C78A5_ARATH
ID C78A5_ARATH Reviewed; 517 AA.
AC Q9LMX7;
DT 24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Cytochrome P450 78A5;
DE EC=1.14.-.-;
DE AltName: Full=Protein KLUH;
GN Name=CYP78A5; Synonyms=KLU; OrderedLocusNames=At1g13710;
GN ORFNames=F21F23.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10476073; DOI=10.1046/j.1365-313x.1999.00523.x;
RA Zondlo S.C., Irish V.F.;
RT "CYP78A5 encodes a cytochrome P450 that marks the shoot apical meristem
RT boundary in Arabidopsis.";
RL Plant J. 19:259-268(1999).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=18061566; DOI=10.1016/j.devcel.2007.10.001;
RA Anastasiou E., Kenz S., Gerstung M., MacLean D., Timmer J., Fleck C.,
RA Lenhard M.;
RT "Control of plant organ size by KLUH/CYP78A5-dependent intercellular
RT signaling.";
RL Dev. Cell 13:843-856(2007).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=18492871; DOI=10.1105/tpc.108.058180;
RA Wang J.W., Schwab R., Czech B., Mica E., Weigel D.;
RT "Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron
RT length and organ size in Arabidopsis thaliana.";
RL Plant Cell 20:1231-1243(2008).
RN [7]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=19892740; DOI=10.1073/pnas.0907024106;
RA Adamski N.M., Anastasiou E., Eriksson S., O'Neill C.M., Lenhard M.;
RT "Local maternal control of seed size by KLUH/CYP78A5-dependent growth
RT signaling.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:20115-20120(2009).
RN [8]
RP FUNCTION.
RX PubMed=20188559; DOI=10.1016/j.cub.2010.01.039;
RA Eriksson S., Stransfeld L., Adamski N.M., Breuninger H., Lenhard M.;
RT "KLUH/CYP78A5-dependent growth signaling coordinates floral organ growth in
RT Arabidopsis.";
RL Curr. Biol. 20:527-532(2010).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=20395288; DOI=10.1093/pcp/pcq051;
RA Kazama T., Ichihashi Y., Murata S., Tsukaya H.;
RT "The mechanism of cell cycle arrest front progression explained by a
RT KLUH/CYP78A5-dependent mobile growth factor in developing leaves of
RT Arabidopsis thaliana.";
RL Plant Cell Physiol. 51:1046-1054(2010).
CC -!- FUNCTION: Plays a role in regulating directional growth at the
CC meristem/organ boundary. Is required for the promotion of leaf and
CC floral organ growth and for the prolongation of the plastochron.
CC Promotes organ growth in a non-cell-autonomous manner and may generate
CC a mobile growth signal distinct from the classical phytohormones that
CC prevents premature arrest of proliferation, until the correct
CC primordium size has been reached. Functions probably in association
CC with CYP78A7 in regulating relative growth of the shoot apical meristem
CC and plant organs. Is required locally in developing ovules to
CC stimulates cell proliferation and promote seed growth.
CC {ECO:0000269|PubMed:10476073, ECO:0000269|PubMed:18061566,
CC ECO:0000269|PubMed:18492871, ECO:0000269|PubMed:19892740,
CC ECO:0000269|PubMed:20188559}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the periphery of the shoot apical
CC meristem and inflorescence meristem, on the adaxial sides of developing
CC floral organs and in developing ovules in the region where the
CC integuments emerge. {ECO:0000269|PubMed:10476073,
CC ECO:0000269|PubMed:18061566, ECO:0000269|PubMed:20395288}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the inner integument throughout ovule
CC development. {ECO:0000269|PubMed:19892740}.
CC -!- DISRUPTION PHENOTYPE: Reduced size of leaves, sepals, petals and seeds.
CC Increased leaf number. {ECO:0000269|PubMed:18061566,
CC ECO:0000269|PubMed:18492871}.
CC -!- MISCELLANEOUS: Plants over-expressing CYP78A5 have twisted and kinked
CC stems, defects in floral organogenesis and reduced fertility.
CC {ECO:0000305|PubMed:10476073}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AC027656; AAF81298.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29060.1; -; Genomic_DNA.
DR EMBL; BT022025; AAY25437.1; -; mRNA.
DR EMBL; BT029218; ABJ17153.1; -; mRNA.
DR PIR; E86270; E86270.
DR RefSeq; NP_172827.1; NM_101240.4.
DR AlphaFoldDB; Q9LMX7; -.
DR SMR; Q9LMX7; -.
DR STRING; 3702.AT1G13710.1; -.
DR PaxDb; Q9LMX7; -.
DR PRIDE; Q9LMX7; -.
DR EnsemblPlants; AT1G13710.1; AT1G13710.1; AT1G13710.
DR GeneID; 837932; -.
DR Gramene; AT1G13710.1; AT1G13710.1; AT1G13710.
DR KEGG; ath:AT1G13710; -.
DR Araport; AT1G13710; -.
DR TAIR; locus:2023848; AT1G13710.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_0_1; -.
DR InParanoid; Q9LMX7; -.
DR OMA; ATVHLWI; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; Q9LMX7; -.
DR PRO; PR:Q9LMX7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LMX7; baseline and differential.
DR Genevisible; Q9LMX7; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0048437; P:floral organ development; IMP:TAIR.
DR GO; GO:0035265; P:organ growth; IMP:TAIR.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:TAIR.
DR GO; GO:0046622; P:positive regulation of organ growth; IMP:TAIR.
DR GO; GO:0040009; P:regulation of growth rate; IMP:TAIR.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Growth regulation; Heme; Iron; Membrane;
KW Metal-binding; Monooxygenase; Oxidoreductase; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..517
FT /note="Cytochrome P450 78A5"
FT /id="PRO_0000422986"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 459
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 517 AA; 57644 MW; 1137666E3A7E129E CRC64;
MSPEAYVLFF NSFNLVTFEA FASVSLIIAT VAFLLSPGGL AWAWTGSSKS RVSIPGPSGS
LSVFSGSNPH RVLAALAKRF KASPLMAFSV GFSRFVISSE PETAKEILSS SAFADRPVKE
SAYELLFHRA MGFAPYGEYW RNLRRISSTH LFSPRRIASF EGVRVGIGMK MVKKIKSLVT
SDACGEVEVK KIVHFGSLNN VMTTVFGESY DFDEVNGKGC FLERLVSEGY ELLGIFNWSD
HFWFLRWFDF QGVRKRCRAL VSEVNTFVGG IIEKHKMKKG NNLNGEENDF VDVLLGLQKD
EKLSDSDMIA VLWEMIFRGT DTVAILVEWV LARMVLHQDI QDKLYREIAS ATSNNIRSLS
DSDIPKLPYL QAIVKETLRL HPPGPLLSWA RLAIHDVHVG PNLVPAGTIA MVNMWSITHN
AKIWTDPEAF MPERFISEDV SIMGSDLRLA PFGSGRRVCP GKAMGLATVH LWIGQLIQNF
EWVKGSCDVE LAEVLKLSME MKNPLKCKAV PRNVGFA
