U76E2_ARATH
ID U76E2_ARATH Reviewed; 449 AA.
AC Q9LTH2;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=UDP-glycosyltransferase 76E2;
DE EC=2.4.1.-;
GN Name=UGT76E2; OrderedLocusNames=At5g59590; ORFNames=F2O15.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
RN [5]
RP FUNCTION.
RX PubMed=15352060; DOI=10.1002/bit.20154;
RA Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.;
RT "Arabidopsis glycosyltransferases as biocatalysts in fermentation for
RT regioselective synthesis of diverse quercetin glucosides.";
RL Biotechnol. Bioeng. 87:623-631(2004).
CC -!- FUNCTION: Possesses low quercetin 3-O-glucosyltransferase and 7-O-
CC glucosyltransferase activities in vitro. {ECO:0000269|PubMed:15352060}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB025604; BAA97493.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97209.1; -; Genomic_DNA.
DR EMBL; BT004159; AAO42179.1; -; mRNA.
DR EMBL; BT005494; AAO63914.1; -; mRNA.
DR RefSeq; NP_200767.1; NM_125351.3.
DR AlphaFoldDB; Q9LTH2; -.
DR SMR; Q9LTH2; -.
DR STRING; 3702.AT5G59590.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; Q9LTH2; -.
DR PRIDE; Q9LTH2; -.
DR ProteomicsDB; 228648; -.
DR EnsemblPlants; AT5G59590.1; AT5G59590.1; AT5G59590.
DR GeneID; 836078; -.
DR Gramene; AT5G59590.1; AT5G59590.1; AT5G59590.
DR KEGG; ath:AT5G59590; -.
DR Araport; AT5G59590; -.
DR TAIR; locus:2148378; AT5G59590.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_0_0_1; -.
DR InParanoid; Q9LTH2; -.
DR OMA; ITIAHTS; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; Q9LTH2; -.
DR PRO; PR:Q9LTH2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LTH2; baseline and differential.
DR Genevisible; Q9LTH2; AT.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IDA:TAIR.
DR GO; GO:0080044; F:quercetin 7-O-glucosyltransferase activity; IDA:TAIR.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR Pfam; PF00201; UDPGT; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..449
FT /note="UDP-glycosyltransferase 76E2"
FT /id="PRO_0000409087"
FT BINDING 274
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 333..335
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 350..358
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 372..375
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 449 AA; 50096 MW; 491589059CB2E88B CRC64;
MEEKQVKETR IVLVPVPAQG HVTPMMQLGK ALHSKGFSIT VVLTQSNRVS SSKDFSDFHF
LTIPGSLTES DLQNLGPQKF VLKLNQICEA SFKQCIGQLL HEQCNNDIAC VVYDEYMYFS
HAAVKEFQLP SVVFSTTSAT AFVCRSVLSR VNAESFLIDM KDPETQDKVF PGLHPLRYKD
LPTSVFGPIE STLKVYSETV NTRTASAVII NSASCLESSS LARLQQQLQV PVYPIGPLHI
TASAPSSLLE EDRSCVEWLN KQKSNSVIYI SLGSLALMDT KDMLEMAWGL SNSNQPFLWV
VRPGSIPGSE WTESLPEEFN RLVSERGYIV KWAPQMEVLR HPAVGGFWSH CGWNSTVESI
GEGVPMICRP FTGDQKVNAR YLERVWRIGV QLEGDLDKET VERAVEWLLV DEEGAEMRKR
AIDLKEKIET SVRSGGSSCS SLDDFVNSM
