U74D1_ARATH
ID U74D1_ARATH Reviewed; 456 AA.
AC Q9SKC5; Q8LFR6;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=UDP-glycosyltransferase 74D1;
DE EC=2.4.1.-;
DE AltName: Full=Jasmonate glucosyltransferase 1;
DE Short=AtJGT1;
GN Name=UGT74D1; Synonyms=JGT1; OrderedLocusNames=At2g31750;
GN ORFNames=F20M17.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP TISSUE SPECIFICITY.
RA Song J.T.;
RT "Biochemical characterization of an Arabidopsis glucosyltransferase with
RT high activity toward jasmonic acid.";
RL J. Plant Biol. 48:422-428(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
CC -!- FUNCTION: Glucosyltransferase that glucosylates jasmonate (JA) and JA
CC derivatives. Also active on indole-3-acetic acid (IAA), 4-coumrate,
CC cinnamate and caffeate. {ECO:0000269|Ref.1}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=290 uM for jasmonate {ECO:0000269|Ref.1};
CC KM=540 uM for indole-3-acetic acid {ECO:0000269|Ref.1};
CC pH dependence:
CC Optimum pH is 7.0. {ECO:0000269|Ref.1};
CC -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; DQ158907; ABA39729.1; -; mRNA.
DR EMBL; AC006533; AAD32297.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08580.1; -; Genomic_DNA.
DR EMBL; AY125506; AAM78098.1; -; mRNA.
DR EMBL; BT000622; AAN38705.1; -; mRNA.
DR EMBL; AY084687; AAM61249.1; -; mRNA.
DR PIR; F84724; F84724.
DR RefSeq; NP_180734.1; NM_128733.5.
DR AlphaFoldDB; Q9SKC5; -.
DR SMR; Q9SKC5; -.
DR BioGRID; 3079; 1.
DR STRING; 3702.AT2G31750.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; Q9SKC5; -.
DR PRIDE; Q9SKC5; -.
DR ProteomicsDB; 228675; -.
DR EnsemblPlants; AT2G31750.1; AT2G31750.1; AT2G31750.
DR GeneID; 817732; -.
DR Gramene; AT2G31750.1; AT2G31750.1; AT2G31750.
DR KEGG; ath:AT2G31750; -.
DR Araport; AT2G31750; -.
DR TAIR; locus:2045238; AT2G31750.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_0_1_1; -.
DR InParanoid; Q9SKC5; -.
DR OMA; EDVWNVG; -.
DR PhylomeDB; Q9SKC5; -.
DR BioCyc; ARA:AT2G31750-MON; -.
DR PRO; PR:Q9SKC5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SKC5; differential.
DR Genevisible; Q9SKC5; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005777; C:peroxisome; HDA:TAIR.
DR GO; GO:0052638; F:indole-3-butyrate beta-glucosyltransferase activity; IDA:TAIR.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0080044; F:quercetin 7-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0009850; P:auxin metabolic process; IDA:TAIR.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR Pfam; PF00201; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..456
FT /note="UDP-glycosyltransferase 74D1"
FT /id="PRO_0000409101"
FT BINDING 279
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 332..334
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 349..357
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 371..374
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT CONFLICT 323..324
FT /note="CD -> GE (in Ref. 5; AAM61249)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 456 AA; 51053 MW; 3DA0DAC9DB8D69A8 CRC64;
MGEKAKANVL VFSFPIQGHI NPLLQFSKRL LSKNVNVTFL TTSSTHNSIL RRAITGGATA
LPLSFVPIDD GFEEDHPSTD TSPDYFAKFQ ENVSRSLSEL ISSMDPKPNA VVYDSCLPYV
LDVCRKHPGV AAASFFTQSS TVNATYIHFL RGEFKEFQND VVLPAMPPLK GNDLPVFLYD
NNLCRPLFEL ISSQFVNVDD IDFFLVNSFD ELEVEVLQWM KNQWPVKNIG PMIPSMYLDK
RLAGDKDYGI NLFNAQVNEC LDWLDSKPPG SVIYVSFGSL AVLKDDQMIE VAAGLKQTGH
NFLWVVRETE TKKLPSNYIE DICDKGLIVN WSPQLQVLAH KSIGCFMTHC GWNSTLEALS
LGVALIGMPA YSDQPTNAKF IEDVWKVGVR VKADQNGFVP KEEIVRCVGE VMEDMSEKGK
EIRKNARRLM EFAREALSDG GNSDKNIDEF VAKIVR
