UBC4_WHEAT
ID UBC4_WHEAT Reviewed; 184 AA.
AC P16577;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Ubiquitin-conjugating enzyme E2-23 kDa;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme;
DE AltName: Full=Ubiquitin carrier protein;
DE AltName: Full=Ubiquitin-protein ligase;
GN Name=UBC4;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Augusta;
RX PubMed=2557633; DOI=10.1073/pnas.86.24.9861;
RA Sullivan M.L., Vierstra R.D.;
RT "A ubiquitin carrier protein from wheat germ is structurally and
RT functionally similar to the yeast DNA repair enzyme encoded by RAD6.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:9861-9865(1989).
RN [2]
RP MUTAGENESIS OF CYS-85.
RX PubMed=1660887; DOI=10.1016/s0021-9258(18)54365-9;
RA Sullivan M.L., Vierstra R.D.;
RT "Cloning of a 16-kDa ubiquitin carrier protein from wheat and Arabidopsis
RT thaliana. Identification of functional domains by in vitro mutagenesis.";
RL J. Biol. Chem. 266:23878-23885(1991).
CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC proteins.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; M28059; AAA34309.1; -; mRNA.
DR PIR; A34506; A34506.
DR AlphaFoldDB; P16577; -.
DR SMR; P16577; -.
DR STRING; 4565.Traes_5DL_51681402F.1; -.
DR PRIDE; P16577; -.
DR EnsemblPlants; TraesCAD_scaffold_095608_01G000100.1; TraesCAD_scaffold_095608_01G000100.1; TraesCAD_scaffold_095608_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_012902_01G000500.1; TraesCLE_scaffold_012902_01G000500.1; TraesCLE_scaffold_012902_01G000500.
DR EnsemblPlants; TraesCS4A02G313600.1; TraesCS4A02G313600.1; TraesCS4A02G313600.
DR EnsemblPlants; TraesPAR_scaffold_128789_01G000300.1; TraesPAR_scaffold_128789_01G000300.1; TraesPAR_scaffold_128789_01G000300.
DR EnsemblPlants; TraesROB_scaffold_016095_01G000100.1; TraesROB_scaffold_016095_01G000100.1; TraesROB_scaffold_016095_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_010029_01G000500.1; TraesWEE_scaffold_010029_01G000500.1; TraesWEE_scaffold_010029_01G000500.
DR Gramene; TraesCAD_scaffold_095608_01G000100.1; TraesCAD_scaffold_095608_01G000100.1; TraesCAD_scaffold_095608_01G000100.
DR Gramene; TraesCLE_scaffold_012902_01G000500.1; TraesCLE_scaffold_012902_01G000500.1; TraesCLE_scaffold_012902_01G000500.
DR Gramene; TraesCS4A02G313600.1; TraesCS4A02G313600.1; TraesCS4A02G313600.
DR Gramene; TraesPAR_scaffold_128789_01G000300.1; TraesPAR_scaffold_128789_01G000300.1; TraesPAR_scaffold_128789_01G000300.
DR Gramene; TraesROB_scaffold_016095_01G000100.1; TraesROB_scaffold_016095_01G000100.1; TraesROB_scaffold_016095_01G000100.
DR Gramene; TraesWEE_scaffold_010029_01G000500.1; TraesWEE_scaffold_010029_01G000500.1; TraesWEE_scaffold_010029_01G000500.
DR eggNOG; KOG0416; Eukaryota.
DR OMA; LMMSDWK; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000019116; Unplaced.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..184
FT /note="Ubiquitin-conjugating enzyme E2-23 kDa"
FT /id="PRO_0000082581"
FT DOMAIN 1..148
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT REGION 146..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..171
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 85
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT MUTAGEN 85
FT /note="C->S: Loss of activity."
FT /evidence="ECO:0000269|PubMed:1660887"
SQ SEQUENCE 184 AA; 21126 MW; AA479C4E42C893B7 CRC64;
MSSPSKRREM DLMKLMMSDY KVDMINDGMH EFFVHFHGPK DSIYQGGVWK VRVELTEAYP
YKSPSIGFTN KIYHPNVDEM SGSVCLDVIN QTWSPMFDLV NIFEVFLPQL LLYPNPSDPL
NGEAASLMMR DKNAYENKVK EYCERYAKPE DISPEEEEEE SDEELSDAEG YDSGDEAIMG
HADP
