U85A2_ARATH
ID U85A2_ARATH Reviewed; 481 AA.
AC Q9ZWJ3; C0Z2M0; Q8W4G1;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=UDP-glycosyltransferase 85A2;
DE EC=2.4.1.-;
GN Name=UGT85A2; OrderedLocusNames=At1g22360; ORFNames=T16E15.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Yamagishi E., Gond Z., Yamazaki M., Saito K.;
RT "Molecular cloning of a cDNA encoding a novel VDP-Glucose
RT glucosyltransferase homologue from Arabidopsis thaliana.";
RL (er) Plant Gene Register PGR98-187(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [6]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=17498920; DOI=10.1016/j.ygeno.2007.03.014;
RA Woo H.H., Jeong B.R., Hirsch A.M., Hawes M.C.;
RT "Characterization of Arabidopsis AtUGT85A and AtGUS gene families and their
RT expression in rapidly dividing tissues.";
RL Genomics 90:143-153(2007).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ZWJ3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZWJ3-3; Sequence=VSP_041229, VSP_041230;
CC -!- TISSUE SPECIFICITY: Expressed in roots, shoots, leaves and flowers.
CC {ECO:0000269|PubMed:17498920}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB016819; BAA34687.1; -; mRNA.
DR EMBL; AC068562; AAF87256.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30232.1; -; Genomic_DNA.
DR EMBL; AF332418; AAG48781.1; -; mRNA.
DR EMBL; AY062579; AAL32657.1; -; mRNA.
DR EMBL; AY093357; AAM13356.1; -; mRNA.
DR EMBL; AK318834; BAH56949.1; -; mRNA.
DR PIR; E86356; E86356.
DR RefSeq; NP_173653.1; NM_102086.3. [Q9ZWJ3-1]
DR AlphaFoldDB; Q9ZWJ3; -.
DR SMR; Q9ZWJ3; -.
DR BioGRID; 24082; 1.
DR STRING; 3702.AT1G22360.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR iPTMnet; Q9ZWJ3; -.
DR PaxDb; Q9ZWJ3; -.
DR PRIDE; Q9ZWJ3; -.
DR ProteomicsDB; 228734; -. [Q9ZWJ3-1]
DR EnsemblPlants; AT1G22360.1; AT1G22360.1; AT1G22360. [Q9ZWJ3-1]
DR GeneID; 838843; -.
DR Gramene; AT1G22360.1; AT1G22360.1; AT1G22360. [Q9ZWJ3-1]
DR KEGG; ath:AT1G22360; -.
DR Araport; AT1G22360; -.
DR TAIR; locus:2196501; AT1G22360.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_0_0_1; -.
DR InParanoid; Q9ZWJ3; -.
DR OMA; WREKAIM; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; Q9ZWJ3; -.
DR BioCyc; ARA:AT1G22360-MON; -.
DR PRO; PR:Q9ZWJ3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ZWJ3; baseline and differential.
DR Genevisible; Q9ZWJ3; AT.
DR GO; GO:0015020; F:glucuronosyltransferase activity; ISS:TAIR.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0080044; F:quercetin 7-O-glucosyltransferase activity; IBA:GO_Central.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..481
FT /note="UDP-glycosyltransferase 85A2"
FT /id="PRO_0000409126"
FT BINDING 303
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 360..362
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 377..385
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 399..402
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT VAR_SEQ 425..427
FT /note="REE -> SEE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_041229"
FT VAR_SEQ 428..481
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_041230"
FT CONFLICT 191
FT /note="N -> D (in Ref. 4; AAL32657/AAM13356)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="D -> N (in Ref. 4; AAL32657/AAM13356)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 481 AA; 54364 MW; 6B96A6314AE1C324 CRC64;
MGSHVAQKQH VVCVPYPAQG HINPMMKVAK LLYAKGFHIT FVNTVYNHNR LLRSRGPNAV
DGLPSFRFES IPDGLPETDV DVTQDIPTLC ESTMKHCLAP FKELLRQINA RDDVPPVSCI
VSDGCMSFTL DAAEELGVPE VLFWTTSACG FLAYLYYYRF IEKGLSPIKD ESYLTKEHLD
TKIDWIPSMK NLRLKDIPSF IRTTNPDDIM LNFIIREADR AKRASAIILN TFDDLEHDVI
QSMKSIVPPV YSIGPLHLLE KQESGEYSEI GRTGSNLWRE ETECLDWLNT KARNSVVYVN
FGSITVLSAK QLVEFAWGLA ATGKEFLWVI RPDLVAGDEA MVPPEFLTAT ADRRMLASWC
PQEKVLSHPA IGGFLTHCGW NSTLESLCGG VPMVCWPFFA EQQTNCKFSR DEWEVGIEIG
GDVKREEVEA VVRELMDEEK GKNMREKAEE WRRLANEATE HKHGSSKLNF EMLVNKVLLG
E