U85A5_ARATH
ID U85A5_ARATH Reviewed; 479 AA.
AC Q9LMF0; Q0WL30; Q5UB81; Q94BU0;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=UDP-glycosyltransferase 85A5;
DE EC=2.4.1.-;
GN Name=UGT85A5; Synonyms=UGT3; OrderedLocusNames=At1g22370;
GN ORFNames=T16E15.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Woo H.-H.;
RT "Control of Arabidopsis development by glucuronide metabolism.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 270-479.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=17498920; DOI=10.1016/j.ygeno.2007.03.014;
RA Woo H.H., Jeong B.R., Hirsch A.M., Hawes M.C.;
RT "Characterization of Arabidopsis AtUGT85A and AtGUS gene families and their
RT expression in rapidly dividing tissues.";
RL Genomics 90:143-153(2007).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9LMF0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LMF0-2; Sequence=VSP_041231;
CC -!- TISSUE SPECIFICITY: Expressed in roots, shoots and leaves.
CC {ECO:0000269|PubMed:17498920}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AY765462; AAV32497.1; -; mRNA.
DR EMBL; AC068562; AAF87255.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30234.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30235.1; -; Genomic_DNA.
DR EMBL; AY039897; AAK64001.1; -; mRNA.
DR EMBL; AY077671; AAL76149.1; -; mRNA.
DR EMBL; AK230378; BAF02177.1; -; mRNA.
DR PIR; F86356; F86356.
DR RefSeq; NP_564170.1; NM_102087.2. [Q9LMF0-2]
DR RefSeq; NP_973885.1; NM_202156.2. [Q9LMF0-1]
DR AlphaFoldDB; Q9LMF0; -.
DR SMR; Q9LMF0; -.
DR STRING; 3702.AT1G22370.2; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR iPTMnet; Q9LMF0; -.
DR PaxDb; Q9LMF0; -.
DR PRIDE; Q9LMF0; -.
DR ProteomicsDB; 228713; -. [Q9LMF0-1]
DR EnsemblPlants; AT1G22370.1; AT1G22370.1; AT1G22370. [Q9LMF0-2]
DR EnsemblPlants; AT1G22370.2; AT1G22370.2; AT1G22370. [Q9LMF0-1]
DR GeneID; 838844; -.
DR Gramene; AT1G22370.1; AT1G22370.1; AT1G22370. [Q9LMF0-2]
DR Gramene; AT1G22370.2; AT1G22370.2; AT1G22370. [Q9LMF0-1]
DR KEGG; ath:AT1G22370; -.
DR Araport; AT1G22370; -.
DR TAIR; locus:2196496; AT1G22370.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_0_0_1; -.
DR InParanoid; Q9LMF0; -.
DR OMA; LWREESE; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; Q9LMF0; -.
DR BioCyc; ARA:AT1G22370-MON; -.
DR PRO; PR:Q9LMF0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LMF0; baseline and differential.
DR Genevisible; Q9LMF0; AT.
DR GO; GO:0015020; F:glucuronosyltransferase activity; ISS:TAIR.
DR GO; GO:0080043; F:quercetin 3-O-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0080044; F:quercetin 7-O-glucosyltransferase activity; IBA:GO_Central.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..479
FT /note="UDP-glycosyltransferase 85A5"
FT /id="PRO_0000409129"
FT BINDING 301
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 358..360
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 375..383
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 397..400
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..172
FT /note="MASHAVTSGQKPHVVCIPFPAQGHINPMLKVAKLLYARGFHVTFVNTNYNHN
FT RLIRSRGPNSLDGLPSFRFESIPDGLPEENKDVMQDVPTLCESTMKNCLAPFKELLRRI
FT NTTKDVPPVSCIVSDGVMSFTLDAAEELGVPDVLFWTPSACGFLAYLHFYRFIEKGLSP
FT IK -> MA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_041231"
FT CONFLICT 172
FT /note="K -> KGIMA (in Ref. 1; AAV32497)"
FT /evidence="ECO:0000305"
FT CONFLICT 448
FT /note="E -> G (in Ref. 5; BAF02177)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 479 AA; 53970 MW; AC836BF062A45703 CRC64;
MASHAVTSGQ KPHVVCIPFP AQGHINPMLK VAKLLYARGF HVTFVNTNYN HNRLIRSRGP
NSLDGLPSFR FESIPDGLPE ENKDVMQDVP TLCESTMKNC LAPFKELLRR INTTKDVPPV
SCIVSDGVMS FTLDAAEELG VPDVLFWTPS ACGFLAYLHF YRFIEKGLSP IKDESSLDTK
INWIPSMKNL GLKDIPSFIR ATNTEDIMLN FFVHEADRAK RASAIILNTF DSLEHDVVRS
IQSIIPQVYT IGPLHLFVNR DIDEESDIGQ IGTNMWREEM ECLDWLDTKS PNSVVYVNFG
SITVMSAKQL VEFAWGLAAT KKDFLWVIRP DLVAGDVPML PPDFLIETAN RRMLASWCPQ
EKVLSHPAVG GFLTHSGWNS TLESLSGGVP MVCWPFFAEQ QTNCKYCCDE WEVGMEIGGD
VRREEVEELV RELMDGDKGK KMRQKAEEWQ RLAEEATKPI YGSSELNFQM VVDKVLLGE
