UBA5_ORYSJ
ID UBA5_ORYSJ Reviewed; 421 AA.
AC Q6K6K7; B9F062; Q6K6K5; Q6K6K6;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Ubiquitin-like modifier-activating enzyme 5;
DE Short=Ubiquitin-activating enzyme 5;
GN OrderedLocusNames=Os02g0506500, LOC_Os02g30310;
GN ORFNames=OsJ_06853, P0047E05.15;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: E1-like enzyme which activates UFM1. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6K6K7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6K6K7-2; Sequence=VSP_038771;
CC Name=3;
CC IsoId=Q6K6K7-3; Sequence=VSP_038772, VSP_038773;
CC -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family. UBA5
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EEE57054.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP005066; BAD23174.1; -; Genomic_DNA.
DR EMBL; AP005066; BAD23175.1; -; Genomic_DNA.
DR EMBL; AP005066; BAD23176.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08833.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS78830.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS78831.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS78832.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE57054.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK062572; BAG88369.1; -; mRNA.
DR EMBL; AK063549; BAG88762.1; -; mRNA.
DR EMBL; AK101016; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015625906.1; XM_015770420.1. [Q6K6K7-1]
DR RefSeq; XP_015625907.1; XM_015770421.1. [Q6K6K7-3]
DR AlphaFoldDB; Q6K6K7; -.
DR SMR; Q6K6K7; -.
DR STRING; 4530.OS02T0506500-04; -.
DR PaxDb; Q6K6K7; -.
DR PRIDE; Q6K6K7; -.
DR EnsemblPlants; Os02t0506500-01; Os02t0506500-01; Os02g0506500. [Q6K6K7-3]
DR EnsemblPlants; Os02t0506500-03; Os02t0506500-03; Os02g0506500. [Q6K6K7-2]
DR EnsemblPlants; Os02t0506500-04; Os02t0506500-04; Os02g0506500. [Q6K6K7-1]
DR GeneID; 4329438; -.
DR Gramene; Os02t0506500-01; Os02t0506500-01; Os02g0506500. [Q6K6K7-3]
DR Gramene; Os02t0506500-03; Os02t0506500-03; Os02g0506500. [Q6K6K7-2]
DR Gramene; Os02t0506500-04; Os02t0506500-04; Os02g0506500. [Q6K6K7-1]
DR KEGG; osa:4329438; -.
DR eggNOG; KOG2336; Eukaryota.
DR InParanoid; Q6K6K7; -.
DR OMA; FTPDQAG; -.
DR OrthoDB; 1548094at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR ExpressionAtlas; Q6K6K7; baseline and differential.
DR Genevisible; Q6K6K7; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0071566; F:UFM1 activating enzyme activity; IBA:GO_Central.
DR GO; GO:0032446; P:protein modification by small protein conjugation; IBA:GO_Central.
DR GO; GO:0071569; P:protein ufmylation; IBA:GO_Central.
DR InterPro; IPR029752; D-isomer_DH_CS1.
DR InterPro; IPR045886; ThiF/MoeB/HesA.
DR InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR InterPro; IPR035985; Ubiquitin-activating_enz.
DR PANTHER; PTHR10953; PTHR10953; 1.
DR Pfam; PF00899; ThiF; 1.
DR SUPFAM; SSF69572; SSF69572; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Metal-binding; Nucleotide-binding;
KW Reference proteome; Ubl conjugation pathway; Zinc.
FT CHAIN 1..421
FT /note="Ubiquitin-like modifier-activating enzyme 5"
FT /id="PRO_0000391954"
FT ACT_SITE 257
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000250"
FT BINDING 89
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 110
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 133
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 156
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 191
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 233
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 236
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 310
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 315
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT VAR_SEQ 28..47
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_038771"
FT VAR_SEQ 358..396
FT /note="SVVDDSDTVTPSILSTGADSLPEGLVRELPTADSYQEPV -> RFSCHTNGA
FT DKIVDLDRTLYPMLKPTSFLKHPACRIACV (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_038772"
FT VAR_SEQ 397..421
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_038773"
FT CONFLICT 38
FT /note="P -> S (in Ref. 5; AK101016)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 421 AA; 45624 MW; DBE8CCCFD26B7884 CRC64;
MDEEQLRALL RDLDALKQRP DPAAIDRMRE RVAGMVTPAA AARSKIKDMS SEVVDSNPYS
RLMALQRMGI VDNYERIRDY SIAIVGIGGV GSVAAEMLTR CGIGRLLLYD YDTVELANMN
RLFFRPDQVG MTKTDAAVQT LSGINPDVTL ESYSLNITTV KGFETFLGSL KARSSDGRNT
GVDLVLSCVD NYEARMVVNQ ACNELGQTWM ESGVSEDAVS GHIQLLVPGE TACFACAPPL
VVASGVDERT LKREGVCAAS LPTTMGVVAG LLVQNALKYL LKFGQVSPYL GYNSLKDYFP
TMEMKPNPQC SNPACVQRQK EYMQSKPARD AAAKAKMEAE ASAADECPVH LDNDWNISVV
DDSDTVTPSI LSTGADSLPE GLVRELPTAD SYQEPVAPVT SGAIDDDLEE LQRQLDALNS
S
