UBC2_WHEAT
ID UBC2_WHEAT Reviewed; 152 AA.
AC P25866;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 2;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme 2;
DE AltName: Full=Ubiquitin carrier protein 2;
DE AltName: Full=Ubiquitin-conjugating enzyme E2-16 kDa;
DE AltName: Full=Ubiquitin-protein ligase 2;
GN Name=UBC2; Synonyms=UBC1;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND MUTAGENESIS OF CYS-88.
RC STRAIN=cv. Augusta;
RX PubMed=1660887; DOI=10.1016/s0021-9258(18)54365-9;
RA Sullivan M.L., Vierstra R.D.;
RT "Cloning of a 16-kDa ubiquitin carrier protein from wheat and Arabidopsis
RT thaliana. Identification of functional domains by in vitro mutagenesis.";
RL J. Biol. Chem. 266:23878-23885(1991).
CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC proteins.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; M62720; AAA34310.1; -; mRNA.
DR AlphaFoldDB; P25866; -.
DR SMR; P25866; -.
DR STRING; 4565.Traes_5BL_D7BE0A670.1; -.
DR EnsemblPlants; TraesCAD_scaffold_006725_01G000300.1; TraesCAD_scaffold_006725_01G000300.1; TraesCAD_scaffold_006725_01G000300.
DR EnsemblPlants; TraesCAD_scaffold_015453_01G000200.1; TraesCAD_scaffold_015453_01G000200.1; TraesCAD_scaffold_015453_01G000200.
DR EnsemblPlants; TraesCAD_scaffold_019556_01G000100.1; TraesCAD_scaffold_019556_01G000100.1; TraesCAD_scaffold_019556_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_003163_01G001000.1; TraesCLE_scaffold_003163_01G001000.1; TraesCLE_scaffold_003163_01G001000.
DR EnsemblPlants; TraesCLE_scaffold_068154_01G000200.1; TraesCLE_scaffold_068154_01G000200.1; TraesCLE_scaffold_068154_01G000200.
DR EnsemblPlants; TraesCLE_scaffold_069524_01G000200.1; TraesCLE_scaffold_069524_01G000200.1; TraesCLE_scaffold_069524_01G000200.
DR EnsemblPlants; TraesCS5A02G429000.1; TraesCS5A02G429000.1; TraesCS5A02G429000.
DR EnsemblPlants; TraesCS5B02G431100.1; TraesCS5B02G431100.1; TraesCS5B02G431100.
DR EnsemblPlants; TraesCS5D02G437100.1; TraesCS5D02G437100.1; TraesCS5D02G437100.
DR EnsemblPlants; TraesPAR_scaffold_001164_01G000400.1; TraesPAR_scaffold_001164_01G000400.1; TraesPAR_scaffold_001164_01G000400.
DR EnsemblPlants; TraesPAR_scaffold_005640_01G001000.1; TraesPAR_scaffold_005640_01G001000.1; TraesPAR_scaffold_005640_01G001000.
DR EnsemblPlants; TraesPAR_scaffold_082590_01G000100.1; TraesPAR_scaffold_082590_01G000100.1; TraesPAR_scaffold_082590_01G000100.
DR EnsemblPlants; TraesROB_scaffold_044854_01G000200.1; TraesROB_scaffold_044854_01G000200.1; TraesROB_scaffold_044854_01G000200.
DR EnsemblPlants; TraesROB_scaffold_071098_01G000200.1; TraesROB_scaffold_071098_01G000200.1; TraesROB_scaffold_071098_01G000200.
DR EnsemblPlants; TraesROB_scaffold_132899_01G000100.1; TraesROB_scaffold_132899_01G000100.1; TraesROB_scaffold_132899_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_005805_01G001000.1; TraesWEE_scaffold_005805_01G001000.1; TraesWEE_scaffold_005805_01G001000.
DR EnsemblPlants; TraesWEE_scaffold_044390_01G000200.1; TraesWEE_scaffold_044390_01G000200.1; TraesWEE_scaffold_044390_01G000200.
DR EnsemblPlants; TraesWEE_scaffold_086268_01G000200.1; TraesWEE_scaffold_086268_01G000200.1; TraesWEE_scaffold_086268_01G000200.
DR Gramene; TraesCAD_scaffold_006725_01G000300.1; TraesCAD_scaffold_006725_01G000300.1; TraesCAD_scaffold_006725_01G000300.
DR Gramene; TraesCAD_scaffold_015453_01G000200.1; TraesCAD_scaffold_015453_01G000200.1; TraesCAD_scaffold_015453_01G000200.
DR Gramene; TraesCAD_scaffold_019556_01G000100.1; TraesCAD_scaffold_019556_01G000100.1; TraesCAD_scaffold_019556_01G000100.
DR Gramene; TraesCLE_scaffold_003163_01G001000.1; TraesCLE_scaffold_003163_01G001000.1; TraesCLE_scaffold_003163_01G001000.
DR Gramene; TraesCLE_scaffold_068154_01G000200.1; TraesCLE_scaffold_068154_01G000200.1; TraesCLE_scaffold_068154_01G000200.
DR Gramene; TraesCLE_scaffold_069524_01G000200.1; TraesCLE_scaffold_069524_01G000200.1; TraesCLE_scaffold_069524_01G000200.
DR Gramene; TraesCS5A02G429000.1; TraesCS5A02G429000.1; TraesCS5A02G429000.
DR Gramene; TraesCS5B02G431100.1; TraesCS5B02G431100.1; TraesCS5B02G431100.
DR Gramene; TraesCS5D02G437100.1; TraesCS5D02G437100.1; TraesCS5D02G437100.
DR Gramene; TraesPAR_scaffold_001164_01G000400.1; TraesPAR_scaffold_001164_01G000400.1; TraesPAR_scaffold_001164_01G000400.
DR Gramene; TraesPAR_scaffold_005640_01G001000.1; TraesPAR_scaffold_005640_01G001000.1; TraesPAR_scaffold_005640_01G001000.
DR Gramene; TraesPAR_scaffold_082590_01G000100.1; TraesPAR_scaffold_082590_01G000100.1; TraesPAR_scaffold_082590_01G000100.
DR Gramene; TraesROB_scaffold_044854_01G000200.1; TraesROB_scaffold_044854_01G000200.1; TraesROB_scaffold_044854_01G000200.
DR Gramene; TraesROB_scaffold_071098_01G000200.1; TraesROB_scaffold_071098_01G000200.1; TraesROB_scaffold_071098_01G000200.
DR Gramene; TraesROB_scaffold_132899_01G000100.1; TraesROB_scaffold_132899_01G000100.1; TraesROB_scaffold_132899_01G000100.
DR Gramene; TraesWEE_scaffold_005805_01G001000.1; TraesWEE_scaffold_005805_01G001000.1; TraesWEE_scaffold_005805_01G001000.
DR Gramene; TraesWEE_scaffold_044390_01G000200.1; TraesWEE_scaffold_044390_01G000200.1; TraesWEE_scaffold_044390_01G000200.
DR Gramene; TraesWEE_scaffold_086268_01G000200.1; TraesWEE_scaffold_086268_01G000200.1; TraesWEE_scaffold_086268_01G000200.
DR eggNOG; KOG0419; Eukaryota.
DR OMA; VHFVSRM; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; P25866; baseline and differential.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0016574; P:histone ubiquitination; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..152
FT /note="Ubiquitin-conjugating enzyme E2 2"
FT /id="PRO_0000082573"
FT DOMAIN 4..150
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 88
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT MUTAGEN 88
FT /note="C->S: Loss of activity."
FT /evidence="ECO:0000269|PubMed:1660887"
SQ SEQUENCE 152 AA; 17301 MW; F13AA5439EB4C8AB CRC64;
MSTPARKRLM RDFKRLQQDP PAGISGAPHD NNITLWNAVI FGPDDTPWDG GTFKLTLQFT
EDYPNKPPTV RFVSRMFHPN IYADGSICLD ILQNQWSPIY DVAAILTSIQ SLLCDPNPNS
PANSEAARMY SENKREYNRK VREVVEQSWT AD
