U75C1_SOLLC
ID U75C1_SOLLC Reviewed; 470 AA.
AC K4CWS6;
DT 07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=UDP-glycosyltransferase 75C1 {ECO:0000303|PubMed:28482127};
DE Short=Abscisic acid beta-glucosyltransferase {ECO:0000305};
DE Short=Indole-3-acetate beta-glucosyltransferase {ECO:0000305};
DE Short=SlUGT75C1 {ECO:0000303|PubMed:28482127};
DE EC=2.4.1.121 {ECO:0000269|PubMed:28482127};
DE EC=2.4.1.263 {ECO:0000269|PubMed:28482127};
GN Name=UGT75C1 {ECO:0000303|PubMed:28482127};
GN OrderedLocusNames=Solyc09g092500.1.1 {ECO:0000305};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Heinz 1706;
RX PubMed=22660326; DOI=10.1038/nature11119;
RG Tomato Genome Consortium;
RT "The tomato genome sequence provides insights into fleshy fruit
RT evolution.";
RL Nature 485:635-641(2012).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL
RP PROPERTIES, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND INDUCTION BY ABSCISIC ACID.
RC STRAIN=cv. MicroTom;
RX PubMed=28482127; DOI=10.1111/tpj.13588;
RA Sun Y., Ji K., Liang B., Du Y., Jiang L., Wang J., Kai W., Zhang Y.,
RA Zhai X., Chen P., Wang H., Leng P.;
RT "Suppressing ABA uridine diphosphate glucosyltransferase (SlUGT75C1) alters
RT fruit ripening and the stress response in tomato.";
RL Plant J. 91:574-589(2017).
CC -!- FUNCTION: Glucosyltransferase acting on both abscisic acid (ABA) and
CC auxin (IAA). Required for ABA-mediated fruit ripening, seed
CC germination, and negative responses to drought.
CC {ECO:0000269|PubMed:28482127}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-cis-(+)-abscisate + UDP-alpha-D-glucose = beta-D-
CC glucopyranosyl cis-(+)-abscisate + UDP; Xref=Rhea:RHEA:31031,
CC ChEBI:CHEBI:22151, ChEBI:CHEBI:37569, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885; EC=2.4.1.263;
CC Evidence={ECO:0000269|PubMed:28482127};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(indol-3-yl)acetate + UDP-alpha-D-glucose = 1-O-(indol-3-
CC ylacetyl)-beta-D-glucose + UDP; Xref=Rhea:RHEA:14921,
CC ChEBI:CHEBI:17990, ChEBI:CHEBI:30854, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885; EC=2.4.1.121;
CC Evidence={ECO:0000269|PubMed:28482127};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.27 mM for abscisic acid {ECO:0000269|PubMed:28482127};
CC Vmax=0.026 umol/min/ug enzyme with abscisic acid as substrate (in the
CC presence of UDP-glucose) {ECO:0000269|PubMed:28482127};
CC pH dependence:
CC Optimum pH is 3.67-8. {ECO:0000269|PubMed:28482127};
CC Temperature dependence:
CC Optimum temperature is 37 degrees Celsius.
CC {ECO:0000269|PubMed:28482127};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:28482127}. Nucleus
CC {ECO:0000269|PubMed:28482127}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers and fruits, especially in
CC pulp, and, at lower levels, in seeds. {ECO:0000269|PubMed:28482127}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed during fruit ripening and flower
CC development. In flowers, present at low levels during early
CC developmental stages and later accumulates rapidly to reach a peak at
CC the pre-blooming stage. Highly expressed in the sporogenous cells in
CC the anther, pistil stigma and ovule and in basal vascular bundles. In
CC fruits, accumulates progressively in the pulp with a maximum level at
CC the pink stage. Highly expressed in the seeds, fruit peel and vascular
CC tissues. {ECO:0000269|PubMed:28482127}.
CC -!- INDUCTION: Induced by abscisic acid (ABA).
CC {ECO:0000269|PubMed:28482127}.
CC -!- DISRUPTION PHENOTYPE: Increased sensitivity to exogenous abscisic acid
CC (ABA) and increased resistance to drought stress due to smaller
CC stomatal apertures. Acceleration of fruit ripening with abnormally long
CC shape by enhancing ABA levels and promoting the early release of
CC ethylene (IAA) in immature fruit, and associated with altered
CC expression of fruit ripening genes (e.g. ethylene biosynthesis and cell
CC wall catabolism). Up-regulation of the expression of CYP707A2, which
CC encodes an ABA 8'-hydroxylase, thus preventing excessive ABA
CC accumulation. Seeds exhibit delayed germination and root growth.
CC {ECO:0000269|PubMed:28482127}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; CM001072; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_004247894.1; XM_004247846.3.
DR AlphaFoldDB; K4CWS6; -.
DR SMR; K4CWS6; -.
DR STRING; 4081.Solyc09g092500.1.1; -.
DR PaxDb; K4CWS6; -.
DR PRIDE; K4CWS6; -.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_0_1_1; -.
DR InParanoid; K4CWS6; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; K4CWS6; -.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; K4CWS6; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0010294; F:abscisic acid glucosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0047215; F:indole-3-acetate beta-glucosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0080044; F:quercetin 7-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009835; P:fruit ripening; IMP:UniProtKB.
DR GO; GO:0080148; P:negative regulation of response to water deprivation; IMP:UniProtKB.
DR GO; GO:0009787; P:regulation of abscisic acid-activated signaling pathway; IMP:UniProtKB.
DR GO; GO:0009737; P:response to abscisic acid; IEP:UniProtKB.
DR GO; GO:0009845; P:seed germination; IMP:UniProtKB.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Abscisic acid signaling pathway; Cytoplasm; Developmental protein;
KW Glycosyltransferase; Nucleus; Reference proteome; Transferase.
FT CHAIN 1..470
FT /note="UDP-glycosyltransferase 75C1"
FT /id="PRO_0000445697"
FT BINDING 290
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 344..345
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 362..370
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 384..387
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
SQ SEQUENCE 470 AA; 52739 MW; 74B74E60B55B9896 CRC64;
MVQPHVLLVT FPAQGHINPS LQFAKRLIEM GIEVTFTTSV FAHRRMAKIA ASTAPKGLNL
AAFSDGFDDG FKSNVDDSKR YMSEIRSRGS QTLRDVILKS SDEGRPVTSL VYTLLLPWAA
EVARELHIPS ALLWIQPATV LDIYYYYFNG YEDEMKCSSS NDPNWSIQLP RLPLLKSQDL
PSFLVSSSSK DDKYSFALPT FKEQLDTLDG EENPKVLVNT FDALELEPLK AIEKYNLIGI
GPLIPSSFLG GKDSLESSFG GDLFQKSNDD YMEWLNTKPK SSIVYISFGS LLNLSRNQKE
EIAKGLIEIQ RPFLWVIRDQ EEEKEEEKLS CMMELEKQGK IVPWCSQLEV LTHPSLGCFV
SHCGWNSTLE SLSSGVPVVA FPHWTDQGTN AKLIEDVWKT GVRMRVNEDG VVESDEIKRC
IEIVMDGGEK GEEMRKNAQK WKELARAAVK EGGSSEVNLK AFVLQVSKSC
