ID   U763_DORVU              Reviewed;          36 AA.
AC   P0DUT1;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   23-FEB-2022, entry version 2.
DE   RecName: Full=U-limacoditoxin(7)-Dv63 {ECO:0000303|PubMed:33893140};
DE            Short=U-LCTX(7)-Dv63 {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Cecropin-like peptide {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Vulnericin {ECO:0000303|PubMed:33893140};
DE   Flags: Precursor;
OS   Doratifera vulnerans (Mottled cup moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Zygaenoidea;
OC   Limacodidae; Doratifera.
OX   NCBI_TaxID=1372962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-36, FUNCTION,
RP   SUBCELLULAR LOCATION, TOXIC DOSE, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=33893140; DOI=10.1073/pnas.2023815118;
RA   Walker A.A., Robinson S.D., Paluzzi J.V., Merritt D.J., Nixon S.A.,
RA   Schroeder C.I., Jin J., Goudarzi M.H., Kotze A.C., Dekan Z., Sombke A.,
RA   Alewood P.F., Fry B.G., Epstein M.E., Vetter I., King G.F.;
RT   "Production, composition, and mode of action of the painful defensive venom
RT   produced by a limacodid caterpillar, Doratifera vulnerans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC   -!- FUNCTION: Peptide with insecticidal and antiparasitic activities.
CC       Induces irreversible paralysis in D.melanogaster when tested at high
CC       doses. It shows a moderate antiparasitic activity against the major
CC       pathogenic nematode of ruminants (H.contortus, EC(50)=41.3 uM). Does
CC       not show antimicrobial activities. Does not induce increase in
CC       intracellular calcium in mouse DRG neurons, suggesting that it does not
CC       induce pain. {ECO:0000269|PubMed:33893140}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33893140}.
CC   -!- TISSUE SPECIFICITY: Expressed by the spine venom secretory cell. The
CC       spine is a cuticular structure containing at its base a single large
CC       nucleated venom secretory cell, as well as a central venom reservoir
CC       extending throughout the spine. It is an independent unit capable of
CC       producing, storing, and injecting venom. Spines are grouped by 50 to
CC       100 in each of the eight venom scoli on the back of D.vulnerans
CC       caterpillars. {ECO:0000269|PubMed:33893140}.
CC   -!- DEVELOPMENTAL STAGE: Only secreted by caterpillars. Adult moth do not
CC       have spines. {ECO:0000269|PubMed:33893140}.
CC   -!- MASS SPECTROMETRY: Mass=2601.4; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:33893140};
CC   -!- TOXIC DOSE: PD(50) is 10.27 nmol/g when injected into D.melanogaster.
CC       {ECO:0000269|PubMed:33893140}.
CC   -!- SIMILARITY: Belongs to the limacoditoxin-7 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT   PEPTIDE         20..36
FT                   /note="U-limacoditoxin(7)-Dv63"
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT                   /id="PRO_0000453407"
SQ   SEQUENCE   36 AA;  4213 MW;  123466995CE80F3B CRC64;
     MFKPRVILLI TIIAVFSEFK KAFKGLFEKA ANKYLH