U80B1_ARATH
ID U80B1_ARATH Reviewed; 615 AA.
AC Q9XIG1;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Sterol 3-beta-glucosyltransferase UGT80B1 {ECO:0000305};
DE EC=2.4.1.173 {ECO:0000269|PubMed:19641030};
DE AltName: Full=Protein TRANSPARENT TESTA 15 {ECO:0000303|PubMed:19641030};
DE AltName: Full=UDP-glucose:sterol glucosyltransferase 80B1 {ECO:0000303|PubMed:19641030};
GN Name=UGT80B1 {ECO:0000303|PubMed:19641030};
GN Synonyms=TT15 {ECO:0000303|PubMed:19641030};
GN OrderedLocusNames=At1g43620 {ECO:0000312|Araport:AT1G43620};
GN ORFNames=T10P12.7 {ECO:0000312|EMBL:AAD39269.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19641030; DOI=10.1104/pp.109.140582;
RA DeBolt S., Scheible W.R., Schrick K., Auer M., Beisson F., Bischoff V.,
RA Bouvier-Nave P., Carroll A., Hematy K., Li Y., Milne J., Nair M.,
RA Schaller H., Zemla M., Somerville C.;
RT "Mutations in UDP-Glucose:sterol glucosyltransferase in Arabidopsis cause
RT transparent testa phenotype and suberization defect in seeds.";
RL Plant Physiol. 151:78-87(2009).
RN [6]
RP FUNCTION.
RX PubMed=21830156; DOI=10.1007/s11745-011-3602-9;
RA Schrick K., Shiva S., Arpin J.C., Delimont N., Isaac G., Tamura P.,
RA Welti R.;
RT "Steryl glucoside and acyl steryl glucoside analysis of Arabidopsis seeds
RT by electrospray ionization tandem mass spectrometry.";
RL Lipids 47:185-193(2012).
CC -!- FUNCTION: Involved in the biosynthesis of sterol glucosides. Catalyzes
CC the synthesis of steryl glycosides (SGs) and acyl steryl glycosides
CC (ASGs) which are the most abundant sterol derivatives in higher plants.
CC Can act on several sterols like sitosterol, campesterol and
CC stigmasterol. Is required for embryonic development, seed suberin
CC accumulation, cutin formation and flavanoid accumulation in the seed
CC coat. Both UGT80A2 and UGT80B1 are required for the normal production
CC of SGs and ASGs in seeds. {ECO:0000269|PubMed:19641030,
CC ECO:0000269|PubMed:21830156}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside +
CC H(+) + UDP; Xref=Rhea:RHEA:22724, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15889, ChEBI:CHEBI:37424, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885; EC=2.4.1.173;
CC Evidence={ECO:0000269|PubMed:19641030};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22725;
CC Evidence={ECO:0000269|PubMed:19641030};
CC -!- TISSUE SPECIFICITY: Expressed in developing seeds, seedlings, leaves
CC and around the apical tip of cotyledons. In embryo, expressed in the
CC seed coat and cotyledons. {ECO:0000269|PubMed:19641030}.
CC -!- DISRUPTION PHENOTYPE: Reduced growth rates. Altered embryonic
CC development, seed suberin accumulation, cutin formation in the seed
CC coat and reduced seed size. Lack of flavanoid accumulation in the seed
CC coat (transparent testa phenotype). {ECO:0000269|PubMed:19641030}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC007203; AAD39269.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31974.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31975.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31976.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58456.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58458.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58459.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58460.1; -; Genomic_DNA.
DR EMBL; BT005834; AAO64769.1; -; mRNA.
DR EMBL; AK227397; BAE99401.1; -; mRNA.
DR PIR; D96499; D96499.
DR RefSeq; NP_001077674.1; NM_001084205.2.
DR RefSeq; NP_001319161.1; NM_001333213.1.
DR RefSeq; NP_001320888.1; NM_001333215.1.
DR RefSeq; NP_001320890.1; NM_001333214.1.
DR RefSeq; NP_001320891.1; NM_001333216.1.
DR RefSeq; NP_175027.1; NM_103487.4.
DR RefSeq; NP_973967.1; NM_202238.3.
DR AlphaFoldDB; Q9XIG1; -.
DR SMR; Q9XIG1; -.
DR STRING; 3702.AT1G43620.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR iPTMnet; Q9XIG1; -.
DR SwissPalm; Q9XIG1; -.
DR PaxDb; Q9XIG1; -.
DR PRIDE; Q9XIG1; -.
DR ProteomicsDB; 228678; -.
DR EnsemblPlants; AT1G43620.1; AT1G43620.1; AT1G43620.
DR EnsemblPlants; AT1G43620.2; AT1G43620.2; AT1G43620.
DR EnsemblPlants; AT1G43620.3; AT1G43620.3; AT1G43620.
DR EnsemblPlants; AT1G43620.4; AT1G43620.4; AT1G43620.
DR EnsemblPlants; AT1G43620.5; AT1G43620.5; AT1G43620.
DR EnsemblPlants; AT1G43620.6; AT1G43620.6; AT1G43620.
DR EnsemblPlants; AT1G43620.7; AT1G43620.7; AT1G43620.
DR GeneID; 840946; -.
DR Gramene; AT1G43620.1; AT1G43620.1; AT1G43620.
DR Gramene; AT1G43620.2; AT1G43620.2; AT1G43620.
DR Gramene; AT1G43620.3; AT1G43620.3; AT1G43620.
DR Gramene; AT1G43620.4; AT1G43620.4; AT1G43620.
DR Gramene; AT1G43620.5; AT1G43620.5; AT1G43620.
DR Gramene; AT1G43620.6; AT1G43620.6; AT1G43620.
DR Gramene; AT1G43620.7; AT1G43620.7; AT1G43620.
DR KEGG; ath:AT1G43620; -.
DR Araport; AT1G43620; -.
DR TAIR; locus:2194676; AT1G43620.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_000537_8_3_1; -.
DR InParanoid; Q9XIG1; -.
DR OMA; ICPFFGD; -.
DR OrthoDB; 1024049at2759; -.
DR PhylomeDB; Q9XIG1; -.
DR BRENDA; 2.4.1.173; 399.
DR PRO; PR:Q9XIG1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9XIG1; baseline and differential.
DR Genevisible; Q9XIG1; AT.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0102203; F:brassicasterol glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102205; F:cholesterol alpha-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102202; F:soladodine glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016906; F:sterol 3-beta-glucosyltransferase activity; IMP:TAIR.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IMP:TAIR.
DR GO; GO:0030259; P:lipid glycosylation; IEA:InterPro.
DR GO; GO:0010214; P:seed coat development; IMP:TAIR.
DR GO; GO:0009845; P:seed germination; IMP:TAIR.
DR GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0016125; P:sterol metabolic process; IMP:TAIR.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR004276; GlycoTrans_28_N.
DR InterPro; IPR002213; UDP_glucos_trans.
DR Pfam; PF03033; Glyco_transf_28; 1.
DR Pfam; PF00201; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Lipid biosynthesis; Lipid metabolism;
KW Reference proteome; Steroid biosynthesis; Steroid metabolism;
KW Sterol biosynthesis; Sterol metabolism; Transferase.
FT CHAIN 1..615
FT /note="Sterol 3-beta-glucosyltransferase UGT80B1"
FT /id="PRO_0000422075"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..54
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 615 AA; 68345 MW; 71FA718E93096E88 CRC64;
MASNVFDHPL QELEGEDNGV KSEKASLLET SGSVDTTPED SGHRSSDGHR GLDHCETAPV
GLYGDMLIND SEIQYSRSLT EKGSPAIHNL KLDRLSEQEK QKLIVELVRI QNDGTVEVID
NGTPVSELWE FEPTKGQSTI TYEKSLTESF RSIPRLKIAI LVVGTRGDVQ PFLAMAKRLQ
EFGHRVRLAT HANFRSFVRA AGVEFYPLGG DPRELAAYMA RNKGLIPSGP SEISKQRKQL
KAIIESLLPA CIEPDLETAT SFRAQAIIAN PPAYGHVHVA EALGVPIHIF FTMPWTPTNE
FPHPLARVPQ SAAYWLSYIV VDLMVWWSIR TYINDFRKRK LNLAPIAYFS TYHGSISHLP
TGYMWSPHVV PKPSDWGPLV DVVGYCFLNL GSKYQPREEF LHWIERGSPP VYIGFGSMPL
DDPKQTMDII LETLKDTEQR GIVDRGWGGL GNLATEVPEN VFLVEDCPHD WLFPQCSAVV
HHGGAGTTAT GLKAGCPTTI VPFFGDQFFW GDRIYEKGLG PAPIPIAQLS VENLSSSIRF
MLQPEVKSQV MELAKVLENE DGVAAAVDAF HRHLPPELPL PESSSEKKDE DDRPDLLQWF
FIQIGKKCCL PCGGV
