ID   UAFA_STAS1              Reviewed;        2316 AA.
AC   Q4A0V8;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Uro-adherence factor A;
DE   Flags: Precursor;
GN   Name=uafA; OrderedLocusNames=SSP0135;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- FUNCTION: Involved in adherence to the cells of the urinary tract.
CC       Shows hemagglutination with sheep erythrocytes as well as good
CC       adherence to human T24 bladder carcinoma cells when overexpressed in a
CC       strain with non-hemagglutination. {ECO:0000269|PubMed:16135568}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}; Peptidoglycan-
CC       anchor {ECO:0000305}.
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DR   EMBL; AP008934; BAE17280.1; -; Genomic_DNA.
DR   PDB; 3IRP; X-ray; 1.50 A; X=393-811.
DR   PDB; 3IRZ; X-ray; 1.70 A; A=393-811.
DR   PDB; 3IS0; X-ray; 1.75 A; X=393-811.
DR   PDB; 3IS1; X-ray; 2.45 A; X=376-811.
DR   PDBsum; 3IRP; -.
DR   PDBsum; 3IRZ; -.
DR   PDBsum; 3IS0; -.
DR   PDBsum; 3IS1; -.
DR   AlphaFoldDB; Q4A0V8; -.
DR   SMR; Q4A0V8; -.
DR   STRING; 342451.SSP0135; -.
DR   EnsemblBacteria; BAE17280; BAE17280; SSP0135.
DR   KEGG; ssp:SSP0135; -.
DR   eggNOG; COG4932; Bacteria.
DR   eggNOG; COG5295; Bacteria.
DR   HOGENOM; CLU_230008_0_0_9; -.
DR   OMA; ESQGLME; -.
DR   EvolutionaryTrace; Q4A0V8; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 2.60.40.1280; -; 1.
DR   InterPro; IPR008966; Adhesion_dom_sf.
DR   InterPro; IPR011252; Fibrogen-bd_dom1.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR019931; LPXTG_anchor.
DR   InterPro; IPR033764; Sdr_B.
DR   InterPro; IPR041171; SDR_Ig.
DR   InterPro; IPR005877; YSIRK_signal_dom.
DR   Pfam; PF17961; Big_8; 1.
DR   Pfam; PF17210; SdrD_B; 1.
DR   Pfam; PF04650; YSIRK_signal; 1.
DR   SUPFAM; SSF49401; SSF49401; 2.
DR   TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR   PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell wall; Hemagglutinin; Peptidoglycan-anchor;
KW   Reference proteome; Repeat; Secreted; Signal; Virulence.
FT   SIGNAL          1..50
FT                   /evidence="ECO:0000255"
FT   CHAIN           51..2283
FT                   /note="Uro-adherence factor A"
FT                   /id="PRO_0000042835"
FT   PROPEP          2284..2316
FT                   /note="Removed by sortase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT                   /id="PRO_0000042836"
FT   REGION          50..262
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          861..2292
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           2280..2284
FT                   /note="LPXTG sorting signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT   COMPBIAS        55..73
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        74..88
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        98..240
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        241..262
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        861..2267
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        2268..2286
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         2283
FT                   /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT   HELIX           399..401
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          402..412
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   HELIX           415..417
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          419..421
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   TURN            422..425
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          427..436
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          445..449
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   TURN            451..453
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          454..462
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          475..482
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   TURN            483..486
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          487..492
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   HELIX           495..498
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          503..512
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   TURN            514..516
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          522..530
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          533..542
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          547..557
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          559..568
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          575..588
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   HELIX           592..594
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          595..602
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   HELIX           603..605
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   HELIX           614..616
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          617..622
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          624..627
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          633..638
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          644..649
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          658..670
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          676..688
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   HELIX           689..691
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          695..704
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          708..712
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          723..727
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          733..738
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          743..759
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          765..767
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   TURN            775..777
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          782..784
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          787..796
FT                   /evidence="ECO:0007829|PDB:3IRP"
FT   STRAND          804..808
FT                   /evidence="ECO:0007829|PDB:3IRP"
SQ   SEQUENCE   2316 AA;  245870 MW;  22F8EC8E4F17B5F4 CRC64;
     MNKNKKRHRF DFLPNRLNKY SIRKFTNGIA SVLIGSTILL GAVIDKEADA AEQQPTSEVY
     GQTDNNYKSE SNKSNSVQHD EERTNIINDN DEANYSNHSE IPIHDKSSEY DQKPINEQDT
     SNHHETHLNQ NATENHVKEE SKEVSTEEES IEDRKTEEST TEESKAVEEA NKENTTEKND
     EGSLDLEKEK DTYKDEKDNG KKKNELESHN HRIENKVEDN VYKNNKETNL ESKNENVNKD
     DKVNTSSSTS IEKPEDNATR SNLINSVNHS LKQLDNAKNN TEKQSLLENY YQTHTNATAS
     DAKKAIEKLN IDFTKQNSDQ LIALLLIELA NQMDKDKVQA NVPASKRAET NNESLSIETN
     TTNIEKTLAK PSTSKFRSAN TRATNVVNYA ANQSGRNVNH LVFANTSYEI LGGGKKYNQV
     FMTMDGKLKI KIDYTVDDSV VEGDYFTVDF GKYIHPGTSR KPYRVNNIHD ANGRTIAIGS
     YDSATNTAKY TFTNYVDIYN NVRGSFSLLS WPFKELVTTD KQSVPVGITV AGEDYTQNVI
     FNYGNRTVPV ISDINYLTKD FAEFTTYINQ NRAFNTGSKV RLSGQGFKFT SPDEIEVYKV
     LNNSQFRDSF SPDYANLTQV RNPKIIINSD GSATVDLGDI GTLGYIIRSK PNTLPDFSGI
     GVLKSEYTFT NNKNQRDTRA HASSIQFVRA ELAGFGGFGG YVWFDKNNDG VQNDSNAAAA
     GITVNLLDPT GIRLATTTTD ITGHYNFDNL TNGNYLVEFV MPEGYIPTQA NSTVDDKDSD
     VVFENGRYIA HVTIKDADNM TIDAGLVSDT TSESLSLSES LSTSQSLSLS HSLSLSESES
     TSQSLSLSTS ESLSQSLSLS ASESLSESES LSESESLSES ESLSASESLS ESESLSESES
     LSASESLSTS ESLSASESLS ESESLSESES LSESESLSES ESLSTSESLS ASESLSESES
     LSESESLSAS ESLSDSESLS ASESLSASES LSASESLSAS ESLSASESLS ESESLSDSES
     LSESESLSES ESLSTSESLS ASESISASES LSTSESLSTS ESLSESESLS ESESLSEYES
     LSASESLSAS ESLSTSESLS ESASLSESES LSTSESLSAS ESISASESLS ASESLSESES
     LSTSESLSTS ESLSESESLS ESESLSSSES LSSSESLSES ESLSTSESIS ESESLSTSES
     LSASESLSES ESLSASESIS ESESLSASES LSEYESLSTS ESLSSSESLS ESESLSASES
     LSESESLSAS ESISESESLS ESESLSTSES LSASESLSES ESLSESESLS ESESLSESES
     LSASESLSES ESLSESESLS ARESLSQSEA LSESESLSTS ESLSESESLS ASESISESEY
     LSESESLSAS ESLSESESIS ASESLSESES LSESESLSES ESLSTSESLS ASESLSESES
     LSESESLSES ESLSESESLS ESESLSDSES LSESESLSES ESLSESESLS DSESLSESES
     LSESESLSES ESLSESESLS ESELLSESES LSESESISES ESISASESLS ESESLSESES
     LSESESLSES ESLSESESLS ESESLSESES LSASESISAS ESLSESESLS ESESLSESES
     LSESESLSTS ESLSESESLS TSESISESES LSESESLSES ESLSESESLS KSESLSESES
     LSESESLSTS ESLSESESLS ASESISESES LSESESLSES ESLSTSESLR QSESLSQSLS
     LSASESLSAS ESISESESLS ESESLSASES LSESESLSAS ESLSESESLS ESESLSGSES
     LSASESLSES ESLSESESLS ESESLSESES LSGSESLSAS ESISESESIS ASESLSESES
     ISASESISES ESLSTSESLS TSESLSESES LSASESLSES ESLSESESLS ESESLSESES
     LSASESLSAS ESLSESESLS ESESLSESES LSESESLSES ESISESESLS ESESLSESES
     LSESESISDS ESISESESLS ESESLSESES ISESESLSES ESLSESESLS ESESISDSES
     LSESESLSES ESLSESESLS ESESLSESES LSASESLSAS ESLSASESLS ESESLSDSES
     LSESESLSES ESLSASESLS ASESLSESES LSESESISDS ESLSTSESLS ESESLSESES
     LSGSESLSAS ESLSASESLS ASESLSASES ISESESLSES ESLSESESLS DSESLSESES
     LSTSESLSAS ESLSESESLN TSESMSESES LGASESISEY ESLNRHLNND GNYEKEKDKL
     PDTGNEDKHN GLIPLLTALG GIILLRRRRN NEIQDK