UBE2B_RAT
ID UBE2B_RAT Reviewed; 152 AA.
AC P63149; P23567; Q9D0J6;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 1.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 B;
DE EC=2.3.2.23;
DE AltName: Full=E2 ubiquitin-conjugating enzyme B;
DE AltName: Full=RAD6 homolog B;
DE Short=HR6B;
DE AltName: Full=Ubiquitin carrier protein B;
DE AltName: Full=Ubiquitin-conjugating enzyme E2(14k);
DE AltName: Full=Ubiquitin-protein ligase B;
GN Name=Ube2b; Synonyms=Rad6b;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=1313008; DOI=10.1016/s0021-9258(19)50455-0;
RA Wing S.S., Dumas F., Banville D.;
RT "A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin-
RT dependent proteolysis (E214k) is homologous to the yeast DNA repair gene
RT RAD6.";
RL J. Biol. Chem. 267:6495-6501(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND TISSUE SPECIFICITY.
RX PubMed=8048511; DOI=10.1152/ajpendo.1994.267.1.e39;
RA Wing S.S., Banville D.;
RT "14-kDa ubiquitin-conjugating enzyme: structure of the rat gene and
RT regulation upon fasting and by insulin.";
RL Am. J. Physiol. 267:E39-E48(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Brain;
RA Chen D., Graham S.H., Jing K., Simon R.P., Chen J.;
RT "Isolation of a ubiquitin-conjugating enzyme gene induced following
RT cerebral ischemia from the rat brain using modified subtractive
RT hybridization and differential screening.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INDUCTION, SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=15946855; DOI=10.1016/j.mcn.2005.04.008;
RA Kavakebi P., Hausott B., Tomasino A., Ingorokva S., Klimaschewski L.;
RT "The N-end rule ubiquitin-conjugating enzyme, HR6B, is up-regulated by
RT nerve growth factor and required for neurite outgrowth.";
RL Mol. Cell. Neurosci. 29:559-568(2005).
CC -!- FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its
CC covalent attachment to other proteins. In association with the E3
CC enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription
CC regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-
CC 120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic
CC transcriptional activation, elongation by RNA polymerase II, telomeric
CC silencing, and is also a prerequisite for H3K4me and H3K79me formation
CC (By similarity). In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and
CC 'Lys-63'-linked polyubiquitination. Required for postreplication repair
CC of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-
CC RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-
CC associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
CC {ECO:0000250|UniProtKB:P63146, ECO:0000269|PubMed:1313008,
CC ECO:0000269|PubMed:15946855}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC Evidence={ECO:0000250|UniProtKB:P63146, ECO:0000255|PROSITE-
CC ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC -!- SUBUNIT: Interacts with RAD18, UBR2 and WAC.
CC {ECO:0000250|UniProtKB:P63146}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15946855}.
CC Nucleus {ECO:0000269|PubMed:15946855}. Note=In peripheral neurons,
CC expressed both at the plasma membrane and in nuclei.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in testes, moderate levels
CC in muscle, heart, and brain, and low levels in liver and kidney. Upon
CC fasting, increased levels were seen in muscle, heart, liver, and
CC kidney. {ECO:0000269|PubMed:8048511}.
CC -!- INDUCTION: Up-regulated by NGF. {ECO:0000269|PubMed:15946855}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC {ECO:0000255|PROSITE-ProRule:PRU00388}.
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DR EMBL; M62388; AAA21087.1; -; mRNA.
DR EMBL; U04308; AAB60669.1; -; Genomic_DNA.
DR EMBL; U04303; AAB60669.1; JOINED; Genomic_DNA.
DR EMBL; U04304; AAB60669.1; JOINED; Genomic_DNA.
DR EMBL; U04305; AAB60669.1; JOINED; Genomic_DNA.
DR EMBL; U04306; AAB60669.1; JOINED; Genomic_DNA.
DR EMBL; U04307; AAB60669.1; JOINED; Genomic_DNA.
DR EMBL; AF144083; AAD37966.1; -; mRNA.
DR EMBL; BC070946; AAH70946.1; -; mRNA.
DR PIR; I51913; I51913.
DR RefSeq; NP_112400.1; NM_031138.2.
DR AlphaFoldDB; P63149; -.
DR BMRB; P63149; -.
DR SMR; P63149; -.
DR BioGRID; 249674; 1.
DR STRING; 10116.ENSRNOP00000016742; -.
DR iPTMnet; P63149; -.
DR PhosphoSitePlus; P63149; -.
DR jPOST; P63149; -.
DR PaxDb; P63149; -.
DR GeneID; 81816; -.
DR KEGG; rno:81816; -.
DR UCSC; RGD:708345; rat.
DR CTD; 7320; -.
DR RGD; 708345; Ube2b.
DR eggNOG; KOG0419; Eukaryota.
DR HOGENOM; CLU_030988_10_2_1; -.
DR InParanoid; P63149; -.
DR PhylomeDB; P63149; -.
DR TreeFam; TF101128; -.
DR Reactome; R-RNO-110314; Recognition of DNA damage by PCNA-containing replication complex.
DR Reactome; R-RNO-8866652; Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
DR Reactome; R-RNO-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR Reactome; R-RNO-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR PRO; PR:P63149; -.
DR Proteomes; UP000002494; Unplaced.
DR Genevisible; P63149; RN.
DR GO; GO:0000785; C:chromatin; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0033503; C:HULC complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005657; C:replication fork; ISO:RGD.
DR GO; GO:0001741; C:XY body; ISO:RGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; ISO:RGD.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; ISO:RGD.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISO:RGD.
DR GO; GO:0032869; P:cellular response to insulin stimulus; IEP:RGD.
DR GO; GO:0051026; P:chiasma assembly; ISO:RGD.
DR GO; GO:0006281; P:DNA repair; ISO:RGD.
DR GO; GO:0033522; P:histone H2A ubiquitination; ISO:RGD.
DR GO; GO:0070076; P:histone lysine demethylation; ISO:RGD.
DR GO; GO:0016574; P:histone ubiquitination; IBA:GO_Central.
DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR GO; GO:0045141; P:meiotic telomere clustering; ISO:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD.
DR GO; GO:0043951; P:negative regulation of cAMP-mediated signaling; ISO:RGD.
DR GO; GO:0033128; P:negative regulation of histone phosphorylation; ISO:RGD.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; ISO:RGD.
DR GO; GO:0010845; P:positive regulation of reciprocal meiotic recombination; ISO:RGD.
DR GO; GO:0006301; P:postreplication repair; ISO:RGD.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISO:RGD.
DR GO; GO:0051865; P:protein autoubiquitination; ISO:RGD.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
DR GO; GO:0006513; P:protein monoubiquitination; ISO:RGD.
DR GO; GO:0000209; P:protein polyubiquitination; ISO:RGD.
DR GO; GO:0050821; P:protein stabilization; ISO:RGD.
DR GO; GO:0016567; P:protein ubiquitination; ISO:RGD.
DR GO; GO:0031056; P:regulation of histone modification; ISO:RGD.
DR GO; GO:0001666; P:response to hypoxia; IEP:RGD.
DR GO; GO:0009411; P:response to UV; ISO:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; ISO:RGD.
DR GO; GO:0007288; P:sperm axoneme assembly; ISO:RGD.
DR GO; GO:0007283; P:spermatogenesis; ISO:RGD.
DR GO; GO:0070193; P:synaptonemal complex organization; ISO:RGD.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEP:RGD.
DR CDD; cd00195; UBCc; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR000608; UBQ-conjugat_E2.
DR InterPro; IPR023313; UBQ-conjugating_AS.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF00179; UQ_con; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS00183; UBC_1; 1.
DR PROSITE; PS50127; UBC_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell membrane; DNA damage; DNA repair; Membrane;
KW Nucleotide-binding; Nucleus; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..152
FT /note="Ubiquitin-conjugating enzyme E2 B"
FT /id="PRO_0000082450"
FT DOMAIN 4..150
FT /note="UBC core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT ACT_SITE 88
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT ECO:0000255|PROSITE-ProRule:PRU10133"
SQ SEQUENCE 152 AA; 17312 MW; CFDEEEE7E06840BE CRC64;
MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS
EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS
PANSQAAQLY QENKREYEKR VSAIVEQSWN DS
