U71D1_ARATH
ID U71D1_ARATH Reviewed; 467 AA.
AC O82383;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=UDP-glycosyltransferase 71D1;
DE EC=2.4.1.-;
DE AltName: Full=Flavonol 3-O-glucosyltransferase UGT71D1;
DE EC=2.4.1.91;
GN Name=UGT71D1; OrderedLocusNames=At2g29730; ORFNames=T27A16.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
RN [6]
RP FUNCTION.
RX PubMed=15352060; DOI=10.1002/bit.20154;
RA Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.;
RT "Arabidopsis glycosyltransferases as biocatalysts in fermentation for
RT regioselective synthesis of diverse quercetin glucosides.";
RL Biotechnol. Bioeng. 87:623-631(2004).
CC -!- FUNCTION: Possesses quercetin 3-O-glucosyltransferase activity in
CC vitro. {ECO:0000269|PubMed:15352060}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a flavonol + UDP-alpha-D-glucose = a flavonol 3-O-beta-D-
CC glucoside + H(+) + UDP; Xref=Rhea:RHEA:22300, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16816, ChEBI:CHEBI:28802, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885; EC=2.4.1.91;
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC005496; AAC35239.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08298.1; -; Genomic_DNA.
DR EMBL; AY099557; AAM20409.1; -; mRNA.
DR EMBL; BT006599; AAP31943.1; -; mRNA.
DR EMBL; AY086939; AAM64503.1; -; mRNA.
DR PIR; H84699; H84699.
DR RefSeq; NP_180534.1; NM_128527.4.
DR AlphaFoldDB; O82383; -.
DR SMR; O82383; -.
DR STRING; 3702.AT2G29730.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; O82383; -.
DR PRIDE; O82383; -.
DR ProteomicsDB; 228642; -.
DR DNASU; 817523; -.
DR EnsemblPlants; AT2G29730.1; AT2G29730.1; AT2G29730.
DR GeneID; 817523; -.
DR Gramene; AT2G29730.1; AT2G29730.1; AT2G29730.
DR KEGG; ath:AT2G29730; -.
DR Araport; AT2G29730; -.
DR TAIR; locus:2060679; AT2G29730.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_3_2_1; -.
DR InParanoid; O82383; -.
DR OMA; NQDDRIR; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; O82383; -.
DR PRO; PR:O82383; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82383; baseline and differential.
DR Genevisible; O82383; AT.
DR GO; GO:0102360; F:daphnetin 3-O-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047893; F:flavonol 3-O-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102425; F:myricetin 3-O-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IDA:TAIR.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..467
FT /note="UDP-glycosyltransferase 71D1"
FT /id="PRO_0000409058"
FT BINDING 284
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 339..341
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 356..364
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 378..381
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 467 AA; 53000 MW; 57AEA8A8058CA8E7 CRC64;
MRNVELIFIP TPTVGHLVPF LEFARRLIEQ DDRIRITILL MKLQGQSHLD TYVKSIASSQ
PFVRFIDVPE LEEKPTLGST QSVEAYVYDV IERNIPLVRN IVMDILTSLA LDGVKVKGLV
VDFFCLPMID VAKDISLPFY VFLTTNSGFL AMMQYLADRH SRDTSVFVRN SEEMLSIPGF
VNPVPANVLP SALFVEDGYD AYVKLAILFT KANGILVNSS FDIEPYSVNH FLQEQNYPSV
YAVGPIFDLK AQPHPEQDLT RRDELMKWLD DQPEASVVFL CFGSMARLRG SLVKEIAHGL
ELCQYRFLWS LRKEEVTKDD LPEGFLDRVD GRGMICGWSP QVEILAHKAV GGFVSHCGWN
SIVESLWFGV PIVTWPMYAE QQLNAFLMVK ELKLAVELKL DYRVHSDEIV NANEIETAIR
YVMDTDNNVV RKRVMDISQM IQRATKNGGS SFAAIEKFIY DVIGIKP
