UB12L_ARATH
ID UB12L_ARATH Reviewed; 185 AA.
AC Q9ZU75;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 136.
DE RecName: Full=Probable NEDD8-conjugating enzyme Ubc12-like;
DE EC=2.3.2.-;
DE AltName: Full=RUB1 carrier protein 2;
DE AltName: Full=RUB1-conjugating enzyme 2;
GN Name=RCE2; OrderedLocusNames=At2g18600; ORFNames=F24H14.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8/RUB1 from the ECR1-
CC AXR1 E1 complex and catalyzes its covalent attachment to other
CC proteins. {ECO:0000305}.
CC -!- PATHWAY: Protein modification; protein neddylation.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBC12
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; AC006135; AAD12207.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06784.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62258.1; -; Genomic_DNA.
DR EMBL; BT003913; AAO41960.1; -; mRNA.
DR EMBL; BT005005; AAO50538.1; -; mRNA.
DR PIR; C84566; C84566.
DR RefSeq; NP_001324430.1; NM_001335600.1.
DR RefSeq; NP_565440.1; NM_127416.4.
DR AlphaFoldDB; Q9ZU75; -.
DR SMR; Q9ZU75; -.
DR STRING; 3702.AT2G18600.1; -.
DR PaxDb; Q9ZU75; -.
DR PRIDE; Q9ZU75; -.
DR ProteomicsDB; 243236; -.
DR EnsemblPlants; AT2G18600.1; AT2G18600.1; AT2G18600.
DR EnsemblPlants; AT2G18600.2; AT2G18600.2; AT2G18600.
DR GeneID; 816375; -.
DR Gramene; AT2G18600.1; AT2G18600.1; AT2G18600.
DR Gramene; AT2G18600.2; AT2G18600.2; AT2G18600.
DR KEGG; ath:AT2G18600; -.
DR Araport; AT2G18600; -.
DR TAIR; locus:2046303; AT2G18600.
DR eggNOG; KOG0420; Eukaryota.
DR HOGENOM; CLU_030988_6_0_1; -.
DR InParanoid; Q9ZU75; -.
DR OMA; MPFAIMI; -.
DR OrthoDB; 1302735at2759; -.
DR PhylomeDB; Q9ZU75; -.
DR UniPathway; UPA00885; -.
DR PRO; PR:Q9ZU75; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZU75; baseline and differential.
DR Genevisible; Q9ZU75; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0019788; F:NEDD8 transferase activity; IBA:GO_Central.
DR GO; GO:0045116; P:protein neddylation; IBA:GO_Central.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..185
FT /note="Probable NEDD8-conjugating enzyme Ubc12-like"
FT /id="PRO_0000082495"
FT DOMAIN 31..176
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT REGION 8..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 114
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 185 AA; 21133 MW; 525FCEF868ACC730 CRC64;
MIGLFKVKEK QREESQSNNG RGASTVKKQS AGELRLHKDI SELNLPKSCK ISFPNGKNDL
MNFEVTIKPD EGYYLSGNFV FSFQVSNMYP HEAPKVKCKT KVYHPNIDLE GNVCLNILRE
DWKPVLNINT VIYGLFHLFT EPNYEDPLNH EAAAVLRDNP KTFEYNVRRA MMGGQVGQTS
FPRCM
