UB2SC_XENLA
ID UB2SC_XENLA Reviewed; 211 AA.
AC Q76EZ2; Q5U4M8;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 S-C;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme S-C;
DE AltName: Full=Ubiquitin carrier protein S-C;
DE AltName: Full=Ubiquitin-protein ligase S-C;
GN Name=ube2s-c; Synonyms=ube2s.2;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Oocyte;
RA Karasawa A., Matushita H., Yagura T.;
RT "Molecular cloning of cDNAs encoding Xenopus ubiquitin-conjugating
RT enzyme(E2) and their expression in various Xenopus tissues.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Gastrula;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC proteins. Acts as an essential factor of the anaphase promoting
CC complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that
CC controls progression through mitosis. Acts by specifically elongating
CC 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme
CC ube2c/ubch10 on APC/C substrates, enhancing the degradation of APC/C
CC substrates by the proteasome and promoting mitotic exit.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q76EZ2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q76EZ2-2; Sequence=VSP_038532;
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; AB105547; BAD06216.1; -; mRNA.
DR EMBL; BC169403; AAI69403.1; -; mRNA.
DR EMBL; BC169405; AAI69405.1; -; mRNA.
DR EMBL; BC085030; AAH85030.1; -; mRNA.
DR RefSeq; NP_001084432.1; NM_001090963.1. [Q76EZ2-1]
DR RefSeq; XP_018079916.1; XM_018224427.1. [Q76EZ2-1]
DR AlphaFoldDB; Q76EZ2; -.
DR SMR; Q76EZ2; -.
DR GeneID; 403383; -.
DR KEGG; xla:403383; -.
DR CTD; 403383; -.
DR Xenbase; XB-GENE-6255548; ube2s.L.
DR OMA; WTCLLTH; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000186698; Chromosome 7L.
DR Bgee; 403383; Expressed in egg cell and 19 other tissues.
DR GO; GO:0005680; C:anaphase-promoting complex; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; ISS:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0010458; P:exit from mitosis; ISS:UniProtKB.
DR GO; GO:0010994; P:free ubiquitin chain polymerization; ISS:UniProtKB.
DR GO; GO:1904668; P:positive regulation of ubiquitin protein ligase activity; ISS:UniProtKB.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Cell cycle; Cell division;
KW Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..211
FT /note="Ubiquitin-conjugating enzyme E2 S-C"
FT /id="PRO_0000390433"
FT DOMAIN 11..157
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT REGION 158..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 164..178
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 95
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
FT VAR_SEQ 1..61
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_038532"
SQ SEQUENCE 211 AA; 23338 MW; C0EC23D41938D5E9 CRC64;
MNSNVENLPP HIIRRVYKEV STLTSDPPEG IKIIPNEEDI TDVQVHIEGP EGTPYAAGIF
RMKLILGKDF PAAPPKGYFL TKIFHPNVSN NGEICVNVLK KDWKAELGIR HVLLTIKCLL
IHPNPESALN EEAGRLLLEN YEEYASRAKL MTEIHAQGST LRGKDPTDPC SSASATVVSG
DGPMAKKHAG DRDKKLAAKK KTDKKRALRR L
