ID   UBC1_COLGL              Reviewed;         147 AA.
AC   O74196;
DT   17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2-16 kDa;
DE            EC=2.3.2.23;
DE   AltName: Full=Colletotrichum hard-surface-induced protein 1;
DE   AltName: Full=E2 ubiquitin-conjugating enzyme 1;
DE   AltName: Full=Ubiquitin carrier protein;
DE   AltName: Full=Ubiquitin-protein ligase;
GN   Name=UBC1; Synonyms=CHIP1;
OS   Colletotrichum gloeosporioides (Anthracnose fungus) (Glomerella cingulata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum;
OC   Colletotrichum gloeosporioides species complex.
OX   NCBI_TaxID=474922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Conidium;
RX   PubMed=9658002; DOI=10.1128/jb.180.14.3592-3597.1998;
RA   Liu Z.-M., Kolattukudy P.E.;
RT   "Identification of a gene product induced by hard-surface contact of
RT   Colletotrichum gloeosporioides conidia as a ubiquitin-conjugating enzyme by
RT   yeast complementation.";
RL   J. Bacteriol. 180:3592-3597(1998).
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. May also mediate selective proteolysis pathways.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC         [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC         activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC         ProRule:PRU10133};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the conidium during the process of
CC       appressorium formation.
CC   -!- INDUCTION: By hard-surface contact. Ethylene and the host surface wax
CC       enhance this induction which is essential for appressorium formation.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; AF030296; AAC39499.1; -; mRNA.
DR   AlphaFoldDB; O74196; -.
DR   SMR; O74196; -.
DR   UniPathway; UPA00143; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IEA:UniProtKB-EC.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Nucleotide-binding; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..147
FT                   /note="Ubiquitin-conjugating enzyme E2-16 kDa"
FT                   /id="PRO_0000082542"
FT   DOMAIN          1..147
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   ACT_SITE        107
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   147 AA;  16528 MW;  6B65B0BE2F37D528 CRC64;
     MAFKRINKEL TDLGRDPPSS CSAGPVGEDL FHWQATIMGP GDSPYSGGVF FLAIHFPTDY
     PFKPPKVNFT TRIYHPNINS NGSICLDILR DQWSPALTIS KVLLSICSML TDPNPDEPLV
     PEIAHVYKTD RARYEATARE WTRKYAI