UBAC1_MOUSE
ID UBAC1_MOUSE Reviewed; 409 AA.
AC Q8VDI7; Q3TZH3;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Ubiquitin-associated domain-containing protein 1;
DE Short=UBA domain-containing protein 1;
DE AltName: Full=E3 ubiquitin-protein ligase subunit KPC2;
DE AltName: Full=Kip1 ubiquitination-promoting complex protein 2;
GN Name=Ubac1; Synonyms=Kpc2, Ubadc1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS GLY-252; ALA-269 AND
RP THR-274.
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Non-catalytic subunit of the KPC complex that acts as E3
CC ubiquitin-protein ligase. Required for poly-ubiquitination and
CC proteasome-mediated degradation of CDKN1B during G1 phase of the cell
CC cycle (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Component of the KPC complex composed of RNF123/KPC1 and
CC UBAC1/KPC2. Interacts with RNF123 via its N-terminal domain. Interacts
CC with the proteasome via its N-terminal domain (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR EMBL; AK157857; BAE34235.1; -; mRNA.
DR EMBL; BC021811; AAH21811.1; -; mRNA.
DR CCDS; CCDS15795.1; -.
DR RefSeq; NP_598596.2; NM_133835.2.
DR AlphaFoldDB; Q8VDI7; -.
DR SMR; Q8VDI7; -.
DR BioGRID; 221128; 7.
DR STRING; 10090.ENSMUSP00000040220; -.
DR iPTMnet; Q8VDI7; -.
DR PhosphoSitePlus; Q8VDI7; -.
DR EPD; Q8VDI7; -.
DR MaxQB; Q8VDI7; -.
DR PaxDb; Q8VDI7; -.
DR PeptideAtlas; Q8VDI7; -.
DR PRIDE; Q8VDI7; -.
DR ProteomicsDB; 297780; -.
DR Antibodypedia; 18666; 209 antibodies from 28 providers.
DR DNASU; 98766; -.
DR Ensembl; ENSMUST00000036509; ENSMUSP00000040220; ENSMUSG00000036352.
DR GeneID; 98766; -.
DR KEGG; mmu:98766; -.
DR UCSC; uc008itz.2; mouse.
DR CTD; 10422; -.
DR MGI; MGI:1920995; Ubac1.
DR VEuPathDB; HostDB:ENSMUSG00000036352; -.
DR eggNOG; ENOG502QQQ6; Eukaryota.
DR GeneTree; ENSGT00390000014658; -.
DR HOGENOM; CLU_035938_0_0_1; -.
DR InParanoid; Q8VDI7; -.
DR OMA; LHVCTME; -.
DR OrthoDB; 1163759at2759; -.
DR PhylomeDB; Q8VDI7; -.
DR TreeFam; TF324579; -.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 98766; 4 hits in 75 CRISPR screens.
DR ChiTaRS; Ubac1; mouse.
DR PRO; PR:Q8VDI7; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8VDI7; protein.
DR Bgee; ENSMUSG00000036352; Expressed in fetal liver hematopoietic progenitor cell and 267 other tissues.
DR ExpressionAtlas; Q8VDI7; baseline and differential.
DR Genevisible; Q8VDI7; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0000151; C:ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd14303; UBA1_KPC2; 1.
DR CDD; cd14304; UBA2_KPC2; 1.
DR InterPro; IPR006636; STI1_HS-bd.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR041926; UBA1_UBAC1.
DR InterPro; IPR041927; UBA2_UBAC1.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR Pfam; PF00627; UBA; 2.
DR SMART; SM00727; STI1; 1.
DR SMART; SM00165; UBA; 2.
DR SUPFAM; SSF46934; SSF46934; 2.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50030; UBA; 2.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Reference proteome; Repeat;
KW Ubl conjugation pathway.
FT CHAIN 1..409
FT /note="Ubiquitin-associated domain-containing protein 1"
FT /id="PRO_0000250450"
FT DOMAIN 14..98
FT /note="Ubiquitin-like"
FT DOMAIN 187..231
FT /note="UBA 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00212"
FT DOMAIN 292..332
FT /note="UBA 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00212"
FT DOMAIN 357..396
FT /note="STI1"
FT REGION 101..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 239..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..122
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..267
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9BSL1"
FT VARIANT 252
FT /note="A -> G (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT VARIANT 269
FT /note="V -> A (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT VARIANT 274
FT /note="S -> T (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
SQ SEQUENCE 409 AA; 45531 MW; 7CE7C9ACC5975231 CRC64;
MFVQEEKIFA GKVLRLHICA ADGAEWLEEA TEDTSVEKLK ESCLKHGAHG SLEDPKNVTH
HKLIHAASER VLSDSKTILE ENIQDQDVLL LIKKRVPSPL PKMADVSAEE KKKQEQKAPD
KDAILRATAN LPACSTDRTA VQTTMRDFQT ELRKILVSLI EVAQKLLALN PDAVELFKKA
NAMLDEDEDE RVDETALRQL TEMGFPESRA SKALRLNHMS VPQAMEWLIE HSEDPAIDTP
LPGHAAQAGA SAAATTSSTS SEAAVGTSVE DEESRDELTE IFKKIRRKKE FRADARAVIS
LMEMGFDEKE VIDALRVNNN QQNAACEWLL GDRKPSPEEL DQGIDPNSPL FQAILDNPVV
QLGLTNPKTL LAFEDMLENP LNSTQWMNDP ETGPVMLQIS RIFQTLNRT
