ID   UBC12_YARLI             Reviewed;         179 AA.
AC   Q6C9W0;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=NEDD8-conjugating enzyme UBC12;
DE            EC=2.3.2.34;
DE   AltName: Full=RUB1-conjugating enzyme;
DE   AltName: Full=Ubiquitin carrier protein 12;
GN   Name=UBC12; OrderedLocusNames=YALI0D07890g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8/RUB1 from the UBA3-
CC       ULA1 E1 complex and catalyzes its covalent attachment to other
CC       proteins. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine +
CC         [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating
CC         enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-
CC         yl-L-cysteine.; EC=2.3.2.34;
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBC12
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR382130; CAG80740.1; -; Genomic_DNA.
DR   RefSeq; XP_502552.1; XM_502552.1.
DR   AlphaFoldDB; Q6C9W0; -.
DR   SMR; Q6C9W0; -.
DR   STRING; 4952.CAG80740; -.
DR   PRIDE; Q6C9W0; -.
DR   EnsemblFungi; CAG80740; CAG80740; YALI0_D07890g.
DR   GeneID; 2910945; -.
DR   KEGG; yli:YALI0D07890g; -.
DR   VEuPathDB; FungiDB:YALI0_D07890g; -.
DR   HOGENOM; CLU_030988_6_0_1; -.
DR   InParanoid; Q6C9W0; -.
DR   OMA; GYPHDAP; -.
DR   UniPathway; UPA00885; -.
DR   Proteomes; UP000001300; Chromosome D.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0019788; F:NEDD8 transferase activity; IBA:GO_Central.
DR   GO; GO:0045116; P:protein neddylation; IBA:GO_Central.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW   Ubl conjugation pathway.
FT   CHAIN           1..179
FT                   /note="NEDD8-conjugating enzyme UBC12"
FT                   /id="PRO_0000082500"
FT   DOMAIN          24..168
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   ACT_SITE        106
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   179 AA;  20271 MW;  38434BAC88FF21D5 CRC64;
     MLKIWSMKEK QAAEGGSAKK KVTAAQLRVQ KDLSELSLPS TMKTHFPSAE DIMNFELTVR
     PDEGFYQGGE FRFSFYVNPN FPHEPPKVKC LQKVYHPNID LEGNVCLNIL REDWKPVLSL
     NAVMIGLQYL FLEPNASDPL NKDAAHQMTA NREEFKRNVK HSMAGGSVAG ERFDCVLIK