UBC1_MAGO7
ID UBC1_MAGO7 Reviewed; 147 AA.
AC Q9UVR2; A4R868; G4N6D7;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Ubiquitin-conjugating enzyme E2-16 kDa;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme 1;
DE AltName: Full=Ubiquitin carrier protein;
DE AltName: Full=Ubiquitin-protein ligase;
GN Name=UBC1; ORFNames=MGG_06562;
OS Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS fungus) (Pyricularia oryzae).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX NCBI_TaxID=242507;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kim Y.-K., Kolattukudy P.E.;
RT "Identification of a ubiquitin-conjugating enzyme of the rice blast fungus
RT Magnaporthe grisea.";
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX PubMed=15846337; DOI=10.1038/nature03449;
RA Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL Nature 434:980-986(2005).
CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC proteins. {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; AF161722; AAD55983.1; -; Genomic_DNA.
DR EMBL; CM001234; EHA50659.1; -; Genomic_DNA.
DR RefSeq; XP_003716978.1; XM_003716930.1.
DR AlphaFoldDB; Q9UVR2; -.
DR SMR; Q9UVR2; -.
DR STRING; 318829.MGG_06562T0; -.
DR PRIDE; Q9UVR2; -.
DR EnsemblFungi; MGG_06562T0; MGG_06562T0; MGG_06562.
DR GeneID; 2684717; -.
DR KEGG; mgr:MGG_06562; -.
DR VEuPathDB; FungiDB:MGG_06562; -.
DR eggNOG; KOG0417; Eukaryota.
DR HOGENOM; CLU_030988_13_3_1; -.
DR InParanoid; Q9UVR2; -.
DR OMA; VHFTTRI; -.
DR OrthoDB; 1337945at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000009058; Chromosome 4.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IEA:UniProtKB-EC.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..147
FT /note="Ubiquitin-conjugating enzyme E2-16 kDa"
FT /id="PRO_0000082543"
FT DOMAIN 1..147
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 107
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
FT CONFLICT 128
FT /note="K -> R (in Ref. 1; AAD55983)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="S -> A (in Ref. 1; AAD55983)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="R -> P (in Ref. 1; AAD55983)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 147 AA; 16438 MW; C7737C95FB7F61F7 CRC64;
MALKRINKEL TDLGRDPPSS CSAGPVGEDL FHWQATIMGP SDSPYAGGVF FLAIHFPTDY
PFKPPKVNFT TRIYHPNINS NGSICLDILR DQWSPALTIS KVLLSICSML TDPNPDDPLV
PEIAHVYKTA RAQYESTARE WTRKYAI
