C78A6_ARATH
ID C78A6_ARATH Reviewed; 530 AA.
AC Q9ZNR0;
DT 24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Cytochrome P450 78A6;
DE EC=1.14.-.-;
DE AltName: Full=Protein ENHANCER OF DA1-1;
GN Name=CYP78A6; Synonyms=EOD3; OrderedLocusNames=At2g46660; ORFNames=T3A4.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=22251317; DOI=10.1111/j.1365-313x.2012.04907.x;
RA Fang W., Wang Z., Cui R., Li J., Li Y.;
RT "Maternal control of seed size by EOD3/CYP78A6 in Arabidopsis thaliana.";
RL Plant J. 70:929-939(2012).
CC -!- FUNCTION: Plays role in seed and fruit development. Functions probably
CC in association with CYP78A9 in the regulation of seed growth. Acts
CC maternally to promote seed growth. {ECO:0000269|PubMed:22251317}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, sepals, petals, stamens,
CC carpels and developing ovules. {ECO:0000269|PubMed:22251317}.
CC -!- DISRUPTION PHENOTYPE: Reduced seed size. {ECO:0000269|PubMed:22251317}.
CC -!- MISCELLANEOUS: The gain of function mutants eod3-1D (T-DNA tagging)
CC show increased size of leaves, flowers and seeds, but defects in
CC reproductive development. {ECO:0000305|PubMed:22251317}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AC005819; AAC69923.1; -; Genomic_DNA.
DR EMBL; AC006418; AAM15240.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10736.1; -; Genomic_DNA.
DR EMBL; AK226763; BAE98862.1; -; mRNA.
DR PIR; F84905; F84905.
DR RefSeq; NP_182189.1; NM_130231.4.
DR AlphaFoldDB; Q9ZNR0; -.
DR SMR; Q9ZNR0; -.
DR STRING; 3702.AT2G46660.1; -.
DR PaxDb; Q9ZNR0; -.
DR PRIDE; Q9ZNR0; -.
DR EnsemblPlants; AT2G46660.1; AT2G46660.1; AT2G46660.
DR GeneID; 819278; -.
DR Gramene; AT2G46660.1; AT2G46660.1; AT2G46660.
DR KEGG; ath:AT2G46660; -.
DR Araport; AT2G46660; -.
DR TAIR; locus:2039954; AT2G46660.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_0_1; -.
DR InParanoid; Q9ZNR0; -.
DR OMA; RYWWNRR; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; Q9ZNR0; -.
DR BioCyc; ARA:AT2G46660-MON; -.
DR PRO; PR:Q9ZNR0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZNR0; baseline and differential.
DR Genevisible; Q9ZNR0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0048316; P:seed development; IMP:TAIR.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Growth regulation; Heme; Iron; Membrane;
KW Metal-binding; Monooxygenase; Oxidoreductase; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..530
FT /note="Cytochrome P450 78A6"
FT /id="PRO_0000422988"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 474
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 530 AA; 59565 MW; 87CDA9DFC395D62F CRC64;
MATKLESSLI FALLSKCSVL SQTNLAFSLL AVTIIWLAIS LFLWTYPGGP AWGKYLFGRL
ISGSYKTGNV IPGPKGFPLV GSMSLMSSTL AHRRIADAAE KFGAKRLMAF SLGETRVIVT
CNPDVAKEIL NSPVFADRPV KESAYSLMFN RAIGFAPHGV YWRTLRRIAS NHLFSTKQIR
RAETQRRVIS SQMVEFLEKQ SSNEPCFVRE LLKTASLNNM MCSVFGQEYE LEKNHVELRE
MVEEGYDLLG TLNWTDHLPW LSEFDPQRLR SRCSTLVPKV NRFVSRIISE HRNQTGDLPR
DFVDVLLSLH GSDKLSDPDI IAVLWEMIFR GTDTVAVLIE WILARMVLHP DMQSTVQNEL
DQVVGKSRAL DESDLASLPY LTAVVKEVLR LHPPGPLLSW ARLAITDTIV DGRLVPAGTT
AMVNMWAVSH DPHVWVDPLE FKPERFVAKE GEVEFSVLGS DLRLAPFGSG RRICPGKNLG
FTTVMFWTAM MLHEFEWGPS DGNGVDLSEK LRLSCEMANP LPAKLRRRRS
