ID   UBACT_PEDPL             Reviewed;          56 AA.
AC   B9XEI9;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   14-APR-2009, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Prokaryotic ubiquitin-like protein UBact {ECO:0000303|PubMed:28087277};
GN   Name=ubact {ECO:0000303|PubMed:28087277};
GN   ORFNames=Cflav_PD4743 {ECO:0000312|EMBL:EEF61703.1};
OS   Pedosphaera parvula (strain Ellin514).
OC   Bacteria; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales;
OC   Verrucomicrobia subdivision 3; Pedosphaera.
OX   NCBI_TaxID=320771;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ellin514;
RX   PubMed=21460084; DOI=10.1128/jb.00299-11;
RA   Kant R., van Passel M.W., Sangwan P., Palva A., Lucas S., Copeland A.,
RA   Lapidus A., Glavina Del Rio T., Dalin E., Tice H., Bruce D., Goodwin L.,
RA   Pitluck S., Chertkov O., Larimer F.W., Land M.L., Hauser L., Brettin T.S.,
RA   Detter J.C., Han S., de Vos W.M., Janssen P.H., Smidt H.;
RT   "Genome sequence of 'Pedosphaera parvula' Ellin514, an aerobic
RT   Verrucomicrobial isolate from pasture soil.";
RL   J. Bacteriol. 193:2900-2901(2011).
RN   [2]
RP   PREDICTED FUNCTION, AND DEAMIDATION AT GLN-56.
RC   STRAIN=Ellin514;
RX   PubMed=28087277; DOI=10.1016/j.bbrc.2017.01.037;
RA   Lehmann G., Udasin R.G., Livneh I., Ciechanover A.;
RT   "Identification of UBact, a ubiquitin-like protein, along with other
RT   homologous components of a conjugation system and the proteasome in
RT   different gram-negative bacteria.";
RL   Biochem. Biophys. Res. Commun. 483:946-950(2017).
CC   -!- FUNCTION: May function as a protein modifier covalently attached to
CC       lysine residues of substrate proteins. This may serve to target the
CC       modified proteins for degradation by proteasomes.
CC       {ECO:0000305|PubMed:28087277}.
CC   -!- PTM: May be modified by deamidation of its C-terminal glutamine to
CC       glutamate by the adjacently encoded deamidase. This could be a
CC       prerequisite to the subsequent conjugation, as shown in the other
CC       prokaryotic ubiquitin-like protein Pup. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-like protein UBact family.
CC       {ECO:0000255|HAMAP-Rule:MF_02133}.
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DR   EMBL; ABOX02000008; EEF61703.1; -; Genomic_DNA.
DR   AlphaFoldDB; B9XEI9; -.
DR   SMR; B9XEI9; -.
DR   EnsemblBacteria; EEF61703; EEF61703; Cflav_PD4743.
DR   OMA; FRMEERR; -.
DR   Proteomes; UP000003688; Unassembled WGS sequence.
DR   GO; GO:0031386; F:protein tag; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_02133; UBact; 1.
DR   InterPro; IPR037543; UBact.
PE   1: Evidence at protein level;
KW   Isopeptide bond; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..56
FT                   /note="Prokaryotic ubiquitin-like protein UBact"
FT                   /id="PRO_0000441767"
FT   REGION          1..56
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        30..56
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         56
FT                   /note="Deamidated glutamine"
FT                   /evidence="ECO:0000305|PubMed:28087277"
FT   CROSSLNK        56
FT                   /note="Isoglutamyl lysine isopeptide (Gln-Lys) (interchain
FT                   with K-? in acceptor proteins)"
FT                   /evidence="ECO:0000305|PubMed:28087277"
SQ   SEQUENCE   56 AA;  6143 MW;  DEFE2DBC826D460B CRC64;
     MPDQAQKTRP VGPGPSGGGE GPGSPKVEKP NTEELLKRMR KVDPDQAKRY RQRTGQ