ID   UBA5_BOMMO              Reviewed;         393 AA.
AC   B9VJ80;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Ubiquitin-like modifier-activating enzyme 5;
DE            Short=Ubiquitin-activating enzyme 5;
OS   Bombyx mori (Silk moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Bombycidae; Bombycinae; Bombyx.
OX   NCBI_TaxID=7091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Wu P., Guo X.-J., Li M.-W.;
RL   Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E1-like enzyme which activates UFM1. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family. UBA5
CC       subfamily. {ECO:0000305}.
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DR   EMBL; FJ556993; ACL99855.1; -; mRNA.
DR   RefSeq; NP_001138805.1; NM_001145333.1.
DR   AlphaFoldDB; B9VJ80; -.
DR   SMR; B9VJ80; -.
DR   STRING; 7091.BGIBMGA013537-TA; -.
DR   PRIDE; B9VJ80; -.
DR   GeneID; 100270786; -.
DR   KEGG; bmor:100270786; -.
DR   eggNOG; KOG2336; Eukaryota.
DR   HOGENOM; CLU_013325_0_0_1; -.
DR   OrthoDB; 1092362at2759; -.
DR   Proteomes; UP000005204; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008641; F:ubiquitin-like modifier activating enzyme activity; IEA:InterPro.
DR   InterPro; IPR029752; D-isomer_DH_CS1.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   PANTHER; PTHR10953; PTHR10953; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Metal-binding; Nucleotide-binding; Reference proteome;
KW   Ubl conjugation pathway; Zinc.
FT   CHAIN           1..393
FT                   /note="Ubiquitin-like modifier-activating enzyme 5"
FT                   /id="PRO_0000391939"
FT   ACT_SITE        241
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   BINDING         75
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         96
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         119
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         142
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         175
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         217
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         220
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         294
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         299
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   393 AA;  42531 MW;  A8B2FE039515DEAB CRC64;
     MASVDELQKK IKELEAKLAA VEAKGGPMRQ KIEVMSSEVV DSNPYSRLMA LKRMGIVNNY
     EQIREKTVAV VGVGGVGSVT AEMLTRCGIG KLILFDYDKV ELANMNRLFF QPHQAGLSKV
     DAAAATLQNI NPDVTIDAYN YNITTVDNFQ KFCDTISKGS LTGGAVDLVL SCVDNFEARM
     AINTACNELD QKWFESGVSE NAVSGHIQFI SPGESACFAC APPLVVATKV DERTLKREGV
     CAASLPTTMG IVAGFLVQNS LKYLLEFGNV THYLGYSALT DFFPTMSLQP NPTCDDASCR
     ARQEQRRLQP RVELAAEVTE DCGPVHQDND WGISVLEENS PADEDCPGLK LVDGVQVAYS
     IPVDSSTPES STGGAVAASE LSLEDLMQQM KTM