UBC4_CANAX
ID UBC4_CANAX Reviewed; 147 AA.
AC P43102;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 4;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme 4;
DE AltName: Full=Ubiquitin carrier protein 4;
DE AltName: Full=Ubiquitin-protein ligase 4;
GN Name=UBC4;
OS Candida albicans (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=5476;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7698660; DOI=10.1016/0378-1119(94)00926-j;
RA Damagnez V., Rolfe M., Cottarel G.;
RT "Schizosaccharomyces pombe and Candida albicans cDNA homologues of the
RT Saccharomyces cerevisiae UBC4 gene.";
RL Gene 155:137-138(1995).
CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC proteins. Mediates the selective degradation of short-lived and
CC abnormal proteins. Mediates ubiquitination of PEX5.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; L37383; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; P43102; -.
DR SMR; P43102; -.
DR VEuPathDB; FungiDB:CAWG_02261; -.
DR VEuPathDB; FungiDB:CR_09970W_A; -.
DR UniPathway; UPA00143; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0070628; F:proteasome binding; IEA:EnsemblFungi.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR GO; GO:0000209; P:protein polyubiquitination; ISS:UniProtKB.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Nucleotide-binding; Transferase; Ubl conjugation pathway.
FT CHAIN 1..147
FT /note="Ubiquitin-conjugating enzyme E2 4"
FT /id="PRO_0000082544"
FT DOMAIN 1..147
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 85
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 147 AA; 16340 MW; A86557BE37375EB9 CRC64;
MSLKRINKEL SDLGRDPPSS CSAGPVGDDL YHWQASIMGP PDSPYAGGVF FLSIHFPTDY
PLKPPKIALT TKIYHPNINS NGNICLDILK DQWSPALTIS KVLLSICSLL TDANPDDPLV
PEIAHIYKQD RKKYEATAKE WTKKYAV
