ID   UB2J2_BOVIN             Reviewed;         259 AA.
AC   Q2TA03;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 J2;
DE            EC=2.3.2.23;
DE   AltName: Full=E2 ubiquitin-conjugating enzyme J2;
GN   Name=UBE2J2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. Seems to function in the selective degradation of misfolded
CC       membrane proteins from the endoplasmic reticulum (ERAD).
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC         [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC         activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00388};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q9Y385}; Single-pass type IV membrane protein
CC       {ECO:0000250|UniProtKB:Q9Y385}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; BC111185; AAI11186.1; -; mRNA.
DR   RefSeq; NP_001033132.1; NM_001038043.1.
DR   AlphaFoldDB; Q2TA03; -.
DR   SMR; Q2TA03; -.
DR   STRING; 9913.ENSBTAP00000021109; -.
DR   PaxDb; Q2TA03; -.
DR   PRIDE; Q2TA03; -.
DR   Ensembl; ENSBTAT00000021109; ENSBTAP00000021109; ENSBTAG00000015882.
DR   Ensembl; ENSBTAT00000078635; ENSBTAP00000069660; ENSBTAG00000015882.
DR   GeneID; 506103; -.
DR   KEGG; bta:506103; -.
DR   CTD; 118424; -.
DR   VEuPathDB; HostDB:ENSBTAG00000015882; -.
DR   VGNC; VGNC:36586; UBE2J2.
DR   eggNOG; KOG0894; Eukaryota.
DR   GeneTree; ENSGT00940000156173; -.
DR   HOGENOM; CLU_041481_1_2_1; -.
DR   InParanoid; Q2TA03; -.
DR   OMA; DKMFCEL; -.
DR   OrthoDB; 1230974at2759; -.
DR   TreeFam; TF101123; -.
DR   Reactome; R-BTA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000009136; Chromosome 16.
DR   Bgee; ENSBTAG00000015882; Expressed in spermatid and 102 other tissues.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000151; C:ubiquitin ligase complex; IEA:Ensembl.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR   GO; GO:0031625; F:ubiquitin protein ligase binding; IEA:Ensembl.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   GO; GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW.
DR   GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IBA:GO_Central.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Endoplasmic reticulum; Membrane; Nucleotide-binding;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix;
KW   Ubl conjugation pathway; Unfolded protein response.
FT   CHAIN           1..259
FT                   /note="Ubiquitin-conjugating enzyme E2 J2"
FT                   /id="PRO_0000245041"
FT   TOPO_DOM        1..226
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        227..247
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        248..259
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          12..162
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   REGION          174..200
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        94
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
SQ   SEQUENCE   259 AA;  28848 MW;  AFF8D77BD15BF8A4 CRC64;
     MSSNSVKRAP TTATQRLKQD YLRIKKDPVP YICAEPLPSN ILEWHYVVRG PEMTPYEGGY
     YHGKLIFPRE FPFKPPSIYM ITPNGRFKCN TRLCLSITDF HPDTWNPAWS VSTILTGLLS
     FMVEKGPTLG SIETSDFTKR QLAAQSLVFN LKDKVFCELF PEVVEEIKQK QKAQDELSSR
     PQALPLPDVV PDGETHHGQH GLPLLNGHAP GAGPHLAGLQ QANRHHGLLG GALANLFVIV
     GFAAFAYTVK YVLRSIAQE