ID   U71B8_ARATH             Reviewed;         480 AA.
AC   Q9LSY4;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=UDP-glycosyltransferase 71B8;
DE            EC=2.4.1.-;
GN   Name=UGT71B8; OrderedLocusNames=At3g21800; ORFNames=MSD21.11, MSD21.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA   Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT   "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT   Arabidopsis thaliana.";
RL   J. Biol. Chem. 276:4338-4343(2001).
RN   [4]
RP   FUNCTION.
RX   PubMed=15352060; DOI=10.1002/bit.20154;
RA   Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.;
RT   "Arabidopsis glycosyltransferases as biocatalysts in fermentation for
RT   regioselective synthesis of diverse quercetin glucosides.";
RL   Biotechnol. Bioeng. 87:623-631(2004).
CC   -!- FUNCTION: Possesses low quercetin 3-O-glucosyltransferase and 4'-O-
CC       glucosyltransferase activities in vitro. {ECO:0000269|PubMed:15352060}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AB025634; BAB02842.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE76553.1; -; Genomic_DNA.
DR   RefSeq; NP_188817.1; NM_113075.3.
DR   AlphaFoldDB; Q9LSY4; -.
DR   SMR; Q9LSY4; -.
DR   STRING; 3702.AT3G21800.1; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   PaxDb; Q9LSY4; -.
DR   PRIDE; Q9LSY4; -.
DR   ProteomicsDB; 242606; -.
DR   EnsemblPlants; AT3G21800.1; AT3G21800.1; AT3G21800.
DR   GeneID; 821734; -.
DR   Gramene; AT3G21800.1; AT3G21800.1; AT3G21800.
DR   KEGG; ath:AT3G21800; -.
DR   Araport; AT3G21800; -.
DR   TAIR; locus:2093034; AT3G21800.
DR   eggNOG; KOG1192; Eukaryota.
DR   HOGENOM; CLU_001724_3_2_1; -.
DR   InParanoid; Q9LSY4; -.
DR   OMA; ISFLIME; -.
DR   OrthoDB; 508327at2759; -.
DR   PhylomeDB; Q9LSY4; -.
DR   PRO; PR:Q9LSY4; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LSY4; baseline and differential.
DR   Genevisible; Q9LSY4; AT.
DR   GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IDA:TAIR.
DR   GO; GO:0080046; F:quercetin 4'-O-glucosyltransferase activity; IDA:TAIR.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..480
FT                   /note="UDP-glycosyltransferase 71B8"
FT                   /id="PRO_0000409052"
FT   BINDING         281
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         348..350
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         365..373
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         387..390
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   480 AA;  53685 MW;  25141D33D8B3D5A3 CRC64;
     MNKFALVFVP FPILGHLKST AEMAKLLVEQ ETRLSISIII LPLLSGDDVS ASAYISALSA
     ASNDRLHYEV ISDGDQPTVG LHVDNHIPMV KRTVAKLVDD YSRRPDSPRL AGLVVDMFCI
     SVIDVANEVS VPCYLFYTSN VGILALGLHI QMLFDKKEYS VSETDFEDSE VVLDVPSLTC
     PYPVKCLPYG LATKEWLPMY LNQGRRFREM KGILVNTFAE LEPYALESLH SSGDTPRAYP
     VGPLLHLENH VDGSKDEKGS DILRWLDEQP PKSVVFLCFG SIGGFNEEQA REMAIALERS
     GHRFLWSLRR ASRDIDKELP GEFKNLEEIL PEGFFDRTKD KGKVIGWAPQ VAVLAKPAIG
     GFVTHCGWNS ILESLWFGVP IAPWPLYAEQ KFNAFVMVEE LGLAVKIRKY WRGDQLVGTA
     TVIVTAEEIE RGIRCLMEQD SDVRNRVKEM SKKCHMALKD GGSSQSALKL FIQDVTKYIA