UB2D1_BOVIN
ID UB2D1_BOVIN Reviewed; 147 AA.
AC Q2TA10;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 D1;
DE EC=2.3.2.23;
DE AltName: Full=(E3-independent) E2 ubiquitin-conjugating enzyme D1;
DE EC=2.3.2.24;
DE AltName: Full=E2 ubiquitin-conjugating enzyme D1;
DE AltName: Full=Ubiquitin carrier protein D1;
DE AltName: Full=Ubiquitin-protein ligase D1;
GN Name=UBE2D1;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its
CC covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-
CC linked polyubiquitination. Mediates the selective degradation of short-
CC lived and abnormal proteins. Functions in the E6/E6-AP-induced
CC ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-
CC ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-
CC associated HSP90AB1 in vitro. Lacks inherent specificity for any
CC particular lysine residue of ubiquitin. Essential for viral activation
CC of IRF3. Mediates polyubiquitination of CYP3A4.
CC {ECO:0000250|UniProtKB:P51668}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000250|UniProtKB:P51668, ECO:0000255|PROSITE-
CC ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-
CC cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.24; Evidence={ECO:0000250|UniProtKB:P51668};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SUBUNIT: Component of a E3 ubiquitin ligase complex containing UBE2D1,
CC SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts with RNF11.
CC {ECO:0000250|UniProtKB:P51668}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P51668}.
CC -!- PTM: Autoubiquitinated. {ECO:0000250|UniProtKB:P51668}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; BC111175; AAI11176.1; -; mRNA.
DR RefSeq; NP_001033256.1; NM_001038167.1.
DR AlphaFoldDB; Q2TA10; -.
DR BMRB; Q2TA10; -.
DR SMR; Q2TA10; -.
DR STRING; 9913.ENSBTAP00000051358; -.
DR PaxDb; Q2TA10; -.
DR PRIDE; Q2TA10; -.
DR Ensembl; ENSBTAT00000055368; ENSBTAP00000051358; ENSBTAG00000020796.
DR GeneID; 535287; -.
DR KEGG; bta:535287; -.
DR CTD; 7321; -.
DR VEuPathDB; HostDB:ENSBTAG00000020796; -.
DR VGNC; VGNC:36579; UBE2D1.
DR eggNOG; KOG0417; Eukaryota.
DR GeneTree; ENSGT00940000155109; -.
DR HOGENOM; CLU_030988_13_3_1; -.
DR InParanoid; Q2TA10; -.
DR OMA; FCELNRE; -.
DR OrthoDB; 1337945at2759; -.
DR TreeFam; TF101108; -.
DR Reactome; R-BTA-1234176; Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
DR Reactome; R-BTA-5689896; Ovarian tumor domain proteases.
DR Reactome; R-BTA-8866652; Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
DR Reactome; R-BTA-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR Reactome; R-BTA-9705462; Inactivation of CSF3 (G-CSF) signaling.
DR Reactome; R-BTA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000009136; Chromosome 26.
DR Bgee; ENSBTAG00000020796; Expressed in supraspinatus muscle and 103 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000151; C:ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:Ensembl.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR GO; GO:0031398; P:positive regulation of protein ubiquitination; IEA:Ensembl.
DR GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Nucleotide-binding; Reference proteome;
KW Transferase; Ubl conjugation; Ubl conjugation pathway.
FT CHAIN 1..147
FT /note="Ubiquitin-conjugating enzyme E2 D1"
FT /id="PRO_0000245033"
FT DOMAIN 1..147
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 85
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 147 AA; 16602 MW; 2E96FD0179EE119D CRC64;
MALKRIQKEL SDLQRDPPAH CSAGPVGDDL FHWQATIMGP PDSAYQGGVF FLTVHFPTDY
PFKPPKIAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV
PDIAQIYKSD KEKYNRHARE WTQKYAM
