ID   UB2L6_RAT               Reviewed;         153 AA.
AC   Q4V8J2;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Ubiquitin/ISG15-conjugating enzyme E2 L6;
DE            EC=2.3.2.23;
DE   AltName: Full=E2 ubiquitin-conjugating enzyme L6;
DE   AltName: Full=Ubiquitin carrier protein L6;
DE   AltName: Full=Ubiquitin-protein ligase L6;
GN   Name=Ube2l6;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
CC       Promotes ubiquitination and subsequent proteasomal degradation of FLT3.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC         [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC         activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC         ProRule:PRU10133};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- SUBUNIT: Interacts with RNF19A, RNF19B and RNF144B. Interacts with FLT3
CC       (tyrosine phosphorylated). {ECO:0000250|UniProtKB:O14933}.
CC   -!- PTM: ISGylated. {ECO:0000250|UniProtKB:O14933}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; BC097364; AAH97364.1; -; mRNA.
DR   RefSeq; NP_001019926.1; NM_001024755.1.
DR   AlphaFoldDB; Q4V8J2; -.
DR   SMR; Q4V8J2; -.
DR   STRING; 10116.ENSRNOP00000039001; -.
DR   jPOST; Q4V8J2; -.
DR   PaxDb; Q4V8J2; -.
DR   PRIDE; Q4V8J2; -.
DR   Ensembl; ENSRNOT00000109055; ENSRNOP00000084777; ENSRNOG00000030467.
DR   GeneID; 295704; -.
DR   KEGG; rno:295704; -.
DR   UCSC; RGD:1307960; rat.
DR   CTD; 9246; -.
DR   RGD; 1307960; Ube2l6.
DR   eggNOG; KOG0422; Eukaryota.
DR   GeneTree; ENSGT00940000161981; -.
DR   HOGENOM; CLU_030988_13_3_1; -.
DR   InParanoid; Q4V8J2; -.
DR   OMA; RPNLEEP; -.
DR   OrthoDB; 1420213at2759; -.
DR   PhylomeDB; Q4V8J2; -.
DR   TreeFam; TF313043; -.
DR   Reactome; R-RNO-1169408; ISG15 antiviral mechanism.
DR   Reactome; R-RNO-5656169; Termination of translesion DNA synthesis.
DR   Reactome; R-RNO-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q4V8J2; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   Bgee; ENSRNOG00000030467; Expressed in duodenum and 19 other tissues.
DR   Genevisible; Q4V8J2; RN.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000151; C:ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042296; F:ISG15 transferase activity; ISO:RGD.
DR   GO; GO:0043130; F:ubiquitin binding; ISO:RGD.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR   GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR   GO; GO:0019787; F:ubiquitin-like protein transferase activity; ISO:RGD.
DR   GO; GO:0032020; P:ISG15-protein conjugation; ISO:RGD.
DR   GO; GO:0019941; P:modification-dependent protein catabolic process; ISO:RGD.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW   Ubl conjugation; Ubl conjugation pathway.
FT   CHAIN           1..153
FT                   /note="Ubiquitin/ISG15-conjugating enzyme E2 L6"
FT                   /id="PRO_0000375221"
FT   DOMAIN          2..149
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   ACT_SITE        86
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   153 AA;  17807 MW;  47E1AC421ADC756E CRC64;
     MTASKRVAKE LDDLSKELPP YLRHLSSDDA NVLVWHMLLL PDQLPYRLKA FGLRIDFPRE
     YPLKPPTLRF TTKIYHPNIS EDGLVCLPLI STENWKPYTK AYQVLEALNI LVSRPNLEEP
     VRLELADLLT QDPEMFRKKA EEFTLQYGVD RPS