UBE2A_MOUSE
ID UBE2A_MOUSE Reviewed; 152 AA.
AC Q9Z255;
DT 04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 A;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme A;
DE AltName: Full=RAD6 homolog A;
DE Short=HR6A;
DE Short=mHR6A;
DE AltName: Full=Ubiquitin carrier protein A;
DE AltName: Full=Ubiquitin-protein ligase A;
GN Name=Ube2a; Synonyms=Rad6a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BCBA; TISSUE=Brain;
RA Roest H.P., van Klaveren J., Koken M.H.M., Vermey M., van Cappellen W.A.,
RA Baarends W.M., Hoogerbrugge J.W., Bootsma D., Hoeijmakers J.H.J.,
RA Grootegoed J.A., de Wit J.;
RT "Isolation of mHR6A, a gene highly homologous to the male fertility gene
RT mHR6B.";
RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Skeletal muscle;
RA Kwon Y.T., Varshavsky A.;
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH RFPL4A AND CCNB1.
RX PubMed=12525704; DOI=10.1073/pnas.0234474100;
RA Suzumori N., Burns K.H., Yan W., Matzuk M.M.;
RT "RFPL4 interacts with oocyte proteins of the ubiquitin-proteasome
RT degradation pathway.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:550-555(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Lung, Pancreas, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its
CC covalent attachment to other proteins. In association with the E3
CC enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription
CC regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-
CC 120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic
CC transcriptional activation, elongation by RNA polymerase II, telomeric
CC silencing, and is also a prerequisite for H3K4me and H3K79me formation.
CC In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked
CC polyubiquitination. Required for postreplication repair of UV-damaged
CC DNA. {ECO:0000250|UniProtKB:P49459}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000250|UniProtKB:P49459, ECO:0000255|PROSITE-
CC ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SUBUNIT: Interacts with RAD18 and WAC (By similarity). Interacts with
CC RFPL4A and CCNB1 (PubMed:12525704). {ECO:0000250|UniProtKB:P49459,
CC ECO:0000269|PubMed:12525704}.
CC -!- PTM: Phosphorylation at Ser-120 by CDK9 increases activity towards
CC histone H2B. {ECO:0000250|UniProtKB:P49459}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; AF089812; AAC64563.1; -; mRNA.
DR EMBL; AF383148; AAK62984.1; -; mRNA.
DR EMBL; BC026053; AAH26053.1; -; mRNA.
DR CCDS; CCDS30064.1; -.
DR RefSeq; NP_062642.1; NM_019668.4.
DR AlphaFoldDB; Q9Z255; -.
DR SMR; Q9Z255; -.
DR BioGRID; 204414; 9.
DR STRING; 10090.ENSMUSP00000016452; -.
DR iPTMnet; Q9Z255; -.
DR PhosphoSitePlus; Q9Z255; -.
DR SwissPalm; Q9Z255; -.
DR EPD; Q9Z255; -.
DR MaxQB; Q9Z255; -.
DR PaxDb; Q9Z255; -.
DR PRIDE; Q9Z255; -.
DR ProteomicsDB; 298179; -.
DR Antibodypedia; 29799; 209 antibodies from 28 providers.
DR DNASU; 22209; -.
DR Ensembl; ENSMUST00000016452; ENSMUSP00000016452; ENSMUSG00000016308.
DR GeneID; 22209; -.
DR KEGG; mmu:22209; -.
DR UCSC; uc009sxv.1; mouse.
DR CTD; 7319; -.
DR MGI; MGI:102959; Ube2a.
DR VEuPathDB; HostDB:ENSMUSG00000016308; -.
DR eggNOG; KOG0419; Eukaryota.
DR GeneTree; ENSGT00940000155075; -.
DR HOGENOM; CLU_030988_10_2_1; -.
DR InParanoid; Q9Z255; -.
DR OMA; DHKSQYI; -.
DR OrthoDB; 1292821at2759; -.
DR PhylomeDB; Q9Z255; -.
DR TreeFam; TF101128; -.
DR Reactome; R-MMU-8866652; Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
DR Reactome; R-MMU-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 22209; 17 hits in 113 CRISPR screens.
DR ChiTaRS; Ube2a; mouse.
DR PRO; PR:Q9Z255; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q9Z255; protein.
DR Bgee; ENSMUSG00000016308; Expressed in placenta labyrinth and 263 other tissues.
DR ExpressionAtlas; Q9Z255; baseline and differential.
DR Genevisible; Q9Z255; MM.
DR GO; GO:0000785; C:chromatin; IDA:MGI.
DR GO; GO:0033503; C:HULC complex; ISS:UniProtKB.
DR GO; GO:0001741; C:XY body; IDA:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IGI:MGI.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IDA:MGI.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR GO; GO:0001835; P:blastocyst hatching; IMP:MGI.
DR GO; GO:0006281; P:DNA repair; ISO:MGI.
DR GO; GO:0033522; P:histone H2A ubiquitination; ISO:MGI.
DR GO; GO:0016574; P:histone ubiquitination; IBA:GO_Central.
DR GO; GO:0001701; P:in utero embryonic development; IGI:MGI.
DR GO; GO:0060135; P:maternal process involved in female pregnancy; IMP:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0051865; P:protein autoubiquitination; ISO:MGI.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IDA:MGI.
DR GO; GO:0009411; P:response to UV; ISO:MGI.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA damage; DNA repair; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Transferase; Ubl conjugation pathway.
FT CHAIN 1..152
FT /note="Ubiquitin-conjugating enzyme E2 A"
FT /id="PRO_0000082446"
FT DOMAIN 4..150
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 88
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
FT MOD_RES 120
FT /note="Phosphoserine; by CDK9"
FT /evidence="ECO:0000250|UniProtKB:P49459"
SQ SEQUENCE 152 AA; 17315 MW; 0AAEB5B7770E47E2 CRC64;
MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT
EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS
PANSQAAQLY QENKREYEKR VSAIVEQSWR DC
