ID   UBC12_KLULA             Reviewed;         184 AA.
AC   Q6CSW8;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=NEDD8-conjugating enzyme UBC12;
DE            EC=2.3.2.34;
DE   AltName: Full=RUB1-conjugating enzyme;
DE   AltName: Full=Ubiquitin carrier protein 12;
GN   Name=UBC12; OrderedLocusNames=KLLA0C17248g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8/RUB1 from the UBA3-
CC       ULA1 E1 complex and catalyzes its covalent attachment to other
CC       proteins. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine +
CC         [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating
CC         enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-
CC         yl-L-cysteine.; EC=2.3.2.34;
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBC12
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; CR382123; CAH01822.1; -; Genomic_DNA.
DR   RefSeq; XP_452971.1; XM_452971.1.
DR   AlphaFoldDB; Q6CSW8; -.
DR   SMR; Q6CSW8; -.
DR   STRING; 28985.XP_452971.1; -.
DR   EnsemblFungi; CAH01822; CAH01822; KLLA0_C17248g.
DR   GeneID; 2892100; -.
DR   KEGG; kla:KLLA0_C17248g; -.
DR   eggNOG; KOG0420; Eukaryota.
DR   HOGENOM; CLU_030988_6_0_1; -.
DR   InParanoid; Q6CSW8; -.
DR   OMA; KESYFRG; -.
DR   UniPathway; UPA00885; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0019788; F:NEDD8 transferase activity; IEA:EnsemblFungi.
DR   GO; GO:0045116; P:protein neddylation; IEA:UniProtKB-UniPathway.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW   Ubl conjugation pathway.
FT   CHAIN           1..184
FT                   /note="NEDD8-conjugating enzyme UBC12"
FT                   /id="PRO_0000082498"
FT   DOMAIN          29..174
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   ACT_SITE        112
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   184 AA;  21166 MW;  B3105D8B1C705932 CRC64;
     MLKLRALAEA KAKAKEGQGQ ETTPQVIKPS LIRLKKDLEN IDLSEQICEY TTYLDENPMR
     VNLAIKPDIG YYAGGTYYFN VFIKDTYPME PPVVKCMHRI YHPNIDIDGN VCLNLLREDW
     TPALDIQSII IGILFLFHEP NGRDPLNKDA AKTLIEDPLR FENKVNHSLR GNNIDGVWYD
     KIIY