UB2G2_BOVIN
ID UB2G2_BOVIN Reviewed; 165 AA.
AC Q17QG5;
DT 14-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 G2;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme G2;
DE AltName: Full=Ubiquitin carrier protein G2;
DE AltName: Full=Ubiquitin-protein ligase G2;
GN Name=UBE2G2 {ECO:0000250|UniProtKB:P60604};
GN Synonyms=UBC7 {ECO:0000250|UniProtKB:P60604};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal pons;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its
CC covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-
CC linked polyubiquitination. Involved in endoplasmic reticulum-associated
CC degradation (ERAD). Required for sterol-induced ubiquitination of 3-
CC hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent
CC proteasomal degradation. {ECO:0000250|UniProtKB:P60604}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000250|UniProtKB:P60604, ECO:0000255|PROSITE-
CC ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SUBUNIT: Interacts with AUP1 (via C-terminus); the interaction recruits
CC UBE2G2 to lipid droplets. Interacts with ubiquitin ligases AMFR/gp78
CC and RNF139/TRC8; recruitment to lipid droplets by AUP1 facilitates
CC interaction of UBE2G2 with AMFR and RNF139, leading to sterol-induced
CC ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and
CC its subsequent proteasomal degradation. {ECO:0000250|UniProtKB:P60604}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:P60605}. Lipid droplet
CC {ECO:0000250|UniProtKB:P60604}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; BT026143; ABG66982.1; -; mRNA.
DR EMBL; BC118376; AAI18377.1; -; mRNA.
DR RefSeq; NP_001069796.1; NM_001076328.1.
DR AlphaFoldDB; Q17QG5; -.
DR BMRB; Q17QG5; -.
DR SMR; Q17QG5; -.
DR STRING; 9913.ENSBTAP00000055782; -.
DR PaxDb; Q17QG5; -.
DR Ensembl; ENSBTAT00000063929; ENSBTAP00000055782; ENSBTAG00000048090.
DR GeneID; 614471; -.
DR KEGG; bta:614471; -.
DR CTD; 7327; -.
DR VEuPathDB; HostDB:ENSBTAG00000048090; -.
DR VGNC; VGNC:36582; UBE2G2.
DR eggNOG; KOG0426; Eukaryota.
DR GeneTree; ENSGT00940000158193; -.
DR HOGENOM; CLU_030988_10_1_1; -.
DR InParanoid; Q17QG5; -.
DR OMA; KMWRENR; -.
DR OrthoDB; 1317014at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000009136; Chromosome 1.
DR Bgee; ENSBTAG00000048090; Expressed in vas deferens and 107 other tissues.
DR ExpressionAtlas; Q17QG5; baseline and differential.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005811; C:lipid droplet; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR GO; GO:0035458; P:cellular response to interferon-beta; IEA:Ensembl.
DR GO; GO:1904153; P:negative regulation of retrograde protein transport, ER to cytosol; IEA:Ensembl.
DR GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; ISS:UniProtKB.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW Acetylation; ATP-binding; Endoplasmic reticulum; Lipid droplet;
KW Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P60604"
FT CHAIN 2..165
FT /note="Ubiquitin-conjugating enzyme E2 G2"
FT /id="PRO_0000259961"
FT DOMAIN 4..164
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 89
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P60604"
SQ SEQUENCE 165 AA; 18552 MW; 74DEC72A049575E3 CRC64;
MAGTALKRLM AEYKQLTLNP PEGIVAGPMN EENFFEWEAL IMGPEDTCFE FGVFPAILSF
PLDYPLSPPK MRFTCEMFHP NIYPDGRVCI SILHAPGDDP MGYESSAERW SPVQSVEKIL
LSVVSMLAEP NDESGANVDA SKMWRDDREQ FYKVAKQIVQ KSLGL
