UBC12_CAEEL
ID UBC12_CAEEL Reviewed; 180 AA.
AC Q9XVK5;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=NEDD8-conjugating enzyme ubc-12;
DE EC=2.3.2.34;
DE AltName: Full=NEDD8 carrier protein;
DE AltName: Full=Ubiquitin-conjugating enzyme E2 12;
GN Name=ubc-12 {ECO:0000312|WormBase:R09B3.4};
GN ORFNames=R09B3.4 {ECO:0000312|WormBase:R09B3.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP IDENTIFICATION, NOMENCLATURE, DEVELOPMENTAL STAGE, FUNCTION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=10993680; DOI=10.1006/dbio.2000.9847;
RA Jones D., Candido E.P.M.;
RT "The NED-8 conjugating system in Caenorhabditis elegans is required for
RT embryogenesis and terminal differentiation of the hypodermis.";
RL Dev. Biol. 226:152-165(2000).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=21408209; DOI=10.1371/journal.pgen.1002010;
RA Nelson M.D., Zhou E., Kiontke K., Fradin H., Maldonado G., Martin D.,
RA Shah K., Fitch D.H.;
RT "A bow-tie genetic architecture for morphogenesis suggested by a genome-
RT wide RNAi screen in Caenorhabditis elegans.";
RL PLoS Genet. 7:E1002010-E1002010(2011).
CC -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the uba-3-ula-1
CC E1 complex and catalyzes its covalent attachment to other proteins
CC (PubMed:10993680). Plays a role in male tail tip morphogenesis
CC (PubMed:21408209). {ECO:0000269|PubMed:10993680,
CC ECO:0000269|PubMed:21408209}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine +
CC [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating
CC enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-
CC yl-L-cysteine.; EC=2.3.2.34;
CC -!- PATHWAY: Protein modification; protein neddylation.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21408209}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development
CC (PubMed:10993680). Highly expressed in hyp10 tail tip cells in early L4
CC larval stage males (PubMed:21408209). Expressed in hyp10 tail tip cells
CC prior to and during male tail tip retraction (PubMed:21408209).
CC {ECO:0000269|PubMed:10993680, ECO:0000269|PubMed:21408209}.
CC -!- DISRUPTION PHENOTYPE: Worms either arrest during embryonic development,
CC or show vulval eversion at the L4 stage and burst at the vulva during
CC the L4-to-adult molt (PubMed:10993680). Those who survive to the adult
CC stage display severe abnormalities in the structure of alae
CC (PubMed:10993680). RNAi-mediated knockdown results in severe male tail
CC tip defects (PubMed:21408209). {ECO:0000269|PubMed:10993680,
CC ECO:0000269|PubMed:21408209}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBC12
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; BX284601; CAB03238.1; -; Genomic_DNA.
DR PIR; T24069; T24069.
DR RefSeq; NP_493024.1; NM_060623.4.
DR AlphaFoldDB; Q9XVK5; -.
DR SMR; Q9XVK5; -.
DR BioGRID; 38475; 25.
DR STRING; 6239.R09B3.4; -.
DR EPD; Q9XVK5; -.
DR PaxDb; Q9XVK5; -.
DR PeptideAtlas; Q9XVK5; -.
DR EnsemblMetazoa; R09B3.4.1; R09B3.4.1; WBGene00006707.
DR GeneID; 173072; -.
DR KEGG; cel:CELE_R09B3.4; -.
DR UCSC; R09B3.4; c. elegans.
DR CTD; 173072; -.
DR WormBase; R09B3.4; CE16309; WBGene00006707; ubc-12.
DR eggNOG; KOG0420; Eukaryota.
DR GeneTree; ENSGT00940000154349; -.
DR HOGENOM; CLU_030988_6_4_1; -.
DR InParanoid; Q9XVK5; -.
DR OMA; PEYNNMP; -.
DR OrthoDB; 1302735at2759; -.
DR PhylomeDB; Q9XVK5; -.
DR UniPathway; UPA00885; -.
DR PRO; PR:Q9XVK5; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00006707; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0033503; C:HULC complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0019788; F:NEDD8 transferase activity; IDA:UniProtKB.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0016574; P:histone ubiquitination; IBA:GO_Central.
DR GO; GO:0043518; P:negative regulation of DNA damage response, signal transduction by p53 class mediator; IMP:UniProtKB.
DR GO; GO:0110039; P:positive regulation of nematode male tail tip morphogenesis; IMP:UniProtKB.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0045116; P:protein neddylation; IDA:UniProtKB.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Developmental protein; Nucleotide-binding;
KW Reference proteome; Transferase; Ubl conjugation pathway.
FT CHAIN 1..180
FT /note="NEDD8-conjugating enzyme ubc-12"
FT /id="PRO_0000082493"
FT DOMAIN 24..180
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 112
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 180 AA; 21101 MW; 1747CED555199C6A CRC64;
MFNLQKRING NNEDGRYLET RIAVRDKLLA QELQQLETAL RDQKQKLWHL EVPSTSCLHE
LELTVTPQEG IYRGGKFRFK ITVPPEYNNV PPVVKCLTKV WHPNINEDGS ICLSILRQNS
LDQYGWRPTR NLTDVVHGLV SLFNDLMDFN DALNIQAAQM WSQNRESFNH RVREYISRYC
