ID   UBC21_CAEEL             Reviewed;         214 AA.
AC   P52484;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   25-JAN-2012, sequence version 3.
DT   03-AUG-2022, entry version 142.
DE   RecName: Full=Probable ubiquitin-conjugating enzyme E2 21;
DE            EC=2.3.2.23;
DE   AltName: Full=E2 ubiquitin-conjugating enzyme 21;
DE   AltName: Full=Ubiquitin carrier protein 21;
DE   AltName: Full=Ubiquitin-protein ligase 21;
GN   Name=ubc-21; ORFNames=C06E2.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC         [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC         activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC         ProRule:PRU10133};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR   EMBL; FO080381; CCD63324.1; -; Genomic_DNA.
DR   PIR; T15432; T15432.
DR   RefSeq; NP_509502.3; NM_077101.3.
DR   AlphaFoldDB; P52484; -.
DR   SMR; P52484; -.
DR   BioGRID; 47176; 1.
DR   STRING; 6239.C06E2.3; -.
DR   PaxDb; P52484; -.
DR   PeptideAtlas; P52484; -.
DR   EnsemblMetazoa; C06E2.3.1; C06E2.3.1; WBGene00006716.
DR   GeneID; 182321; -.
DR   KEGG; cel:CELE_C06E2.3; -.
DR   UCSC; C06E2.3; c. elegans.
DR   CTD; 182321; -.
DR   WormBase; C06E2.3; CE46306; WBGene00006716; ubc-21.
DR   eggNOG; KOG0418; Eukaryota.
DR   HOGENOM; CLU_030988_13_1_1; -.
DR   InParanoid; P52484; -.
DR   OMA; WTNLRGE; -.
DR   OrthoDB; 1418652at2759; -.
DR   PhylomeDB; P52484; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:P52484; -.
DR   Proteomes; UP000001940; Chromosome X.
DR   Bgee; WBGene00006716; Expressed in larva.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; -; 1.
DR   InterPro; IPR015940; UBA.
DR   InterPro; IPR009060; UBA-like_sf.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   Pfam; PF00179; UQ_con; 1.
DR   SUPFAM; SSF46934; SSF46934; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   PROSITE; PS50030; UBA; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW   Ubl conjugation pathway.
FT   CHAIN           1..214
FT                   /note="Probable ubiquitin-conjugating enzyme E2 21"
FT                   /id="PRO_0000082518"
FT   DOMAIN          21..168
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   DOMAIN          172..214
FT                   /note="UBA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00212"
FT   ACT_SITE        106
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   214 AA;  24204 MW;  F061FFE2FB9EF130 CRC64;
     MSVSKLNKMQ FSDKMSNLAL ARVTRKCKEV ANASDITEAG IHVEIKENNL MDIKGFIKGP
     EGTPYAGGTF EIKVDIPEHY PFEPPKAKFV TRIWHPNISS QTGTICLDIL KDKWTASLTL
     RTVLLSLQAM LCSPEPSDPQ DAVVAKQFIN NYPMFTATAV YWTSYFANSK KDVEPDFNRK
     VGRLIEMGIR ETEAIVYLSC NNWKLEQALQ FIFD