR PRO; PR:Q9LSY6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LSY6; differential.
DR Genevisible; Q9LSY6; AT.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IDA:TAIR.
DR GO; GO:0046345; P:abscisic acid catabolic process; TAS:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IEP:TAIR.
DR GO; GO:0006970; P:response to osmotic stress; IEP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IEP:TAIR.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..479
FT /note="UDP-glycosyltransferase 71B6"
FT /id="PRO_0000409050"
FT BINDING 275
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 342..344
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 359..367
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 381..384
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 479 AA; 53844 MW; 003BE74556D97DF1 CRC64;
MKIELVFIPS PAISHLMATV EMAEQLVDKN DNLSITVIII SFSSKNTSMI TSLTSNNRLR
YEIISGGDQQ PTELKATDSH IQSLKPLVRD AVAKLVDSTL PDAPRLAGFV VDMYCTSMID
VANEFGVPSY LFYTSNAGFL GLLLHIQFMY DAEDIYDMSE LEDSDVELVV PSLTSPYPLK
CLPYIFKSKE WLTFFVTQAR RFRETKGILV NTVPDLEPQA LTFLSNGNIP RAYPVGPLLH
LKNVNCDYVD KKQSEILRWL DEQPPRSVVF LCFGSMGGFS EEQVRETALA LDRSGHRFLW
SLRRASPNIL REPPGEFTNL EEILPEGFFD RTANRGKVIG WAEQVAILAK PAIGGFVSHG
GWNSTLESLW FGVPMAIWPL YAEQKFNAFE MVEELGLAVE IKKHWRGDLL LGRSEIVTAE
EIEKGIICLM EQDSDVRKRV NEISEKCHVA LMDGGSSETA LKRFIQDVTE NIAWSETES
