UBE2B_RABIT
ID UBE2B_RABIT Reviewed; 152 AA.
AC P63148; P23567; Q9D0J6;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 B;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme B;
DE AltName: Full=RAD6 homolog B;
DE Short=HR6B;
DE AltName: Full=Ubiquitin carrier protein B;
DE AltName: Full=Ubiquitin-conjugating enzyme E2(14k);
DE AltName: Full=Ubiquitin-protein ligase B;
GN Name=UBE2B; Synonyms=RAD6B;
OS Oryctolagus cuniculus (Rabbit).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX NCBI_TaxID=9986;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1313008; DOI=10.1016/s0021-9258(19)50455-0;
RA Wing S.S., Dumas F., Banville D.;
RT "A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin-
RT dependent proteolysis (E214k) is homologous to the yeast DNA repair gene
RT RAD6.";
RL J. Biol. Chem. 267:6495-6501(1992).
CC -!- FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its
CC covalent attachment to other proteins. In association with the E3
CC enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription
CC regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-
CC 120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic
CC transcriptional activation, elongation by RNA polymerase II, telomeric
CC silencing, and is also a prerequisite for H3K4me and H3K79me formation.
CC In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked
CC polyubiquitination. Required for postreplication repair of UV-damaged
CC DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18
CC ubiquitin ligase complex involved in mono-ubiquitination of DNA-
CC associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
CC {ECO:0000250|UniProtKB:P63146}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000250|UniProtKB:P63146, ECO:0000255|PROSITE-
CC ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SUBUNIT: Interacts with RAD18, UBR2 and WAC.
CC {ECO:0000250|UniProtKB:P63146}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P63149}.
CC Nucleus {ECO:0000250|UniProtKB:P63149}. Note=In peripheral neurons,
CC expressed both at the plasma membrane and in nuclei.
CC {ECO:0000250|UniProtKB:P63149}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M62387; AAA31492.1; -; mRNA.
DR PIR; A42416; A42416.
DR RefSeq; NP_001075765.1; NM_001082296.1.
DR AlphaFoldDB; P63148; -.
DR BMRB; P63148; -.
DR SMR; P63148; -.
DR BioGRID; 1172155; 1.
DR IntAct; P63148; 2.
DR STRING; 9986.ENSOCUP00000013548; -.
DR Ensembl; ENSOCUT00000015769; ENSOCUP00000013548; ENSOCUG00000015774.
DR GeneID; 100009132; -.
DR KEGG; ocu:100009132; -.
DR CTD; 7320; -.
DR eggNOG; KOG0419; Eukaryota.
DR GeneTree; ENSGT00940000156580; -.
DR HOGENOM; CLU_030988_10_2_1; -.
DR InParanoid; P63148; -.
DR OMA; EDTIWEC; -.
DR OrthoDB; 1292821at2759; -.
DR TreeFam; TF101128; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000001811; Chromosome 3.
DR Bgee; ENSOCUG00000015774; Expressed in blood and 15 other tissues.
DR GO; GO:0033503; C:HULC complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell membrane; DNA damage; DNA repair; Membrane;
KW Nucleotide-binding; Nucleus; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..152
FT /note="Ubiquitin-conjugating enzyme E2 B"
FT /id="PRO_0000082449"
FT DOMAIN 4..150
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 88
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 152 AA; 17312 MW; CFDEEEE7E06840BE CRC64;
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS
EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS
PANSQAAQLY QENKREYEKR VSAIVEQSWN DS
