ID   U76E1_ARATH             Reviewed;         453 AA.
AC   Q9LTH3;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=UDP-glycosyltransferase 76E1;
DE            EC=2.4.1.-;
GN   Name=UGT76E1; OrderedLocusNames=At5g59580; ORFNames=F2O15.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA   Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT   "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT   Arabidopsis thaliana.";
RL   J. Biol. Chem. 276:4338-4343(2001).
RN   [4]
RP   FUNCTION.
RX   PubMed=15352060; DOI=10.1002/bit.20154;
RA   Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.;
RT   "Arabidopsis glycosyltransferases as biocatalysts in fermentation for
RT   regioselective synthesis of diverse quercetin glucosides.";
RL   Biotechnol. Bioeng. 87:623-631(2004).
CC   -!- FUNCTION: Possesses low quercetin 3-O-glucosyltransferase and 7-O-
CC       glucosyltransferase activities in vitro. {ECO:0000269|PubMed:15352060}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AB025604; BAA97492.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97208.1; -; Genomic_DNA.
DR   RefSeq; NP_200766.2; NM_125350.3.
DR   AlphaFoldDB; Q9LTH3; -.
DR   SMR; Q9LTH3; -.
DR   STRING; 3702.AT5G59580.1; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   iPTMnet; Q9LTH3; -.
DR   PaxDb; Q9LTH3; -.
DR   PRIDE; Q9LTH3; -.
DR   ProteomicsDB; 228680; -.
DR   EnsemblPlants; AT5G59580.1; AT5G59580.1; AT5G59580.
DR   GeneID; 836077; -.
DR   Gramene; AT5G59580.1; AT5G59580.1; AT5G59580.
DR   KEGG; ath:AT5G59580; -.
DR   Araport; AT5G59580; -.
DR   TAIR; locus:2148363; AT5G59580.
DR   eggNOG; KOG1192; Eukaryota.
DR   HOGENOM; CLU_001724_0_0_1; -.
DR   InParanoid; Q9LTH3; -.
DR   OMA; KLMANGH; -.
DR   OrthoDB; 508327at2759; -.
DR   PhylomeDB; Q9LTH3; -.
DR   PRO; PR:Q9LTH3; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LTH3; baseline and differential.
DR   Genevisible; Q9LTH3; AT.
DR   GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IDA:TAIR.
DR   GO; GO:0080044; F:quercetin 7-O-glucosyltransferase activity; IDA:TAIR.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   Pfam; PF00201; UDPGT; 1.
PE   2: Evidence at transcript level;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..453
FT                   /note="UDP-glycosyltransferase 76E1"
FT                   /id="PRO_0000409086"
FT   BINDING         272
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         331..333
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         348..356
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         370..373
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   453 AA;  50804 MW;  E38AC3DC542841D3 CRC64;
     MEELGVKRRI VLVPVPAQGH VTPIMQLGKA LYSKGFSITV VLTQYNRVSS SKDFSDFHFL
     TIPGSLTESD LKNLGPFKFL FKLNQICEAS FKQCIGQLLQ EQGNDIACVV YDEYMYFSQA
     AVKEFQLPSV LFSTTSATAF VCRSVLSRVN AESFLLDMKD PKVSDKEFPG LHPLRYKDLP
     TSAFGPLESI LKVYSETVNI RTASAVIINS TSCLESSSLA WLQKQLQVPV YPIGPLHIAA
     SAPSSLLEED RSCLEWLNKQ KIGSVIYISL GSLALMETKD MLEMAWGLRN SNQPFLWVIR
     PGSIPGSEWT ESLPEEFSRL VSERGYIVKW APQIEVLRHP AVGGFWSHCG WNSTLESIGE
     GVPMICRPFT GDQKVNARYL ERVWRIGVQL EGELDKGTVE RAVERLIMDE EGAEMRKRVI
     NLKEKLQASV KSRGSSFSSL DNFVNSLKMM NFM