ID   UBACT_NITG3             Reviewed;          59 AA.
AC   M1YW29;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Prokaryotic ubiquitin-like protein UBact {ECO:0000303|PubMed:28087277};
GN   Name=ubact {ECO:0000303|PubMed:28087277};
GN   ORFNames=NITGR_170112 {ECO:0000312|EMBL:CCQ89855.1};
OS   Nitrospina gracilis (strain 3/211).
OC   Bacteria; Nitrospinae/Tectomicrobia group; Nitrospinae; Nitrospinia;
OC   Nitrospinales; Nitrospinaceae; Nitrospina.
OX   NCBI_TaxID=1266370;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3/211;
RX   PubMed=23439773; DOI=10.3389/fmicb.2013.00027;
RA   Luecker S., Nowka B., Rattei T., Spieck E., Daims H.;
RT   "The genome of Nitrospina gracilis illuminates the metabolism and evolution
RT   of the major marine nitrite oxidizer.";
RL   Front. Microbiol. 4:27-27(2013).
RN   [2]
RP   PREDICTED FUNCTION, AND DEAMIDATION AT GLN-59.
RC   STRAIN=3/211;
RX   PubMed=28087277; DOI=10.1016/j.bbrc.2017.01.037;
RA   Lehmann G., Udasin R.G., Livneh I., Ciechanover A.;
RT   "Identification of UBact, a ubiquitin-like protein, along with other
RT   homologous components of a conjugation system and the proteasome in
RT   different gram-negative bacteria.";
RL   Biochem. Biophys. Res. Commun. 483:946-950(2017).
CC   -!- FUNCTION: May function as a protein modifier covalently attached to
CC       lysine residues of substrate proteins. This may serve to target the
CC       modified proteins for degradation by proteasomes.
CC       {ECO:0000305|PubMed:28087277}.
CC   -!- PTM: May be modified by deamidation of its C-terminal glutamine to
CC       glutamate by the adjacently encoded deamidase. This could be a
CC       prerequisite to the subsequent conjugation, as shown in the other
CC       prokaryotic ubiquitin-like protein Pup. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-like protein UBact family.
CC       {ECO:0000255|HAMAP-Rule:MF_02133}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CAQJ01000019; CCQ89855.1; -; Genomic_DNA.
DR   RefSeq; WP_005006709.1; NZ_HG422173.1.
DR   AlphaFoldDB; M1YW29; -.
DR   SMR; M1YW29; -.
DR   EnsemblBacteria; CCQ89855; CCQ89855; NITGR_170112.
DR   HOGENOM; CLU_207029_0_0_0; -.
DR   OrthoDB; 2101563at2; -.
DR   Proteomes; UP000011704; Unassembled WGS sequence.
DR   GO; GO:0031386; F:protein tag; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_02133; UBact; 1.
DR   InterPro; IPR037543; UBact.
PE   1: Evidence at protein level;
KW   Isopeptide bond; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..59
FT                   /note="Prokaryotic ubiquitin-like protein UBact"
FT                   /id="PRO_0000441764"
FT   REGION          1..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         59
FT                   /note="Deamidated glutamine"
FT                   /evidence="ECO:0000305|PubMed:28087277"
FT   CROSSLNK        59
FT                   /note="Isoglutamyl lysine isopeptide (Gln-Lys) (interchain
FT                   with K-? in acceptor proteins)"
FT                   /evidence="ECO:0000305|PubMed:28087277"
SQ   SEQUENCE   59 AA;  6886 MW;  4FDC958C68332EF7 CRC64;
     MEMTDPLRRE EKKESSPDPK EESGPSRPDV SRPGRDSLLK RMKKVDPKQS EKYKQRTGQ