ID   UBA5_CULQU              Reviewed;         397 AA.
AC   B0WQV1;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Ubiquitin-like modifier-activating enzyme 5;
DE            Short=Ubiquitin-activating enzyme 5;
GN   ORFNames=CPIJ009416;
OS   Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Culicini; Culex; Culex.
OX   NCBI_TaxID=7176;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JHB;
RG   The Broad Institute Genome Sequencing Platform;
RA   Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.D.,
RA   Hannick L.I., Megy K., O'Leary S.B., Pearson M., Haas B.J., Mauceli E.,
RA   Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P.,
RA   Antoine C.H., Arensburger P., Bidwell S.L., Crawford M., Camaro F.,
RA   Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D.,
RA   Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J.,
RA   Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E.,
RA   Fraser-Liggett C.M., Strausberg R., Galagan J., Birren B., Collins F.H.;
RT   "Annotation of Culex pipiens quinquefasciatus.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E1-like enzyme which activates UFM1. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family. UBA5
CC       subfamily. {ECO:0000305}.
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DR   EMBL; DS232046; EDS33031.1; -; Genomic_DNA.
DR   RefSeq; XP_001851085.1; XM_001851033.1.
DR   AlphaFoldDB; B0WQV1; -.
DR   SMR; B0WQV1; -.
DR   STRING; 7176.CPIJ009416-PA; -.
DR   KEGG; cqu:CpipJ_CPIJ009416; -.
DR   VEuPathDB; VectorBase:CPIJ009416; -.
DR   eggNOG; KOG2336; Eukaryota.
DR   HOGENOM; CLU_013325_0_1_1; -.
DR   InParanoid; B0WQV1; -.
DR   OMA; FTPDQAG; -.
DR   OrthoDB; 1092362at2759; -.
DR   PhylomeDB; B0WQV1; -.
DR   Proteomes; UP000002320; Partially assembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008641; F:ubiquitin-like modifier activating enzyme activity; IEA:InterPro.
DR   InterPro; IPR029752; D-isomer_DH_CS1.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   PANTHER; PTHR10953; PTHR10953; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Metal-binding; Nucleotide-binding; Reference proteome;
KW   Ubl conjugation pathway; Zinc.
FT   CHAIN           1..397
FT                   /note="Ubiquitin-like modifier-activating enzyme 5"
FT                   /id="PRO_0000391940"
FT   ACT_SITE        244
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   BINDING         77
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         98
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         121
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         144
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         178
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         220
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         223
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         297
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         302
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   397 AA;  43505 MW;  D68F55B599469634 CRC64;
     MTSVAELREQ VRSLQDELAQ LKGERGKTTT REKITKMSSE VVDSNPYSRL MALQRMGIVK
     EYEQIRQKSV AVVGVGGVGS VTADMLTRCG VGKLILFDYD KVELANMNRL FFTPDQAGLS
     KVEAAAKTLN YINPDVKIFT NNYNITTVES FEKFMNAIRT GGIDGSGAVD LVLSCVDNFE
     ARMAINAACN ELSLNWFESG VSENAVSGHI QFIQPGEKAC FACAPPLVVA ENIDEKTLKR
     EGVCAASLPT TMGIVAGMLV QNTLKYLLKF GTVSDYLGYN ALIDFFPKMG LKPNPTCDDR
     FCVLRQQEFA AKPKEETFEE VQQEEESPVH AENLYGIELV SETEVESAPT VPVATANTGL
     KLAFETPIQM EHSSAATDVI KNDDVSLDDL MAQMKAI