UB2D4_XENLA
ID UB2D4_XENLA Reviewed; 147 AA.
AC A5PKP9;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 D4 {ECO:0000250|UniProtKB:Q9Y2X8};
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme D4 {ECO:0000250|UniProtKB:Q9Y2X8};
DE AltName: Full=Ubiquitin carrier protein D4 {ECO:0000250|UniProtKB:Q9Y2X8};
DE AltName: Full=Ubiquitin-protein ligase D4 {ECO:0000250|UniProtKB:Q9Y2X8};
GN Name=ube2d4; Synonyms=ube2d3.2 {ECO:0000303|PubMed:18021256};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MAP3K10, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF CYS-85.
RX PubMed=18021256; DOI=10.1111/j.1432-0436.2007.00239.x;
RA Jean S., Moss T.;
RT "A ubiquitin-conjugating enzyme, ube2d3.2, regulates xMLK2 and pronephros
RT formation in Xenopus.";
RL Differentiation 76:431-441(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Neurula {ECO:0000312|EMBL:AAI42570.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC proteins. Regulates pronephros development, possibly by promoting
CC ubiquitination and thus inactivation or degradation of map3k10/mlk2.
CC {ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000269|PubMed:18021256}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000250|UniProtKB:Q9Y2X8, ECO:0000255|PROSITE-
CC ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:Q9Y2X8, ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SUBUNIT: Interacts with map3k10/mlk2. {ECO:0000269|PubMed:18021256}.
CC -!- TISSUE SPECIFICITY: At embryonic stages 28 to 35, expressed in the
CC somites, eye primordia, otic vesicle and branchial arches. By stage 35,
CC also weakly expressed in the pronephros. {ECO:0000269|PubMed:18021256}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally. Present at near constant
CC levels throughout early development. {ECO:0000269|PubMed:18021256}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; EU124673; ABV25624.1; -; mRNA.
DR EMBL; BC142569; AAI42570.1; -; mRNA.
DR RefSeq; NP_001093355.1; NM_001099885.1.
DR RefSeq; XP_018110595.1; XM_018255106.1.
DR AlphaFoldDB; A5PKP9; -.
DR SMR; A5PKP9; -.
DR GeneID; 100101299; -.
DR GeneID; 108712728; -.
DR KEGG; xla:100101299; -.
DR KEGG; xla:108712728; -.
DR CTD; 100101299; -.
DR CTD; 108712728; -.
DR Xenbase; XB-GENE-962538; ube2d4.L.
DR Xenbase; XB-GENE-17330936; ube2d4.S.
DR OrthoDB; 1337945at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000186698; Chromosome 3L.
DR Proteomes; UP000186698; Chromosome 3S.
DR Bgee; 100101299; Expressed in muscle tissue and 19 other tissues.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0031435; F:mitogen-activated protein kinase kinase kinase binding; IPI:UniProtKB.
DR GO; GO:0043130; F:ubiquitin binding; IDA:UniProtKB.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:UniProtKB.
DR GO; GO:0039020; P:pronephric nephron tubule development; IMP:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Developmental protein; Nucleotide-binding; Reference proteome;
KW Transferase; Ubl conjugation pathway.
FT CHAIN 1..147
FT /note="Ubiquitin-conjugating enzyme E2 D4"
FT /id="PRO_0000397918"
FT DOMAIN 1..147
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 85
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT MUTAGEN 85
FT /note="C->A: Catalytically inactive. No effect on
FT pronephros development."
FT /evidence="ECO:0000269|PubMed:18021256"
SQ SEQUENCE 147 AA; 16677 MW; 3832EF718DF47EC2 CRC64;
MALKRIQKEL MDLQRDPPAQ CSAGPVGEDL FHWQATIMGP NDSPFQGGVF FLTIHFPTDY
PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV
PEIAHTYKAD REKYNRLARE WTQKYAM
