U85A1_ARATH
ID U85A1_ARATH Reviewed; 489 AA.
AC Q9SK82;
DT 24-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=UDP-glycosyltransferase 85A1;
DE EC=2.4.1.-;
DE AltName: Full=Cytokinin-O-glucosyltransferase 2;
DE AltName: Full=Zeatin O-glucosyltransferase 2;
DE Short=AtZOG2;
GN Name=UGT85A1; Synonyms=ZOG2; OrderedLocusNames=At1g22400;
GN ORFNames=F12K8.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
RN [5]
RP FUNCTION.
RX PubMed=15342621; DOI=10.1074/jbc.m409569200;
RA Hou B., Lim E.-K., Higgins G.S., Bowles D.J.;
RT "N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis
RT thaliana.";
RL J. Biol. Chem. 279:47822-47832(2004).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=17498920; DOI=10.1016/j.ygeno.2007.03.014;
RA Woo H.H., Jeong B.R., Hirsch A.M., Hawes M.C.;
RT "Characterization of Arabidopsis AtUGT85A and AtGUS gene families and their
RT expression in rapidly dividing tissues.";
RL Genomics 90:143-153(2007).
CC -!- FUNCTION: Involved in the O-glucosylation of trans-zeatin and
CC dihydrozeatin. Also active in vitro on cis-zeatin. Not active on N-
CC glucosylated substrates. {ECO:0000269|PubMed:15342621}.
CC -!- TISSUE SPECIFICITY: Expressed in root tips, lateral root initials, root
CC apex, shoots, leaf periphery, leaf primordia and flowers.
CC {ECO:0000269|PubMed:17498920}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AC006551; AAF18537.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30237.1; -; Genomic_DNA.
DR EMBL; AY081339; AAL91228.1; -; mRNA.
DR EMBL; BT008765; AAP49527.1; -; mRNA.
DR PIR; H86356; H86356.
DR RefSeq; NP_173656.1; NM_102089.5.
DR AlphaFoldDB; Q9SK82; -.
DR SMR; Q9SK82; -.
DR STRING; 3702.AT1G22400.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; Q9SK82; -.
DR PRIDE; Q9SK82; -.
DR ProteomicsDB; 242812; -.
DR EnsemblPlants; AT1G22400.1; AT1G22400.1; AT1G22400.
DR GeneID; 838846; -.
DR Gramene; AT1G22400.1; AT1G22400.1; AT1G22400.
DR KEGG; ath:AT1G22400; -.
DR Araport; AT1G22400; -.
DR TAIR; locus:2009557; AT1G22400.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_0_0_1; -.
DR InParanoid; Q9SK82; -.
DR OMA; PLICWSF; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; Q9SK82; -.
DR BioCyc; ARA:AT1G22400-MON; -.
DR BioCyc; MetaCyc:AT1G22400-MON; -.
DR BRENDA; 2.4.1.203; 399.
DR PRO; PR:Q9SK82; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SK82; baseline and differential.
DR Genevisible; Q9SK82; AT.
DR GO; GO:0050502; F:cis-zeatin O-beta-D-glucosyltransferase activity; IDA:TAIR.
DR GO; GO:0015020; F:glucuronosyltransferase activity; ISS:TAIR.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0080044; F:quercetin 7-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0050403; F:trans-zeatin O-beta-D-glucosyltransferase activity; IDA:TAIR.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..489
FT /note="UDP-glycosyltransferase 85A1"
FT /id="PRO_0000074156"
FT BINDING 307
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 364..366
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 381..389
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 403..406
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 489 AA; 55010 MW; BC44EDF1AA20FEE3 CRC64;
MGSQIIHNSQ KPHVVCVPYP AQGHINPMMR VAKLLHARGF YVTFVNTVYN HNRFLRSRGS
NALDGLPSFR FESIADGLPE TDMDATQDIT ALCESTMKNC LAPFRELLQR INAGDNVPPV
SCIVSDGCMS FTLDVAEELG VPEVLFWTTS GCAFLAYLHF YLFIEKGLCP LKDESYLTKE
YLEDTVIDFI PTMKNVKLKD IPSFIRTTNP DDVMISFALR ETERAKRASA IILNTFDDLE
HDVVHAMQSI LPPVYSVGPL HLLANREIEE GSEIGMMSSN LWKEEMECLD WLDTKTQNSV
IYINFGSITV LSVKQLVEFA WGLAGSGKEF LWVIRPDLVA GEEAMVPPDF LMETKDRSML
ASWCPQEKVL SHPAIGGFLT HCGWNSILES LSCGVPMVCW PFFADQQMNC KFCCDEWDVG
IEIGGDVKRE EVEAVVRELM DGEKGKKMRE KAVEWQRLAE KATEHKLGSS VMNFETVVSK
FLLGQKSQD
