UBC4_SOLLC
ID UBC4_SOLLC Reviewed; 148 AA.
AC P35135;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Ubiquitin-conjugating enzyme E2-17 kDa;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme;
DE AltName: Full=Ubiquitin carrier protein;
DE AltName: Full=Ubiquitin-protein ligase;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Root;
RA Bird D.M., Wilson M.A.;
RT "Expression of an ubiquitin carrier protein gene in tomato root cells
RT infected with the nematode Meloidogyne incognita.";
RL Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC proteins. Mediates the selective degradation of short-lived and
CC abnormal proteins. {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; L23762; AAA34125.1; -; mRNA.
DR RefSeq; NP_001234247.1; NM_001247318.2.
DR RefSeq; NP_001294911.1; NM_001307982.1.
DR RefSeq; NP_001294913.1; NM_001307984.1.
DR RefSeq; XP_019069675.1; XM_019214130.1.
DR AlphaFoldDB; P35135; -.
DR SMR; P35135; -.
DR STRING; 4081.Solyc06g007510.2.1; -.
DR PaxDb; P35135; -.
DR EnsemblPlants; Solyc06g007510.3.1; Solyc06g007510.3.1; Solyc06g007510.3.
DR EnsemblPlants; Solyc06g082600.3.1; Solyc06g082600.3.1; Solyc06g082600.3.
DR GeneID; 100301932; -.
DR GeneID; 101249087; -.
DR Gramene; Solyc06g007510.3.1; Solyc06g007510.3.1; Solyc06g007510.3.
DR Gramene; Solyc06g082600.3.1; Solyc06g082600.3.1; Solyc06g082600.3.
DR KEGG; sly:100301932; -.
DR KEGG; sly:101249087; -.
DR eggNOG; KOG0417; Eukaryota.
DR HOGENOM; CLU_030988_13_3_1; -.
DR InParanoid; P35135; -.
DR OMA; NELPMAS; -.
DR OrthoDB; 1337945at2759; -.
DR PhylomeDB; P35135; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000004994; Chromosome 6.
DR ExpressionAtlas; P35135; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..148
FT /note="Ubiquitin-conjugating enzyme E2-17 kDa"
FT /id="PRO_0000082580"
FT DOMAIN 1..147
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 85
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 148 AA; 16522 MW; 4247FF721B98059F CRC64;
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP TDSPYAGGVF LVSIHFPPDY
PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS KVLLSICSLL TDPNPDDPLV
PEIAHMYKTD RAKYETTARS WTQKYAMG
